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  1. Artikel ; Online: Research on the Mechanism of Parent–Child Attachment to College Student Adversarial Growth

    Mi Tian / Ting Nie / Hengrui Liang

    International Journal of Environmental Research and Public Health, Vol 19, Iss 3847, p

    2022  Band 3847

    Abstract: This study explores the impact of parent–child attachment mechanisms on adversarial growth among Chinese students. After Chinese college students start independent life away from their parents, they face adversity on their own. However, their original ... ...

    Abstract This study explores the impact of parent–child attachment mechanisms on adversarial growth among Chinese students. After Chinese college students start independent life away from their parents, they face adversity on their own. However, their original family always influences students’ methods for dealing with adversity and how they grow and mature. A survey of 364 college students found that parental trust and communication have positive impacts on adversarial growth through the improvement of self-identity, while parental alienation reduces self-identity and contributes negative effects on the adversarial growth of college students. Internal control personality has a negative moderating effect between parental trust, parental communication, and adversarial growth and a positive moderating effect between parental alienation and adversarial growth. Low internal control personality therefore has a positive influence on parental trust and communication on adversarial growth and decreases the negative influence of parental alienation. A substitution effect between internal control personality and parental attachment was also found. Different child personality requires different type of parent–child attachment relationship to maximize their ability to handle future adversity.
    Schlagwörter parent–child attachment ; self-identity ; adversarial growth ; internal control personality ; college student ; Medicine ; R
    Thema/Rubrik (Code) 150
    Sprache Englisch
    Erscheinungsdatum 2022-03-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
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  2. Artikel ; Online: Construction of rBCG carrying the IL-2-BZLF1 fusion gene and its immunological function.

    Yu, Meimei / Mi, Tian / Lu, Jiaqi / Cui, Lixian / Xue, Qingjie / Xiong, Huabao / Li, Yinlong

    Applied microbiology and biotechnology

    2024  Band 108, Heft 1, Seite(n) 19

    Abstract: In this research, a recombinant Bacillus Calmette Guerin (rBCG) vector vaccine carrying a human IL-2 and EBV BZLF1 fusion gene (IL-2-BZLF1-rBCG) was constructed. The IL-2-BZLF1-rBCG construct was successfully generated and stably expressed the IL-2 and ... ...

    Abstract In this research, a recombinant Bacillus Calmette Guerin (rBCG) vector vaccine carrying a human IL-2 and EBV BZLF1 fusion gene (IL-2-BZLF1-rBCG) was constructed. The IL-2-BZLF1-rBCG construct was successfully generated and stably expressed the IL-2 and BZLF1 proteins. IL-2-BZLF1-rBCG activated the immune system and promoted the secretion of IFN-γ and TNF-α by CD4
    Mesh-Begriff(e) Humans ; Interleukin-2/genetics ; CD8-Positive T-Lymphocytes ; Mycobacterium bovis/genetics ; BCG Vaccine ; Neoplasms ; Trans-Activators/genetics
    Chemische Substanzen Interleukin-2 ; BCG Vaccine ; BZLF1 protein, Herpesvirus 4, Human ; Trans-Activators
    Sprache Englisch
    Erscheinungsdatum 2024-01-03
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-023-12851-3
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Icaritin activates p53 and inhibits aerobic glycolysis in liver cancer cells.

    Zhou, Xiangyang / Wu, Di / Mi, Tian / Li, Ruohan / Guo, Tao / Li, Wenjuan

    Chemico-biological interactions

    2024  Band 392, Seite(n) 110926

    Abstract: Metabolic reprogramming enables cancer cells to generate energy mainly through aerobic glycolysis, which is achieved by increasing the expression levels of glycolysis-related enzymes. Therefore, the development of drugs targeting aerobic glycolysis could ...

