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  1. Buch ; Dissertation / Habilitation: Ultrastructural characterization of mammalian k-fibers by large-scale electron tomography

    Kiewisz, Robert / Müller-Reichert, Thomas / Needleman, Daniel J.

    2021  

    Körperschaft Medizinische Klinik und Poliklinik III
    Verfasserangabe von Robert Kiewisz aus Olsztyn, Polen ; Medizinische Klinik und Poliklinik III [Dresden]
    Sprache Englisch
    Umfang 153 Blätter, Illustrationen, Diagramme
    Erscheinungsort Dresden
    Erscheinungsland Deutschland
    Dokumenttyp Buch ; Dissertation / Habilitation
    Dissertation / Habilitation Dissertation, Technische Universität Dresden, Medizinische Fakultät, 2021
    HBZ-ID HT021771588
    Datenquelle Katalog ZB MED Medizin, Gesundheit

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  2. Buch ; Dissertation / Habilitation: Support of kinetochore microtubule attachments by CENP-V is required for meiosis progression in mouse oocytes

    Nabi, Dalileh / Jessberger, Rolf / Müller-Reichert, Thomas

    2021  

    Körperschaft Institut für Physiologische Chemie
    Verfasserangabe von Dalileh Nabi, M.Sc. aus Teheran, Iran ; Institut für Physiologische Chemie [Dresden]
    Sprache Englisch
    Umfang vii, 81 Blätter, Illustrationen, Diagramme
    Erscheinungsort Dresden
    Erscheinungsland Deutschland
    Dokumenttyp Buch ; Dissertation / Habilitation
    Dissertation / Habilitation Dissertation, Technische Universität Dresden, Medizinische Fakultät, 2021
    HBZ-ID HT021013541
    Datenquelle Katalog ZB MED Medizin, Gesundheit

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  3. Buch ; Online ; E-Book: Three-dimensional electron microscopy

    Müller-Reichert, Thomas / Pigino, Gaia

    (Methods in cell biology ; 152)

    2019  

    Verfasserangabe edited by Thomas Muller-Reichert, Gaia Pigino
    Serientitel Methods in cell biology ; 152
    Überordnung
    Schlagwörter Electron microscopy ; Three-dimensional imaging in biology
    Thema/Rubrik (Code) 570.2825
    Sprache Englisch
    Umfang 1 Online-Ressource (xiii, 289 Seiten), Illustrationen, Diagramme
    Ausgabenhinweis First edition
    Verlag Elsevier AP Academic Press
    Erscheinungsort Cambridge, MA
    Erscheinungsland Vereinigte Staaten
    Dokumenttyp Buch ; Online ; E-Book
    Bemerkung Zugriff für angemeldete ZB MED-Nutzerinnen und -Nutzer
    HBZ-ID HT020205760
    ISBN 978-0-12-817019-9 ; 9780128170182 ; 0-12-817019-0 ; 0128170182
    Datenquelle ZB MED Katalog Medizin, Gesundheit, Ernährung, Umwelt, Agrar

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  4. Buch ; Dissertation / Habilitation: Development and application of image processing algorithms for the analysis of renal cell populations and function in mouse models

    Kessel, Friederike / Hugo, Christian / Müller-Reichert, Thomas

    2021  

    Körperschaft Medizinische Klinik und Poliklinik III
    Verfasserangabe von M.Sc. Friederike Kessel aus Wangen im Allgäu ; Medizinische Klinik und Poliklinik III [Dresden]
    Sprache Englisch
    Umfang 95 Seiten, Illustrationen, Diagramme
    Erscheinungsort Dresden
    Erscheinungsland Deutschland
    Dokumenttyp Buch ; Dissertation / Habilitation
    Dissertation / Habilitation Dissertation, Technische Universität Dresden, Medizinische Fakultät, 2022
    HBZ-ID HT021777260
    Datenquelle Katalog ZB MED Medizin, Gesundheit

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  5. Buch: Correlative light and electron microscopy

