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  1. Article ; Online: Combining Fluorescent In Situ Hybridization with Immunofluorescence and Lectin Staining in Planarians.

    Font-Martín, Daniel / Pascual-Carreras, Eudald / Saló, Emili

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2680, Page(s) 67–79

    Abstract: The capability to simultaneously apply different molecular tools to visualize a wide variety of changes in genetic expression and tissue composition in Schmidtea mediterranea has always been of great interest. The most commonly used techniques are ... ...

    Abstract The capability to simultaneously apply different molecular tools to visualize a wide variety of changes in genetic expression and tissue composition in Schmidtea mediterranea has always been of great interest. The most commonly used techniques are fluorescent in situ hybridization (FISH) and immunofluorescence (IF) detection. Here, we describe a novel way to perform both protocols together adding the possibility to combine them with fluorescent-conjugated lectin staining to further broaden the detection of tissues. We also present a novel lectin fixation protocol to enhance the signal, which could be useful when single-cell resolution is required.
    MeSH term(s) Animals ; In Situ Hybridization, Fluorescence ; Planarians/genetics ; Lectins/genetics ; Lectins/metabolism ; Fluorescent Antibody Technique ; Gene Expression
    Chemical Substances Lectins
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3275-8_4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: FoxK1

    Coronel-Córdoba, Pablo / Molina, M Dolores / Cardona, Gemma / Fraguas, Susanna / Pascual-Carreras, Eudald / Saló, Emili / Cebrià, Francesc / Adell, Teresa

    Frontiers in cell and developmental biology

    2022  Volume 10, Page(s) 808045

    Abstract: Forkhead box (Fox) genes belong to the "winged helix" transcription factor superfamily. The function of some Fox genes is well known, such as the role ... ...

    Abstract Forkhead box (Fox) genes belong to the "winged helix" transcription factor superfamily. The function of some Fox genes is well known, such as the role of
    Language English
    Publishing date 2022-02-22
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2737824-X
    ISSN 2296-634X
    ISSN 2296-634X
    DOI 10.3389/fcell.2022.808045
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Wnt/β-catenin signalling is required for pole-specific chromatin remodeling during planarian regeneration.

    Pascual-Carreras, Eudald / Marín-Barba, Marta / Castillo-Lara, Sergio / Coronel-Córdoba, Pablo / Magri, Marta Silvia / Wheeler, Grant N / Gómez-Skarmeta, Jose Luis / Abril, Josep F / Saló, Emili / Adell, Teresa

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 298

    Abstract: For successful regeneration, the identity of the missing tissue must be specified according to the pre-existing tissue. Planarians are ideal for the study of the mechanisms underlying this process; the same field of cells can regrow a head or a tail ... ...

    Abstract For successful regeneration, the identity of the missing tissue must be specified according to the pre-existing tissue. Planarians are ideal for the study of the mechanisms underlying this process; the same field of cells can regrow a head or a tail according to the missing body part. After amputation, the differential activation of the Wnt/β-catenin signal specifies anterior versus posterior identity. Initially, both wnt1 and notum (Wnt inhibitor) are expressed in all wounds, but 48 hours later they are restricted to posterior or anterior facing wounds, respectively, by an unknown mechanism. Here we show that 12 hours after amputation, the chromatin accessibility of cells in the wound region changes according to the polarity of the pre-existing tissue in a Wnt/β-catenin-dependent manner. Genomic analyses suggest that homeobox transcription factors and chromatin-remodeling proteins are direct Wnt/β-catenin targets, which trigger the expression of posterior effectors. Finally, we identify FoxG as a wnt1 up-stream regulator, probably via binding to its first intron enhancer region.
    MeSH term(s) Animals ; Planarians/physiology ; Wnt Proteins/genetics ; Wnt Proteins/metabolism ; Chromatin Assembly and Disassembly ; beta Catenin/genetics ; beta Catenin/metabolism ; Body Patterning/genetics
    Chemical Substances Wnt Proteins ; beta Catenin
    Language English
    Publishing date 2023-01-18
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-35937-y
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: WNT-FRIZZLED-LRP5/6 Signaling Mediates Posterior Fate and Proliferation during Planarian Regeneration.

    Pascual-Carreras, Eudald / Sureda-Gómez, Miquel / Barrull-Mascaró, Ramon / Jordà, Natàlia / Gelabert, Maria / Coronel-Córdoba, Pablo / Saló, Emili / Adell, Teresa

    Genes

    2021  Volume 12, Issue 1

    Abstract: An organizer is defined as a group of cells that secrete extracellular proteins that specify the fate of surrounding cells according to their concentration. Their function during embryogenesis is key in patterning new growing tissues. Although organizers ...

