Artikel ; Online: 4-Octyl itaconate inhibits aerobic glycolysis by targeting GAPDH to exert anti-inflammatory effects.
2019 Band 10, Heft 1, Seite(n) 5091
Abstract: Activated macrophages switch from oxidative phosphorylation to aerobic glycolysis, similar to the Warburg effect, presenting a potential therapeutic target in inflammatory disease. The endogenous metabolite itaconate has been reported to regulate ... ...
Abstract | Activated macrophages switch from oxidative phosphorylation to aerobic glycolysis, similar to the Warburg effect, presenting a potential therapeutic target in inflammatory disease. The endogenous metabolite itaconate has been reported to regulate macrophage function, but its precise mechanism is not clear. Here, we show that 4-octyl itaconate (4-OI, a cell-permeable itaconate derivative) directly alkylates cysteine residue 22 on the glycolytic enzyme GAPDH and decreases its enzyme activity. Glycolytic flux analysis by U |
---|---|
Mesh-Begriff(e) | Alkylation ; Animals ; Antimetabolites/pharmacology ; Cysteine/drug effects ; Cysteine/genetics ; Cysteine/metabolism ; Deoxyglucose/pharmacology ; Down-Regulation ; Endotoxemia/immunology ; Glucose/metabolism ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/antagonists & inhibitors ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/drug effects ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)/metabolism ; Glycolysis/drug effects ; Inflammation/immunology ; Interleukin-1beta/drug effects ; Interleukin-1beta/immunology ; Lipopolysaccharides/pharmacology ; Macrophage Activation/immunology ; Macrophages/drug effects ; Macrophages/immunology ; Macrophages/metabolism ; Mice ; Nitric Oxide Synthase Type II/drug effects ; Nitric Oxide Synthase Type II/immunology ; Oxidative Phosphorylation/drug effects ; RAW 264.7 Cells ; Sesquiterpenes/pharmacology ; Succinates/pharmacology |
Chemische Substanzen | Antimetabolites ; IL1B protein, mouse ; Interleukin-1beta ; Lipopolysaccharides ; Sesquiterpenes ; Succinates ; heptelidic acid (57710-57-3) ; Deoxyglucose (9G2MP84A8W) ; Nitric Oxide Synthase Type II (EC 1.14.13.39) ; Nos2 protein, mouse (EC 1.14.13.39) ; Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) (EC 1.2.1.12) ; Glucose (IY9XDZ35W2) ; Cysteine (K848JZ4886) ; itaconic acid (Q4516562YH) |
Sprache | Englisch |
Erscheinungsdatum | 2019-11-08 |
Erscheinungsland | England |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2553671-0 |
ISSN | 2041-1723 ; 2041-1723 |
ISSN (online) | 2041-1723 |
ISSN | 2041-1723 |
DOI | 10.1038/s41467-019-13078-5 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.