    Abstract Metabolic reprogramming enables cancer cells to generate energy mainly through aerobic glycolysis, which is achieved by increasing the expression levels of glycolysis-related enzymes. Therefore, the development of drugs targeting aerobic glycolysis could be an effective strategy for cancer treatment. Icaritin (ICT) is an active ingredient from the Chinese herbal plant Epimedium with several biological activities, but its anti-cancer mechanism remains inconclusive. Using normal hepatocytes and hepatoma cells, our results showed that ICT suppressed cell proliferation and clonal formation and decreased glucose consumption and lactate production in liver cancer cells. In consistent, the mRNA and protein levels of several aerobic glycolysis-related genes were decreased upon ICT treatment. Furthermore, our results demonstrated that the expression levels of the aerobic glycolysis-related proteins were correlated with the p53 status in hepatoma cells. Using PFT-α or siRNA-p53, our results confirmed that ICT regulated aerobic glycolysis in a p53-dependent manner. In addition, ICT was found to stabilize p53 at the post-translational level which might be mediated by inhibiting MDM2 expression and affecting its interaction with p53. Finally, our results demonstrated that ICT increased the levels of ROS that activated p53 via the p38 MAPK pathway. In conclusion, ICT increased intracellular ROS levels in liver cancer cells, which promoted the stabilization and activation of p53, inhibiting the expression of aerobic glycolysis-related genes and glycolysis, and ultimately leading to the suppression of liver cancer development.
    Mesh-Begriff(e) Humans ; Carcinoma, Hepatocellular/drug therapy ; Carcinoma, Hepatocellular/metabolism ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Protein p53/metabolism ; Reactive Oxygen Species/metabolism ; Liver Neoplasms/drug therapy ; Liver Neoplasms/metabolism ; Glycolysis ; Cell Proliferation ; Cell Line, Tumor ; Flavonoids
    Chemische Substanzen icaritin (UFE666UELY) ; Tumor Suppressor Protein p53 ; Reactive Oxygen Species ; Flavonoids
    Sprache Englisch
    Erscheinungsdatum 2024-03-01
    Erscheinungsland Ireland
    Dokumenttyp Journal Article
    ZDB-ID 218799-1
    ISSN 1872-7786 ; 0009-2797
    ISSN (online) 1872-7786
    ISSN 0009-2797
    DOI 10.1016/j.cbi.2024.110926
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  4. Artikel ; Online: Development and Clinical Validation of a Seven-Gene Prognostic Signature Based on Multiple Machine Learning Algorithms in Kidney Cancer

    Mi Tian / Tao Wang / Peng Wang

    Cell Transplantation, Vol

    2021  Band 30

    Abstract: About a third of patients with kidney cancer experience recurrence or cancer-related progression. Clinically, kidney cancer prognoses may be quite different, even in patients with kidney cancer at the same clinical stage. Therefore, there is an urgent ... ...

    Abstract About a third of patients with kidney cancer experience recurrence or cancer-related progression. Clinically, kidney cancer prognoses may be quite different, even in patients with kidney cancer at the same clinical stage. Therefore, there is an urgent need to screen for kidney cancer prognosis biomarkers. Differentially expressed genes (DEGs) were identified using kidney cancer RNA sequencing data from the Gene Expression Omnibus (GEO) database. Biomarkers were screened using random forest (RF) and support vector machine (SVM) models, and a multigene signature was constructed using the least absolute shrinkage and selection operator (LASSO) regression analysis. Univariate and multivariate Cox regression analyses were performed to explore the relationships between clinical features and prognosis. Finally, the reliability and clinical applicability of the model were validated, and relationships with biological pathways were identified. Western blots were also performed to evaluate gene expression. A total of 50 DEGs were obtained by intersecting the RF and SVM models. A seven-gene signature (RNASET2, EZH2, FXYD5, KIF18A, NAT8, CDCA7, and WNT7B) was constructed by LASSO regression. Univariate and multivariate Cox regression analyses showed that the seven-gene signature was an independent prognostic factor for kidney cancer. Finally, a predictive nomogram was established in The Cancer Genome Atlas (TCGA) cohort and validated internally. In tumor tissue, RNASET2 and FXYD5 were highly expressed and NAT8 was lowly expressed at the protein and transcription levels. This model could complement the clinicopathological characteristics of kidney cancer and promote the personalized management of patients with kidney cancer.
    Schlagwörter Medicine ; R
    Thema/Rubrik (Code) 616 ; 610
    Sprache Englisch
    Erscheinungsdatum 2021-02-01T00:00:00Z
    Verlag SAGE Publishing
    Dokumenttyp Artikel ; Online
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  5. Artikel ; Online: Relational Capital and Post-Traumatic Growth

