Article ; Online: The exon junction complex core factor eIF4A3 is a key regulator of HPV16 gene expression.
2021 Volume 41, Issue 4
Abstract: High-risk human papillomavirus (hrHPVs), particularly HPV16 and HPV18, are the etiologic factors of ano-genital cancers and some head and neck squamous cell carcinomas (HNSCCs). Viral E6 and E7 oncoproteins, controlled at both transcriptional and post- ... ...
Abstract | High-risk human papillomavirus (hrHPVs), particularly HPV16 and HPV18, are the etiologic factors of ano-genital cancers and some head and neck squamous cell carcinomas (HNSCCs). Viral E6 and E7 oncoproteins, controlled at both transcriptional and post-transcriptional levels, drive hrHPVs-induced carcinogenesis. In the present study, we investigated the implication of the DEAD-box helicase eukaryotic translation initiation factor 4A3 (eIF4A3,) an Exon Junction Complex factor, in the regulation of HPV16 gene expression. Our data revealed that the depletion of the factor eIF4A3 up-regulated E7 oncoprotein levels. We also showed that the inhibition of the nonsense-mediated RNA decay (NMD) pathway, resulted in the up-regulation of E7 at both RNA and protein levels. We therefore proposed that HPV16 transcripts might present different susceptibilities to NMD and that this pathway could play a key role in the levels of expression of these viral oncoproteins during the development of HPV-related cancers. |
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MeSH term(s) | Cell Line, Tumor ; DEAD-box RNA Helicases/metabolism ; Eukaryotic Initiation Factor-4A/metabolism ; Host-Pathogen Interactions ; Human papillomavirus 16/genetics ; Human papillomavirus 16/metabolism ; Humans ; Papillomavirus E7 Proteins/genetics ; Papillomavirus E7 Proteins/metabolism |
Chemical Substances | Papillomavirus E7 Proteins ; oncogene protein E7, Human papillomavirus type 16 ; Eukaryotic Initiation Factor-4A (EC 2.7.7.-) ; EIF4A3 protein, human (EC 3.6.1.-) ; DEAD-box RNA Helicases (EC 3.6.4.13) |
Language | English |
Publishing date | 2021-03-24 |
Publishing country | England |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 764946-0 |
ISSN | 1573-4935 ; 0144-8463 |
ISSN (online) | 1573-4935 |
ISSN | 0144-8463 |
DOI | 10.1042/BSR20203488 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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