LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Lv, Linfen"
  2. AU="Jason S. Stumhofer" AU="Jason S. Stumhofer"

Suchergebnis

Treffer 1 - 2 von insgesamt 2

Suchoptionen

  1. Artikel ; Online: Both LTA and LTB Subunits Are Equally Important to Heat-Labile Enterotoxin (LT)-Enhanced Bacterial Adherence.

    Duan, Qiangde / Pang, Shengmei / Feng, Lili / Li, Baoliang / Lv, Linfen / Liang, Yuxuan / Zhu, Guoqiang

    International journal of molecular sciences

    2023  Band 24, Heft 2

    Abstract: There is increasing evidence indicating that the production of heat-labile enterotoxin (LT) enhances bacterial adherence within in vitro and in vivo models. However, which subunit plays the main role, and the precise regulatory mechanisms remain unclear. ...

    Abstract There is increasing evidence indicating that the production of heat-labile enterotoxin (LT) enhances bacterial adherence within in vitro and in vivo models. However, which subunit plays the main role, and the precise regulatory mechanisms remain unclear. To further elucidate the contribution of the A subunit of LT (LTA) and the B subunit of LT (LTB) in LT-enhanced bacterial adherence, we generated several LT mutants where their ADP-ribosylation activity or GM1 binding ability was impaired and evaluated their abilities to enhance the two LT-deficient
    Mesh-Begriff(e) Enterotoxins/genetics ; Enterotoxins/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism ; Hot Temperature ; G(M1) Ganglioside ; Escherichia coli Proteins/genetics
    Chemische Substanzen Enterotoxins ; Bacterial Toxins ; G(M1) Ganglioside (37758-47-7) ; Escherichia coli Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-01-08
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24021245
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel ; Online: Heat-labile enterotoxin enhances F4-producing enterotoxigenic E. coli adhesion to porcine intestinal epithelial cells by upregulating bacterial adhesins and STb enterotoxin.

    Duan, Qiangde / Pang, Shengmei / Feng, Lili / Liu, Jiaqi / Lv, Linfen / Li, Baoliang / Liang, Yuxuan / Zhu, Guoqiang

    Veterinary research

    2022  Band 53, Heft 1, Seite(n) 88

    Abstract: As one of the crucial enterotoxins secreted by enterotoxigenic Escherichia coli (ETEC), heat-labile enterotoxin (LT) enhances bacterial adherence both in vivo and in vitro; however, the underlying mechanism remains unclear. To address this, we evaluated ... ...

    Abstract As one of the crucial enterotoxins secreted by enterotoxigenic Escherichia coli (ETEC), heat-labile enterotoxin (LT) enhances bacterial adherence both in vivo and in vitro; however, the underlying mechanism remains unclear. To address this, we evaluated the adherence of LT-producing and LT-deficient ETEC strains using the IPEC-J2 cell model. The expression levels of inflammatory cytokines and chemokines, and tight-junction proteins were evaluated in IPEC-J2 cells after infection with various ETEC strains. Further, the levels of adhesins and enterotoxins were also evaluated in F4ac-producing ETEC (F4 + ETEC) strains after treatment with cyclic AMP (cAMP). The adherence of the ΔeltAB mutant was decreased compared with the wild-type strain, whereas adherence of the 1836-2/pBR322-eltAB strain was markedly increased compared with the 1836-2 parental strain. Production of LT up-regulated the expression of TNF-α, IL-6, CXCL-8, and IL-10 genes. However, it did not appear to affect tight junction protein expression. Importantly, we found that cAMP leads to the upregulation of adhesin production and STb enterotoxin. Moreover, the F4 + ETEC strains treated with cAMP also had greater adhesion to IPEC-J2 cells, and the adherence of ΔfaeG, ΔfliC, and ΔestB mutants was decreased. These results indicate that LT enhances the adherence of F4 + ETEC due primarily to the upregulation of F4 fimbriae, flagellin, and STb enterotoxin expression and provide insights into the pathogenic mechanism of LT and ETEC.
    Mesh-Begriff(e) Animals ; Adhesins, Bacterial/genetics ; Adhesins, Bacterial/metabolism ; Diarrhea/microbiology ; Diarrhea/veterinary ; Enterotoxigenic Escherichia coli/physiology ; Enterotoxins/genetics ; Enterotoxins/metabolism ; Epithelial Cells/microbiology ; Escherichia coli Infections/microbiology ; Escherichia coli Infections/veterinary ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Swine ; Swine Diseases/microbiology ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism
    Chemische Substanzen Adhesins, Bacterial ; Enterotoxins ; Escherichia coli Proteins ; heat-labile enterotoxin, E coli (D9K3SN2LNY) ; Bacterial Toxins
    Sprache Englisch
    Erscheinungsdatum 2022-10-27
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1146298-x
    ISSN 1297-9716 ; 0928-4249
    ISSN (online) 1297-9716
    ISSN 0928-4249
    DOI 10.1186/s13567-022-01110-4
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.B 1313: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

Zum Seitenanfang