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  1. Artikel: Genetic loci associated with tissue-specific resistance to powdery mildew in octoploid strawberry (

    Lynn, Samantha C / Dunwell, Jim M / Whitehouse, Adam B / Cockerton, Helen M

    Frontiers in plant science

    2024  Band 15, Seite(n) 1376061

    Abstract: Powdery mildew is one of the most problematic diseases in strawberry production. To date, few commercial strawberry cultivars are deemed to have complete resistance and as such, an extensive spray programme must be implemented to control the pathogen. ... ...

    Abstract Powdery mildew is one of the most problematic diseases in strawberry production. To date, few commercial strawberry cultivars are deemed to have complete resistance and as such, an extensive spray programme must be implemented to control the pathogen. Here, a large-scale field experiment was used to determine the powdery mildew resistance status of leaf and fruit tissues across a diverse panel of strawberry genotypes. This phenotypic data was used to identify Quantitative Trait Nucleotides (QTN) associated with tissue-specific powdery mildew resistance. In total, six stable QTN were found to be associated with foliar resistance, with one QTN on chromosome 7D associated with a 61% increase in resistance. In contrast to the foliage results, there were no QTN associated with fruit disease resistance and there was a high level of resistance observed on strawberry fruit, with no genetic correlation observed between fruit and foliar symptoms, indicating a tissue-specific response. Beyond the identification of genetic loci, we also demonstrate that genomic selection can lead to rapid gains in foliar resistance across genotypes, with the potential to capture >50% of the genetic foliage resistance present in the population. To date, breeding of robust powdery mildew resistance in strawberry has been impeded by the quantitative nature of natural resistance and a lack of knowledge relating to the genetic control of the trait. These results address this shortfall, through providing the community with a wealth of information that could be utilized for genomic informed breeding, implementation of which could deliver a natural resistance strategy for combatting powdery mildew.
    Sprache Englisch
    Erscheinungsdatum 2024-04-29
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2024.1376061
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Genetic dissection of the tissue-specific roles of type III effectors and phytotoxins in the pathogenicity of Pseudomonas syringae pv. syringae to cherry.

    Vadillo-Dieguez, Andrea / Zeng, Ziyue / Mansfield, John W / Grinberg, Nastasiya F / Lynn, Samantha C / Gregg, Adam / Connell, John / Harrison, Richard J / Jackson, Robert W / Hulin, Michelle T

    Molecular plant pathology

    2024  Band 25, Heft 4, Seite(n) e13451

    Abstract: When compared with other phylogroups (PGs) of the Pseudomonas syringae species complex, P. syringae pv. syringae (Pss) strains within PG2 have a reduced repertoire of type III effectors (T3Es) but produce several phytotoxins. Effectors within the cherry ... ...

    Abstract When compared with other phylogroups (PGs) of the Pseudomonas syringae species complex, P. syringae pv. syringae (Pss) strains within PG2 have a reduced repertoire of type III effectors (T3Es) but produce several phytotoxins. Effectors within the cherry pathogen Pss 9644 were grouped based on their frequency in strains from Prunus as the conserved effector locus (CEL) common to most P. syringae pathogens; a core of effectors common to PG2; a set of PRUNUS effectors common to cherry pathogens; and a FLEXIBLE set of T3Es. Pss 9644 also contains gene clusters for biosynthesis of toxins syringomycin, syringopeptin and syringolin A. After confirmation of virulence gene expression, mutants with a sequential series of T3E and toxin deletions were pathogenicity tested on wood, leaves and fruits of sweet cherry (Prunus avium) and leaves of ornamental cherry (Prunus incisa). The toxins had a key role in disease development in fruits but were less important in leaves and wood. An effectorless mutant retained some pathogenicity to fruit but not wood or leaves. Striking redundancy was observed amongst effector groups. The CEL effectors have important roles during the early stages of leaf infection and possibly acted synergistically with toxins in all tissues. Deletion of separate groups of T3Es had more effect in P. incisa than in P. avium. Mixed inocula were used to complement the toxin mutations in trans and indicated that strain mixtures may be important in the field. Our results highlight the niche-specific role of toxins in P. avium tissues and the complexity of effector redundancy in the pathogen Pss 9644.
    Mesh-Begriff(e) Virulence/genetics ; Pseudomonas syringae ; Prunus avium/metabolism ; Fruit/metabolism ; Mutation/genetics ; Prunus/metabolism ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
    Chemische Substanzen Bacterial Proteins
    Sprache Englisch
    Erscheinungsdatum 2024-04-08
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2020755-4
    ISSN 1364-3703 ; 1364-3703
    ISSN (online) 1364-3703
    ISSN 1364-3703
    DOI 10.1111/mpp.13451
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Chromosome-scale genome sequence assemblies of the 'Autumn Bliss' and 'Malling Jewel' cultivars of the highly heterozygous red raspberry (Rubus idaeus L.) derived from long-read Oxford Nanopore sequence data.