    Müller-Reichert, Thomas

    (Methods in cell biology ; 111)

    2012  

    Verfasserangabe ed. by Thomas Müller-Reichert
    Serientitel Methods in cell biology ; 111
    Überordnung
    Schlagwörter Microscopy ; Electron microscopy ; Molecular biology
    Thema/Rubrik (Code) 570.2823
    Sprache Englisch
    Umfang XIX, 404 S., [20] Bl. : Ill., graph. Darst., 24 cm
    Ausgabenhinweis 1. ed.
    Verlag Elsevier Acad. Press
    Erscheinungsort Amsterdam u.a.
    Erscheinungsland Niederlande
    Dokumenttyp Buch
    HBZ-ID HT017419107
    ISBN 978-0-12-416026-2 ; 0-12-416026-3
    Datenquelle Katalog ZB MED Medizin, Gesundheit

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  6. Buch: Electron microscopy of model systems

    Müller-Reichert, Thomas

    (Methods in cell biology ; 96)

    2010  

    Verfasserangabe ed. by Thomas Müller-Reichert
    Serientitel Methods in cell biology ; 96
    Überordnung
    Sprache Englisch
    Umfang XX, 724 S., [18] Bl. : Ill., graph. Darst., 26 cm
    Ausgabenhinweis 1. ed.
    Verlag Elsevier, Acad. Press
    Erscheinungsort Amsterdam u.a.
    Erscheinungsland Niederlande
    Dokumenttyp Buch
    HBZ-ID HT016545432
    ISBN 978-0-12-381007-6 ; 0-12-381007-8
    Datenquelle Katalog ZB MED Medizin, Gesundheit

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  7. Artikel: How to apply the broad toolbox of correlative light and electron microscopy to address a specific biological question.

    Tranfield, Erin M / Fabig, Gunar / Kurth, Thomas / Müller-Reichert, Thomas

    Methods in cell biology

    2024  Band 187, Seite(n) 1–41

    Abstract: Correlative light and electron microscopy (CLEM) is an approach that combines the strength of multiple imaging techniques to obtain complementary information about a given specimen. The "toolbox" for CLEM is broad, making it sometimes difficult to choose ...

    Abstract Correlative light and electron microscopy (CLEM) is an approach that combines the strength of multiple imaging techniques to obtain complementary information about a given specimen. The "toolbox" for CLEM is broad, making it sometimes difficult to choose an appropriate approach for a given biological question. In this chapter, we provide experimental details for three CLEM approaches that can help the interested reader in designing a personalized CLEM strategy for obtaining ultrastructural data by using transmission electron microscopy (TEM). First, we describe chemical fixation of cells grown on a solid support (broadest approach). Second, we apply high-pressure freezing/freeze substitution to describe cellular ultrastructure (cryo-immobilization approach). Third, we give a protocol for a ultrastructural labeling by immuno-electron microscopy (immuno-EM approach). In addition, we also describe how to overlay fluorescence and electron microscopy images, an approach that is applicable to each of the reported different CLEM strategies. Here we provide step-by step descriptions prior to discussing possible technical problems and variations of these three general schemes to suit different models or different biological questions. This chapter is written for electron microscopists that are new to CLEM and unsure how to begin. Therefore, our protocols are meant to provide basic information with further references that should help the reader get started with applying a tailored strategy for a specific CLEM experiment.
    Mesh-Begriff(e) Humans ; Microscopy, Electron, Transmission/methods ; Animals ; Cryoelectron Microscopy/methods ; Microscopy, Electron/methods ; Microscopy, Immunoelectron/methods ; Microscopy, Fluorescence/methods ; Freeze Substitution/methods
    Sprache Englisch
    Erscheinungsdatum 2024-03-26
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 0091-679X
    ISSN 0091-679X
    DOI 10.1016/bs.mcb.2024.02.030
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: The X chromosome still has a lot to reveal - revisiting Hermann Henking's work on firebugs.

