Artikel ; Online: Recombinant Anti-Müllerian Hormone (rAMH) for Stalling In Vitro Granulosa Cell Replication.
Reproductive sciences (Thousand Oaks, Calif.)
2020 Band 27, Heft 10, Seite(n) 1873–1878
Abstract: To investigate whether recombinant AMH (rAMH) is able to decrease cellular proliferation/apoptosis in luteinized granulosa cells (GCs) through hormonal regulation, a primary culture of GCs was established from GCs obtained at time of oocyte retrieval ... ...
Abstract | To investigate whether recombinant AMH (rAMH) is able to decrease cellular proliferation/apoptosis in luteinized granulosa cells (GCs) through hormonal regulation, a primary culture of GCs was established from GCs obtained at time of oocyte retrieval from follicular fluid of 3 patients. Cells were seeded in well cell culture plates at a density of 100,000 cells/well in medium and treated with rAMH 20 ng/ml (rAMH group), or phosphate-buffered saline (PBS-control group), for 24 h. Total RNA was extracted from all cells, followed by cDNA synthesis and real-time RT-PCR to quantify the expression levels of AMH, AMH-R2, FSH-R, inhibin B, cell proliferation (Ki67), and apoptosis (Caspase 3). We used independent sample t test (SPSS v25) and a p < 0.05 significance. Cellular expressions of AMH, AMH-R2, FSH-R, and inhibin B were reduced greater than 50% in the rAMH group, compared with that of the the control group (p ≤ 0.005 for all). Ki67 and Caspase3 were also reduced greater than 30% in the rAMH group (p ≤ 0.001 for both). Our findings show a direct inhibitory effect of AMH on luteinized GCs' expression of the major regulatory hormones, in addition to a significant decrease in markers of cell proliferation and apoptosis. These results confirm the inhibitory effects of AMH on follicular development. |
---|---|
Mesh-Begriff(e) | Anti-Mullerian Hormone/metabolism ; Anti-Mullerian Hormone/pharmacology ; Apoptosis/drug effects ; Caspase 3/metabolism ; Cell Proliferation/drug effects ; Female ; Follicular Fluid ; Granulosa Cells/cytology ; Granulosa Cells/drug effects ; Humans ; Inhibins/metabolism ; Oocyte Retrieval ; Receptors, FSH/metabolism ; Receptors, Peptide/metabolism ; Receptors, Transforming Growth Factor beta/metabolism ; Recombinant Proteins/pharmacology |
Chemische Substanzen | Receptors, FSH ; Receptors, Peptide ; Receptors, Transforming Growth Factor beta ; Recombinant Proteins ; anti-Mullerian hormone receptor ; inhibin B ; Inhibins (57285-09-3) ; Anti-Mullerian Hormone (80497-65-0) ; Caspase 3 (EC 3.4.22.-) |
Sprache | Englisch |
Erscheinungsdatum | 2020-07-02 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article |
ZDB-ID | 2276411-2 |
ISSN | 1933-7205 ; 1933-7191 |
ISSN (online) | 1933-7205 |
ISSN | 1933-7191 |
DOI | 10.1007/s43032-020-00206-6 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Volltext online
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
Zs.A 4113: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.