    Price, R Jordan / Davik, Jahn / Fernandéz Fernandéz, Felicidad / Bates, Helen J / Lynn, Samantha / Nellist, Charlotte F / Buti, Matteo / Røen, Dag / Šurbanovski, Nada / Alsheikh, Muath / Harrison, Richard J / Sargent, Daniel James

    PloS one

    2023  Band 18, Heft 5, Seite(n) e0285756

    Abstract: Red raspberry (Rubus idaeus L.) is an economically valuable soft-fruit species with a relatively small (~300 Mb) but highly heterozygous diploid (2n = 2x = 14) genome. Chromosome-scale genome sequences are a vital tool in unravelling the genetic ... ...

    Abstract Red raspberry (Rubus idaeus L.) is an economically valuable soft-fruit species with a relatively small (~300 Mb) but highly heterozygous diploid (2n = 2x = 14) genome. Chromosome-scale genome sequences are a vital tool in unravelling the genetic complexity controlling traits of interest in crop plants such as red raspberry, as well as for functional genomics, evolutionary studies, and pan-genomics diversity studies. In this study, we developed genome sequences of a primocane fruiting variety ('Autumn Bliss') and a floricane variety ('Malling Jewel'). The use of long-read Oxford Nanopore Technologies sequencing data yielded long read lengths that permitted well resolved genome sequences for the two cultivars to be assembled. The de novo assemblies of 'Malling Jewel' and 'Autumn Bliss' contained 79 and 136 contigs respectively, and 263.0 Mb of the 'Autumn Bliss' and 265.5 Mb of the 'Malling Jewel' assembly could be anchored unambiguously to a previously published red raspberry genome sequence of the cultivar 'Anitra'. Single copy ortholog analysis (BUSCO) revealed high levels of completeness in both genomes sequenced, with 97.4% of sequences identified in 'Autumn Bliss' and 97.7% in 'Malling Jewel'. The density of repetitive sequence contained in the 'Autumn Bliss' and 'Malling Jewel' assemblies was significantly higher than in the previously published assembly and centromeric and telomeric regions were identified in both assemblies. A total of 42,823 protein coding regions were identified in the 'Autumn Bliss' assembly, whilst 43,027 were identified in the 'Malling Jewel' assembly. These chromosome-scale genome sequences represent an excellent genomics resource for red raspberry, particularly around the highly repetitive centromeric and telomeric regions of the genome that are less complete in the previously published 'Anitra' genome sequence.
    Mesh-Begriff(e) Rubus/genetics ; Nanopores ; Genome ; Genomics ; Sequence Analysis, DNA ; Centromere
    Sprache Englisch
    Erscheinungsdatum 2023-05-16
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0285756
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Identifying resistance in wild and ornamental cherry towards bacterial canker caused by Pseudomonas syringae

    Hulin, Michelle T. / Vadillo Dieguez, Andrea / Cossu, Francesca / Lynn, Samantha / Russell, Karen / Neale, Helen C. / Jackson, Robert W. / Arnold, Dawn L. / Mansfield, John W. / Harrison, Richard J.

    Plant pathology. 2022 May, v. 71, no. 4

    2022  

    Abstract: Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance ... ...