    Ting Nie / Mi Tian / Hengrui Liang

    International Journal of Environmental Research and Public Health, Vol 18, Iss 7362, p

    The Role of Work Meaning

    2021  Band 7362

    Abstract: Through a statistical survey of 760 front-line medical staff during the COVID-19 epidemic, this study attempts to explore the relationships between relational capital, psychological security, post-traumatic growth and the meaning of work. Data analysis ... ...

    Abstract Through a statistical survey of 760 front-line medical staff during the COVID-19 epidemic, this study attempts to explore the relationships between relational capital, psychological security, post-traumatic growth and the meaning of work. Data analysis verifies that trust, reciprocity, and identification can promote post-traumatic growth by enhancing the individual’s psychological security. A high level of work meaning can enhance the role of trust, reciprocity and identification in promoting psychological security. Work meaning has a moderated mediating effect when trust and reciprocity affect post-traumatic growth through psychological security, but no moderated mediating effect is found when identification affects post-traumatic growth through psychological security.
    Schlagwörter relational capital ; post-traumatic growth ; psychological security ; work meaning ; Medicine ; R
    Sprache Englisch
    Erscheinungsdatum 2021-07-01T00:00:00Z
    Verlag MDPI AG
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  6. Artikel ; Online: miR-660-5p promotes breast cancer progression through down-regulating TET2 and activating PI3K/AKT/mTOR signaling

    Bing Peng / Chao Li / Lili He / Mi Tian / Xin Li

    Brazilian Journal of Medical and Biological Research, Vol 53, Iss

    2020  Band 12

    Abstract: Breast cancer (BC) is a commonly diagnosed cancer in females. MicroRNA-660-5p (miR-660-5p) has been reported to be involved in the occurrence and development of BC. However, the regulatory network of miR-660-5p in BC has not been fully addressed. ... ...

    Abstract Breast cancer (BC) is a commonly diagnosed cancer in females. MicroRNA-660-5p (miR-660-5p) has been reported to be involved in the occurrence and development of BC. However, the regulatory network of miR-660-5p in BC has not been fully addressed. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the enrichment of miR-660-5p and tet-eleven translocation 2 (TET2) in BC tissues and cells. Cell counting kit-8 (CCK8), flow cytometry, and transwell migration and invasion assays were used to measure cell proliferation, apoptosis, migration, and invasion. The target relationship between miR-660-5p and TET2 was confirmed by dual luciferase reporter assay. Protein expression was measured by western blot. The expression of miR-660-5p was elevated in BC, and high expression of miR-660-5p was closely related to lymph node metastasis, advanced TNM stage, and vascular invasion of BC tumors. miR-660-5p silencing inhibited cell proliferation and metastasis, but induced apoptosis of BC cells. TET2 was identified as a direct target of miR-660-5p, and the interference of TET2 partly reversed the suppressive effects of miR-660-5p silencing on the malignant potential of BC cells. miR-660-5p promoted BC progression partly through modulating TET2 and PI3K/AKT/mTOR signaling. miR-660-5p/TET2 axis might be a promising target for BC treatment.
    Schlagwörter Breast cancer ; miR-660-5p ; TET2 ; PI3K/AKT/mTOR signaling ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
    Thema/Rubrik (Code) 500 ; 616
    Sprache Englisch
    Erscheinungsdatum 2020-10-01T00:00:00Z
    Verlag Associação Brasileira de Divulgação Científica
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  7. Artikel ; Online: One-Year Follow-Up of Corneal Biomechanical Changes After Accelerated Transepithelial Corneal Cross-Linking in Pediatric Patients With Progressive Keratoconus

    Weijun Jian / Mi Tian / Xiaoyu Zhang / Ling Sun / Yang Shen / Meiyan Li / Xingtao Zhou

    Frontiers in Medicine, Vol

    2021  Band 8

    Abstract: Aims: This study aimed to investigate the corneal biomechanical changes and topographic outcomes of accelerated transepithelial corneal cross-linking (ATE-CXL) in pediatric progressive keratoconus.Methods: In this prospective longitudinal study, 31 eyes ... ...

