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  1. Article: Differences in Bacterial Communities of Retail Raw Pork in Different Market Types in Hangzhou, China.

    Wang, Wen / Yi, Zhengkai / Cai, Wei / Ma, Jiele / Yang, Hua / Zhou, Min / Xiao, Xingning

    Foods (Basel, Switzerland)

    2023  Volume 12, Issue 18

    Abstract: Pork is widely consumed globally, and pigs' microbiota can potentially harbor foodborne pathogens. Contaminated pork in retail markets poses significant implications for food quality and safety. However, limited studies have compared pork microbiomes in ... ...

    Abstract Pork is widely consumed globally, and pigs' microbiota can potentially harbor foodborne pathogens. Contaminated pork in retail markets poses significant implications for food quality and safety. However, limited studies have compared pork microbiomes in various marketing environments. In this study, we utilized traditional microbial culture methods and high-throughput 16S rRNA sequencing to assess pathogen contamination and bacterial diversity in raw pork samples purchased from farmers' markets and two types of supermarkets (upscale and ordinary) in Hangzhou, China. Traditional microbial plate cultures identified
    Language English
    Publishing date 2023-09-07
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods12183357
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Quantitative characterization and dynamics of bacterial communities in ready-to-eat chicken using high-throughput sequencing combined with internal standard-based absolute quantification.

    Yi, Zhengkai / Qiu, Mengjia / Xiao, Xingning / Ma, Jiele / Yang, Hua / Wang, Wen

    Food microbiology

    2023  Volume 118, Page(s) 104419

    Abstract: Ready-to-eat (RTE) chicken products are prone to bacterial contamination, posing foodborne illness risks. High-throughput sequencing (HTS) has been widely used to study the distribution of pathogenic and spoilage bacteria in RTE chicken products but ... ...

    Abstract Ready-to-eat (RTE) chicken products are prone to bacterial contamination, posing foodborne illness risks. High-throughput sequencing (HTS) has been widely used to study the distribution of pathogenic and spoilage bacteria in RTE chicken products but lacks quantitative data on taxa abundances. In this study, we employed a method combining HTS with absolute quantification, using Edwardsiella tarda as an internal standard strain, to achieve the relative and absolute abundances of microbiota in RTE chicken products stored at 4 and 25 °C. The results showed that the addition of appropriate concentration of internal standard strains exhibited no significant impact on the structure composition, relative abundance, and absolute abundance of bacterial communities in chicken meat, achieving comprehensive absolute quantification in RTE chicken products. Furthermore, the absolute abundance of bacterial genera at the end of storage followed a log-normal distribution, with most genera having an absolute abundance between 10
    MeSH term(s) Animals ; Chickens ; Food Microbiology ; Colony Count, Microbial ; Listeria monocytogenes ; High-Throughput Nucleotide Sequencing
    Language English
    Publishing date 2023-10-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 50892-5
    ISSN 1095-9998 ; 0740-0020
    ISSN (online) 1095-9998
    ISSN 0740-0020
    DOI 10.1016/j.fm.2023.104419
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Dynamic Changes of Bacterial Communities and Microbial Association Networks in Ready-to-Eat Chicken Meat during Storage.

    Qiu, Mengjia / Xiao, Xingning / Xiao, Yingping / Ma, Jiele / Yang, Hua / Jiang, Han / Dong, Qingli / Wang, Wen

    Foods (Basel, Switzerland)

    2022  Volume 11, Issue 22

    Abstract: Ready-to-eat (RTE) chicken is a popular food in China, but its lack of food safety due to bacterial contamination remains a concern, and the dynamic changes of microbial association networks during storage are not fully understood. This study ... ...

    Abstract Ready-to-eat (RTE) chicken is a popular food in China, but its lack of food safety due to bacterial contamination remains a concern, and the dynamic changes of microbial association networks during storage are not fully understood. This study investigated the impact of storage time and temperature on bacterial compositions and microbial association networks in RTE chicken using 16S rDNA high-throughput sequencing. The results show that the predominant phyla present in all samples were
    Language English
    Publishing date 2022-11-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704223-6
    ISSN 2304-8158
    ISSN 2304-8158
    DOI 10.3390/foods11223733
    Database MEDical Literature Analysis and Retrieval System OnLINE

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