    Abstract Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance in sweet cherry is discernible using shoot or whole tree inoculations; however, these quantitative differences in resistance are not evident in detached leaf assays. To identify novel sources of resistance to canker, we used a rapid leaf pathogenicity test to screen a range of wild cherry, ornamental Prunus species and sweet cherry × ornamental cherry hybrids with the canker pathogens, Pseudomonas syringae pvs syringae, morsprunorum races 1 and 2, and avii. Several Prunus accessions exhibited limited symptom development following inoculation with each of the pathogens, and this resistance extended to 16 P. syringae strains pathogenic on sweet cherry and plum. Resistance was associated with reduced bacterial multiplication after inoculation, a phenotype similar to that of commercial sweet cherry towards nonhost strains of P. syringae. Progeny resulting from a cross of a resistant ornamental species Prunus incisa with susceptible sweet cherry (P. avium) exhibited resistance indicating it is an inherited trait. Identification of accessions with resistance to the major bacterial canker pathogens is the first step towards characterizing the underlying genetic mechanisms of resistance and introducing these traits into commercial germplasm.
    Schlagwörter Prunus avium ; Prunus incisa ; Pseudomonas syringae ; bacterial canker ; cherries ; disease control ; germplasm ; leaves ; pathogenicity ; phenotype ; plant pathology ; plums ; progeny ; trees ; varietal resistance
    Sprache Englisch
    Erscheinungsverlauf 2022-05
    Umfang p. 949-965.
    Erscheinungsort John Wiley & Sons, Ltd
    Dokumenttyp Artikel
    Anmerkung JOURNAL ARTICLE
    ZDB-ID 415941-x
    ISSN 1365-3059 ; 0032-0862
    ISSN (online) 1365-3059
    ISSN 0032-0862
    DOI 10.1111/ppa.13513
    Datenquelle NAL Katalog (AGRICOLA)

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  5. Artikel: Identifying resistance in wild and ornamental cherry towards bacterial canker caused by

    Hulin, Michelle T / Vadillo Dieguez, Andrea / Cossu, Francesca / Lynn, Samantha / Russell, Karen / Neale, Helen C / Jackson, Robert W / Arnold, Dawn L / Mansfield, John W / Harrison, Richard J

    Plant pathology

    2021  Band 71, Heft 4, Seite(n) 949–965

    Abstract: Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry ( ...

    Abstract Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (
    Sprache Englisch
    Erscheinungsdatum 2021-12-21
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 415941-x
    ISSN 1365-3059 ; 0032-0862
    ISSN (online) 1365-3059
    ISSN 0032-0862
    DOI 10.1111/ppa.13513
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel ; Online: Identifying resistance in wild and ornamental cherry towards bacterial canker caused by Pseudomonas syringae

    Hulin, Michelle T. / Vadillo Dieguez, Andrea / Cossu, Francesca / Lynn, Samantha / Russell, Karen / Neale, Helen C. / Jackson, Robert W. / Arnold, Dawn L. / Mansfield, John W. / Harrison, Richard J.

    Plant Pathology

    2022  Band 71, Heft 4

    Abstract: Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance ... ...

    Abstract Bacterial canker is a major disease of stone fruits and is a critical limiting factor to sweet cherry (Prunus avium) production worldwide. One important strategy for disease control is the development of resistant varieties. Partial varietal resistance in sweet cherry is discernible using shoot or whole tree inoculations; however, these quantitative differences in resistance are not evident in detached leaf assays. To identify novel sources of resistance to canker, we used a rapid leaf pathogenicity test to screen a range of wild cherry, ornamental Prunus species and sweet cherry × ornamental cherry hybrids with the canker pathogens, Pseudomonas syringae pvs syringae, morsprunorum races 1 and 2, and avii. Several Prunus accessions exhibited limited symptom development following inoculation with each of the pathogens, and this resistance extended to 16 P. syringae strains pathogenic on sweet cherry and plum. Resistance was associated with reduced bacterial multiplication after inoculation, a phenotype similar to that of commercial sweet cherry towards nonhost strains of P. syringae. Progeny resulting from a cross of a resistant ornamental species Prunus incisa with susceptible sweet cherry (P. avium) exhibited resistance indicating it is an inherited trait. Identification of accessions with resistance to the major bacterial canker pathogens is the first step towards characterizing the underlying genetic mechanisms of resistance and introducing these traits into commercial germplasm.
    Schlagwörter Pseudomonas syringae ; disease resistance ; tree disease
    Thema/Rubrik (Code) 571
    Sprache Englisch
    Erscheinungsland nl
    Dokumenttyp Artikel ; Online
    ZDB-ID 415941-x
    ISSN 1365-3059 ; 0032-0862
    ISSN (online) 1365-3059
    ISSN 0032-0862
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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