    Abstract Aims: This study aimed to investigate the corneal biomechanical changes and topographic outcomes of accelerated transepithelial corneal cross-linking (ATE-CXL) in pediatric progressive keratoconus.Methods: In this prospective longitudinal study, 31 eyes of 28 pediatric patients with keratoconus (21 boys and 7 girls; mean age, 14.35 ± 2.68 years) undergoing ATE-CXL (epithelium-on procedure with 45 mW/cm2 for 320 s) were included. Corvis ST was used to measure dynamic corneal response parameters at baseline and at 12 month after ATE-CXL. Corneal keratometry and corneal thickness were measured using Pentacam pre-operatively and 1, 6, and 12 month post-operatively.Results: No serious complications occurred during or after ATE-CXL. The maximum keratometry values were 60.10 ± 7.51 D pre-operatively and 61.42 ± 8.92, 61.17 ± 7.96, and 60.02 ± 7.58 D at 1, 6, and 12 month after ATE-CXL (P > 0.05), respectively. Corneal thickness remained stable during the 12-month follow-up (P > 0.05). At post-operative 12 month, first applanation time (P < 0.001), first applanation length (P = 0.004), second applanation velocity (P = 0.014), highest concavity time (P = 0.022), and radius of curvature at highest concavity (P = 0.031) increased significantly. The value of stiffness parameter at first applanation was significantly increased from 57.70 ± 27.57 pre-operatively to 63.36 ± 27.09 at 12 months after ATE-CXL (P = 0.018).Conclusions: ATE-CXL is safe and effective in stabilizing the progression of pediatric keratoconus. Changes in corneal biomechanical response consistent with stiffening following ATE-CXL were observed in pediatric patients with keratoconus.
    Schlagwörter pediatric ; keratoconus ; accelerated transepithelial cross-linking ; Corvis ST ; corneal biomechanics ; Medicine (General) ; R5-920
    Sprache Englisch
    Erscheinungsdatum 2021-07-01T00:00:00Z
    Verlag Frontiers Media S.A.
    Dokumenttyp Artikel ; Online
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  8. Artikel ; Online: Low-Temperature-Mediated Promoter Methylation Relates to the Expression of

    Du, Jingjing / Wang, Junwei / Shan, Sicong / Mi, Tian / Song, Yulong / Xia, Yu / Ma, Shoucai / Zhang, Gaisheng / Ma, Lingjian / Niu, Na

    International journal of molecular sciences

    2023  Band 24, Heft 19

    Abstract: Chlorophyll is an indispensable photoreceptor in plant photosynthesis. Its anabolic imbalance is detrimental to individual growth and development. As an essential epigenetic modification, DNA methylation can induce phenotypic variations, such as leaf ... ...

    Abstract Chlorophyll is an indispensable photoreceptor in plant photosynthesis. Its anabolic imbalance is detrimental to individual growth and development. As an essential epigenetic modification, DNA methylation can induce phenotypic variations, such as leaf color transformation, by regulating gene expression. Albino line XN1376B is a natural mutation of winter wheat cultivar XN1376; however, the regulatory mechanism of its albinism is still unclear. In this study, we found that low temperatures induced albinism in XN1376B. The number of chloroplasts decreased as the phenomenon of bleaching intensified and the fence tissue and sponge tissue slowly dissolved. We identified six distinct
    Mesh-Begriff(e) Chlorophyll/metabolism ; Triticum/metabolism ; Temperature ; Photosynthesis/genetics ; DNA Methylation ; Arabidopsis/metabolism ; Albinism/genetics ; Albinism/metabolism ; Plant Leaves/metabolism
    Chemische Substanzen Chlorophyll (1406-65-1)
    Sprache Englisch
    Erscheinungsdatum 2023-09-28
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241914697
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  9. Artikel ; Online: Late-Onset Bartter Syndrome Type II Due to a Novel Compound Heterozygous Mutation in KCNJ1 Gene

