Artikel ; Online: A Selection of Macrocyclic Peptides That Bind STING From an mRNA-Display Library With Split Degenerate Codons.
Angewandte Chemie (International ed. in English)
2021 Band 60, Heft 42, Seite(n) 22640–22645
Abstract: Recent improvements in mRNA display have enabled the selection of peptides that incorporate non-natural amino acids, thus expanding the chemical diversity of macrocycles beyond what is accessible in nature. Such libraries have incorporated non-natural ... ...
Abstract | Recent improvements in mRNA display have enabled the selection of peptides that incorporate non-natural amino acids, thus expanding the chemical diversity of macrocycles beyond what is accessible in nature. Such libraries have incorporated non-natural amino acids at the expense of natural amino acids by reassigning their codons. Here we report an alternative approach to expanded amino-acid diversity that preserves all 19 natural amino acids (no methionine) and adds 6 non-natural amino acids, resulting in the highest sequence complexity reported to date. We have applied mRNA display to this 25-letter library to select functional macrocycles that bind human STING, a protein involved in immunoregulation. The resulting STING-binding peptides include a 9-mer macrocycle with a dissociation constant (K |
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Mesh-Begriff(e) | Amino Acid Sequence ; Amino Acids/chemistry ; Codon ; Cyclic AMP/chemistry ; Cyclic AMP/metabolism ; Cyclic GMP/chemistry ; Cyclic GMP/metabolism ; Dimerization ; Genetic Engineering ; Humans ; Kinetics ; Membrane Proteins/chemistry ; Membrane Proteins/metabolism ; Peptide Library ; Peptides, Cyclic/chemistry ; Peptides, Cyclic/metabolism ; RNA, Messenger/genetics ; RNA, Messenger/metabolism |
Chemische Substanzen | Amino Acids ; Codon ; Membrane Proteins ; Peptide Library ; Peptides, Cyclic ; RNA, Messenger ; STING1 protein, human ; Cyclic AMP (E0399OZS9N) ; Cyclic GMP (H2D2X058MU) |
Sprache | Englisch |
Erscheinungsdatum | 2021-09-06 |
Erscheinungsland | Germany |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2011836-3 |
ISSN | 1521-3773 ; 1433-7851 |
ISSN (online) | 1521-3773 |
ISSN | 1433-7851 |
DOI | 10.1002/anie.202103043 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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