Artikel ; Online: Periostin-integrin interaction regulates force-induced TGF-β1 and α-SMA expression by hPDLSCs.
2023 Band 30, Heft 4, Seite(n) 2570–2579
Abstract: Objective: Periostin (PN), a major matricellular periodontal ligament (PDL) protein, modulates the remodeling of the PDL and bone, especially under mechanical stress. This study investigated the requirement of PN-integrin signaling in force-induced ... ...
| Abstract | Objective: Periostin (PN), a major matricellular periodontal ligament (PDL) protein, modulates the remodeling of the PDL and bone, especially under mechanical stress. This study investigated the requirement of PN-integrin signaling in force-induced expression of transforming growth factor-beta 1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) in human PDL stem cells (hPDLSCs). Methods: Cells were stimulated with intermittent compressive force (ICF) using computerized controlled apparatus. Cell migration was examined using in vitro scratch assay. The mRNA expression was examined using real-time polymerase chain reaction. The protein expression was determined using immunofluorescent staining and western blot analysis. Results: Stimulation with ICF for 24 h increased the expression of PN, TGF-β1, and α-SMA, along with increased SMAD2/3 phosphorylation. Knockdown of POSTN (PN gene) decreased the protein levels of TGF-β1 and pSMAD2/3 upon force stimulation. POSTN knockdown of hPDLSCs resulted in delayed cell migration, as determined by a scratch assay. However, migration improved after seeding these knockdown cells on pre-PN-coated surfaces. Further, the knockdown of αVβ5 significantly attenuated the force-induced TGF-β1 expression. Conclusion: Our findings indicate the importance of PN-αVβ5 interactions in ICF-induced TGF-β1 signaling and the expression of α-SMA. Findings support the critical role of PN in maintaining the PDL's tissue integrity and homeostasis. |
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| Mesh-Begriff(e) | Humans ; Cell Adhesion Molecules/metabolism ; Cell Adhesion Molecules/genetics ; Transforming Growth Factor beta1/metabolism ; Actins/metabolism ; Cell Movement ; Periodontal Ligament/cytology ; Periodontal Ligament/metabolism ; Stem Cells/metabolism ; Cells, Cultured ; Stress, Mechanical ; Smad3 Protein/metabolism ; Smad2 Protein/metabolism ; Signal Transduction ; Periostin | |||||
| Chemische Substanzen | POSTN protein, human ; Cell Adhesion Molecules ; Transforming Growth Factor beta1 ; Actins ; ACTA2 protein, human ; Smad3 Protein ; Smad2 Protein ; SMAD3 protein, human ; SMAD2 protein, human ; Periostin | |||||
| Sprache | Englisch | |||||
| Erscheinungsdatum | 2023-07-19 | |||||
| Erscheinungsland | Denmark | |||||
| Dokumenttyp | Journal Article | |||||
| ZDB-ID | 1290529-x | |||||
| ISSN | 1601-0825 ; 1354-523X | |||||
| ISSN (online) | 1601-0825 | |||||
| ISSN | 1354-523X | |||||
| DOI | 10.1111/odi.14691 | |||||
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| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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