    Mi Tian / Hui Peng / Xin Bi / Yan-Qiu Wang / Yong-Zhe Zhang / Yan Wu / Bei-Ru Zhang

    Frontiers in Medicine, Vol

    A Case Report and Literature Review

    2022  Band 9

    Abstract: BackgroundBartter syndrome (BS) type II is a rare autosomal recessive renal tubular disorder caused by mutations in the KCNJ1 gene, which encodes the apical renal outer medullary potassium (ROMK) channel in the thick ascending limb (TAL) of Henle’s loop. ...

    Abstract BackgroundBartter syndrome (BS) type II is a rare autosomal recessive renal tubular disorder caused by mutations in the KCNJ1 gene, which encodes the apical renal outer medullary potassium (ROMK) channel in the thick ascending limb (TAL) of Henle’s loop. BS type II is typically considered as a disorder of infancy and seldom seen in adults.Case PresentationA 34-year-old woman was admitted with generalized body numbness and hand convulsions, without growth retardation. Laboratory tests revealed hypokalemic metabolic alkalosis, hyperreninemic hyperaldosteronism, and nephrocalcinosis. She was misdiagnosed during the initial diagnosis process and was finally diagnosed with late-onset BS type II via genetic testing through next-generation sequencing combined with Sanger sequencing. A novel compound heterozygous p.Leu207Ile/p. Cys308Arg variant in exon 5 of the KCNJ1 gene from her parents was identified and speculated to be a potential pathogenic gene variation.ConclusionWe report a case of late-onset BS type II with a novel compound heterozygous mutation in KCNJ1. Both variants are novel and have never been reported. Our report will have a significant impact on the diagnosis of BS in other patients without typical clinical presentations and emphasizes the importance of genetic investigation.
    Schlagwörter Bartter syndrome type II ; KCNJ1 gene mutation ; nephrocalcinosis ; hypokalemia ; late onset ; Medicine (General) ; R5-920
    Thema/Rubrik (Code) 572
    Sprache Englisch
    Erscheinungsdatum 2022-04-01T00:00:00Z
    Verlag Frontiers Media S.A.
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  10. Artikel ; Online: Antitumor progenitor exhausted CD8

    Lan, Xin / Mi, Tian / Alli, Shanta / Guy, Cliff / Djekidel, Mohamed Nadhir / Liu, Xueyan / Boi, Shannon / Chowdhury, Partha / He, Minghong / Zehn, Dietmar / Feng, Yongqiang / Youngblood, Ben

    Nature immunology

    2024  Band 25, Heft 6, Seite(n) 1046–1058

    Abstract: The durability of an antitumor immune response is mediated in part by the persistence of progenitor exhausted ... ...

    Abstract The durability of an antitumor immune response is mediated in part by the persistence of progenitor exhausted CD8
    Mesh-Begriff(e) Animals ; CD8-Positive T-Lymphocytes/immunology ; Receptors, Antigen, T-Cell/metabolism ; Receptors, Antigen, T-Cell/immunology ; Mice ; Carcinoma, Lewis Lung/immunology ; Carcinoma, Lewis Lung/pathology ; Carcinoma, Lewis Lung/metabolism ; Mice, Inbred C57BL ; Hepatocyte Nuclear Factor 1-alpha/metabolism ; Cell Differentiation/immunology ; Dendritic Cells/immunology ; Signal Transduction/immunology ; Mice, Knockout ; Lymphocyte Activation/immunology ; Cell Self Renewal ; Mice, Transgenic ; Early Growth Response Protein 2
    Chemische Substanzen Receptors, Antigen, T-Cell ; Hepatocyte Nuclear Factor 1-alpha ; Hnf1a protein, mouse ; Egr2 protein, mouse ; Early Growth Response Protein 2
    Sprache Englisch
    Erscheinungsdatum 2024-05-30
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2016987-5
    ISSN 1529-2916 ; 1529-2908
    ISSN (online) 1529-2916
    ISSN 1529-2908
    DOI 10.1038/s41590-024-01843-8
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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