    Abstract An organizer is defined as a group of cells that secrete extracellular proteins that specify the fate of surrounding cells according to their concentration. Their function during embryogenesis is key in patterning new growing tissues. Although organizers should also participate in adult development when new structures are regenerated, their presence in adults has only been identified in a few species with striking regenerative abilities, such as planarians. Planarians provide a unique model to understand the function of adult organizers, since the presence of adult pluripotent stem cells provides them with the ability to regenerate any body part. Previous studies have shown that the differential activation of the WNT/β-catenin signal in each wound is fundamental to establish an anterior or a posterior organizer in the corresponding wound. Here, we identify the receptors that mediate the WNT/β-catenin signal in posterior-facing wounds. We found that Wnt1-Fzd1-LRP5/6 signaling is evolutionarily conserved in executing a WNT/β-catenin signal to specify cell fate and to trigger a proliferative response. Our data allow a better understanding of the mechanism through which organizers signal to a "competent" field of cells and integrate the patterning and growth required during de novo formation of organs and tissues.
    MeSH term(s) Animals ; Cell Proliferation/physiology ; Frizzled Receptors/metabolism ; Helminth Proteins/metabolism ; LDL-Receptor Related Proteins/metabolism ; Planarians/physiology ; Regeneration/physiology ; Wnt Signaling Pathway/physiology
    Chemical Substances Frizzled Receptors ; Helminth Proteins ; LDL-Receptor Related Proteins
    Language English
    Publishing date 2021-01-15
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12010101
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Analysis of Fox genes in Schmidtea mediterranea reveals new families and a conserved role of Smed-foxO in controlling cell death.

    Pascual-Carreras, Eudald / Herrera-Úbeda, Carlos / Rosselló, Maria / Coronel-Córdoba, Pablo / Garcia-Fernàndez, Jordi / Saló, Emili / Adell, Teresa

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 2947

    Abstract: The forkhead box (Fox) genes encode transcription factors that control several key aspects of development. Present in the ancestor of all eukaryotes, Fox genes underwent several duplications followed by loss and diversification events that gave rise to ... ...

    Abstract The forkhead box (Fox) genes encode transcription factors that control several key aspects of development. Present in the ancestor of all eukaryotes, Fox genes underwent several duplications followed by loss and diversification events that gave rise to the current 25 families. However, few Fox members have been identified from the Lophotrochozoa clade, and specifically from planarians, which are a unique model for understanding development, due to the striking plasticity of the adult. The aim of this study was to identify and perform evolutionary and functional studies of the Fox genes of lophotrochozoan species and, specifically, of the planarian Schmidtea mediterranea. Generating a pipeline for identifying Forkhead domains and using phylogenetics allowed us the phylogenetic reconstruction of Fox genes. We corrected the annotation for misannotated genes and uncovered a new family, the QD, present in all metazoans. According to the new phylogeny, the 27 Fox genes found in Schmidtea mediterranea were classified into 12 families. In Platyhelminthes, family losses were accompanied by extensive gene diversification and the appearance of specific families, the A(P) and N(P). Among the newly identified planarian Fox genes, we found a single copy of foxO, which shows an evolutionary conserved role in controlling cell death.
    MeSH term(s) Animals ; Biological Evolution ; Forkhead Transcription Factors/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Helminth Proteins/metabolism ; Phylogeny ; Planarians/genetics ; Regulated Cell Death/genetics
    Chemical Substances Forkhead Transcription Factors ; Helminth Proteins
    Language English
    Publishing date 2021-02-03
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-020-80627-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: WNT-FRIZZLED-LRP5/6 Signaling Mediates Posterior Fate and Proliferation during Planarian Regeneration

    Pascual-Carreras, Eudald / Sureda-Gómez, Miquel / Barrull-Mascaró, Ramon / Jordà, Natàlia / Gelabert, Maria / Coronel-Córdoba, Pablo / Saló, Emili / Adell, Teresa

    Genes. 2021 Jan. 15, v. 12, no. 1

    2021  

    Abstract: An organizer is defined as a group of cells that secrete extracellular proteins that specify the fate of surrounding cells according to their concentration. Their function during embryogenesis is key in patterning new growing tissues. Although organizers ...

