Artikel ; Online: RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis.
2023 Band 51, Heft 14, Seite(n) 7357–7375
Abstract: DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme ...
Abstract | DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme that degrades the RNA within DNA-RNA hybrids, impairs spermatogenesis and causes male infertility. Notably, Rnaseh1 knockout results in incomplete DNA repair and meiotic prophase I arrest. These defects arise from the altered RAD51 and DMC1 recruitment in zygotene spermatocytes. Furthermore, single-molecule experiments show that RNase H1 promotes recombinase recruitment to DNA by degrading RNA within DNA-RNA hybrids and allows nucleoprotein filaments formation. Overall, we uncover a function of RNase H1 in meiotic recombination, during which it processes DNA-RNA hybrids and facilitates recombinase recruitment. |
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Mesh-Begriff(e) | Humans ; Male ; Cell Cycle Proteins/genetics ; Cell Cycle Proteins/metabolism ; DNA/genetics ; DNA/metabolism ; Meiosis ; Rad51 Recombinase/genetics ; Rad51 Recombinase/metabolism ; Recombinases/genetics ; Spermatocytes/metabolism ; Ribonuclease H/metabolism |
Chemische Substanzen | Cell Cycle Proteins ; DNA (9007-49-2) ; Rad51 Recombinase (EC 2.7.7.-) ; Recombinases ; ribonuclease HI (EC 3.1.26.4) ; Ribonuclease H (EC 3.1.26.4) |
Sprache | Englisch |
Erscheinungsdatum | 2023-08-14 |
Erscheinungsland | England |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 186809-3 |
ISSN | 1362-4962 ; 1362-4954 ; 0301-5610 ; 0305-1048 |
ISSN (online) | 1362-4962 ; 1362-4954 |
ISSN | 0301-5610 ; 0305-1048 |
DOI | 10.1093/nar/gkad524 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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