Article ; Online: A β-glucuronidase (GUS) Based Bacterial Competition Assay to Assess Fine Differencesin Fitness during Plant Infection.
2022 Volume 12, Issue 13, Page(s) e3776
Abstract: Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences ... ...
Abstract | Competition assays are a simple phenotyping strategy that confront two bacterial strains to evaluate their relative fitness. Because they are more accurate than single-strain growth assays, competition assays can be used to highlight slight differences that would not otherwise be detectable. In the frame of host-pathogens interactions, they can be very useful to study the contribution of individual bacterial genes to bacterial fitness and lead to the identification of new adaptive traits. Here, we describe how to perform such competition assays by taking the example of the model phytopathogenic bacterium |
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Language | English |
Publishing date | 2022-07-05 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 2833269-6 |
ISSN | 2331-8325 ; 2331-8325 |
ISSN (online) | 2331-8325 |
ISSN | 2331-8325 |
DOI | 10.21769/BioProtoc.3776 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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