    Abstract An organizer is defined as a group of cells that secrete extracellular proteins that specify the fate of surrounding cells according to their concentration. Their function during embryogenesis is key in patterning new growing tissues. Although organizers should also participate in adult development when new structures are regenerated, their presence in adults has only been identified in a few species with striking regenerative abilities, such as planarians. Planarians provide a unique model to understand the function of adult organizers, since the presence of adult pluripotent stem cells provides them with the ability to regenerate any body part. Previous studies have shown that the differential activation of the WNT/β-catenin signal in each wound is fundamental to establish an anterior or a posterior organizer in the corresponding wound. Here, we identify the receptors that mediate the WNT/β-catenin signal in posterior-facing wounds. We found that Wnt1-Fzd1-LRP5/6 signaling is evolutionarily conserved in executing a WNT/β-catenin signal to specify cell fate and to trigger a proliferative response. Our data allow a better understanding of the mechanism through which organizers signal to a “competent” field of cells and integrate the patterning and growth required during de novo formation of organs and tissues.
    Keywords adult development ; adults ; embryogenesis ; models
    Language English
    Dates of publication 2021-0115
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12010101
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Posterior Wnts Have Distinct Roles in Specification and Patterning of the Planarian Posterior Region.

    Sureda-Gómez, Miquel / Pascual-Carreras, Eudald / Adell, Teresa

    International journal of molecular sciences

    2015  Volume 16, Issue 11, Page(s) 26543–26554

    Abstract: The wnt signaling pathway is an intercellular communication mechanism essential in cell-fate specification, tissue patterning and regional-identity specification. A βcatenin-dependent signal specifies the AP (Anteroposterior) axis of planarians, both ... ...

    Abstract The wnt signaling pathway is an intercellular communication mechanism essential in cell-fate specification, tissue patterning and regional-identity specification. A βcatenin-dependent signal specifies the AP (Anteroposterior) axis of planarians, both during regeneration of new tissues and during normal homeostasis. Accordingly, four wnts (posterior wnts) are expressed in a nested manner in central and posterior regions of planarians. We have analyzed the specific role of each posterior wnt and the possible cooperation between them in specifying and patterning planarian central and posterior regions. We show that each posterior wnt exerts a distinct role during re-specification and maintenance of the central and posterior planarian regions, and that the integration of the different wnt signals (βcatenin dependent and independent) underlies the patterning of the AP axis from the central region to the tip of the tail. Based on these findings and data from the literature, we propose a model for patterning the planarian AP axis.
    MeSH term(s) Animals ; Body Patterning/genetics ; Gene Silencing ; Homeostasis ; Planarians/genetics ; Planarians/growth & development ; Planarians/metabolism ; Regeneration/genetics ; Wnt Proteins/genetics ; Wnt Proteins/metabolism ; Wnt Signaling Pathway
    Chemical Substances Wnt Proteins
    Language English
    Publishing date 2015-11-05
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms161125970
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Planarian cell number depends on

    Pascual-Carreras, Eudald / Marin-Barba, Marta / Herrera-Úbeda, Carlos / Font-Martín, Daniel / Eckelt, Kay / de Sousa, Nidia / García-Fernández, Jordi / Saló, Emili / Adell, Teresa

    Development (Cambridge, England)

    2020  Volume 147, Issue 7

    Abstract: Control of cell number is crucial to define body size during animal development and to restrict tumoral transformation. The cell number is determined by the balance between cell proliferation and cell death. Although many genes are known to regulate ... ...

    Abstract Control of cell number is crucial to define body size during animal development and to restrict tumoral transformation. The cell number is determined by the balance between cell proliferation and cell death. Although many genes are known to regulate those processes, the molecular mechanisms underlying the relationship between cell number and body size remain poorly understood. This relationship can be better understood by studying planarians, flatworms that continuously change their body size according to nutrient availability. We identified a novel gene family,
    MeSH term(s) Animals ; Animals, Genetically Modified ; Apoptosis Regulatory Proteins/genetics ; Apoptosis Regulatory Proteins/metabolism ; Apoptosis Regulatory Proteins/physiology ; Cell Count ; Cell Death/genetics ; Cell Proliferation/genetics ; Chromosome Mapping ; Gene Expression Regulation, Developmental ; Homeostasis/genetics ; Multigene Family/physiology ; Planarians/classification ; Planarians/cytology ; Planarians/genetics ; Planarians/physiology ; Regeneration/genetics ; Tandem Repeat Sequences
    Chemical Substances Apoptosis Regulatory Proteins
    Language English
    Publishing date 2020-04-08
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.184044
    Database MEDical Literature Analysis and Retrieval System OnLINE

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