    Paliulis, Leocadia / Fabig, Gunar / Müller-Reichert, Thomas

    Journal of cell science

    2023  Band 136, Heft 4

    Abstract: In 1891, the existence of an X chromosome was noted for the first time. Hermann Henking was studying spermatocyte divisions of the firebug Pyrrhocoris apterus and observed that one chromosome behaved differently than all of the rest of the chromosomes. ... ...

    Abstract In 1891, the existence of an X chromosome was noted for the first time. Hermann Henking was studying spermatocyte divisions of the firebug Pyrrhocoris apterus and observed that one chromosome behaved differently than all of the rest of the chromosomes. Henking called this chromosome 'Element x'. Henking's discovery of the X element (later called X chromosome) initiated more than a century of fascinating genetics and cell biology, forming the foundation of several avenues of research in biology. His work led to exploration of a number of questions in a wide range of model systems and very soon to the abandonment of the firebug as a model for studies on the behavior of chromosomes in meiosis. Here, we argue that studies on both bivalent and univalent chromosome behavior in general, and work on how to solve chromosome lagging to prevent aneuploidy in particular, should lead us back to using the firebug as a model for error correction during cell division.
    Mesh-Begriff(e) Humans ; Meiosis ; Chromosomes/genetics ; Aneuploidy
    Sprache Englisch
    Erscheinungsdatum 2023-02-15
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2993-2
    ISSN 1477-9137 ; 0021-9533
    ISSN (online) 1477-9137
    ISSN 0021-9533
    DOI 10.1242/jcs.260998
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Serial-section Electron Tomography and Quantitative Analysis of Microtubule Organization in 3D-reconstructed Mitotic Spindles.

    Kiewisz, Robert / Baum, Daniel / Müller-Reichert, Thomas / Fabig, Gunar

    Bio-protocol

    2023  Band 13, Heft 20, Seite(n) e4849

    Abstract: For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic ... ...

    Abstract For the analysis of cellular architecture during mitosis, nanometer resolution is needed to visualize the organization of microtubules in spindles. Here, we present a detailed protocol that can be used to produce 3D reconstructions of whole mitotic spindles in cells grown in culture. For this, we attach mammalian cells enriched in mitotic stages to sapphire discs. Our protocol further involves cryo-immobilization by high-pressure freezing, freeze-substitution, and resin embedding. We then use fluorescence light microscopy to stage select mitotic cells in the resin-embedded samples. This is followed by large-scale electron tomography to reconstruct the selected and staged mitotic spindles in 3D. The generated and stitched electron tomograms are then used to semi-automatically segment the microtubules for subsequent quantitative analysis of spindle organization. Thus, by providing a detailed correlative light and electron microscopy (CLEM) approach, we give cell biologists a toolset to streamline the 3D visualization and analysis of spindle microtubules (http://kiewisz.shinyapps.io/asga). In addition, we refer to a recently launched platform that allows for an interactive display of the 3D-reconstructed mitotic spindles (https://cfci.shinyapps.io/ASGA_3DViewer/). Key features • High-throughput screening of mitotic cells by correlative light and electron microscopy (CLEM). • Serial-section electron tomography of selected cells. • Visualization of mitotic spindles in 3D and quantitative analysis of microtubule organization.
    Sprache Englisch
    Erscheinungsdatum 2023-10-20
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2833269-6
    ISSN 2331-8325 ; 2331-8325
    ISSN (online) 2331-8325
    ISSN 2331-8325
    DOI 10.21769/BioProtoc.4849
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Automated Segmentation of 3D Cytoskeletal Filaments from Electron Micrographs with TARDIS.

    Kiewisz, Robert / Fabig, Gunar / Müller-Reichert, Thomas / Bepler, Tristan

    Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada

    2023  Band 29, Heft 29 Suppl 1, Seite(n) 970–972

    Sprache Englisch
    Erscheinungsdatum 2023-08-23
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1385710-1
    ISSN 1435-8115 ; 1431-9276
    ISSN (online) 1435-8115
    ISSN 1431-9276
    DOI 10.1093/micmic/ozad067.485
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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