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  1. Artikel ; Online: Disease-Associated Mutations in Tau Encode for Changes in Aggregate Structure Conformation.

    Sun, Kerry T / Patel, Tark / Kang, Sang-Gyun / Yarahmady, Allan / Srinivasan, Mahalashmi / Julien, Olivier / Heras, Jónathan / Mok, Sue-Ann

    ACS chemical neuroscience

    2023  Band 14, Heft 24, Seite(n) 4282–4297

    Abstract: The accumulation of tau fibrils is associated with neurodegenerative diseases, which are collectively termed tauopathies. Cryo-EM studies have shown that the packed fibril core of tau adopts distinct structures in different tauopathies, such as Alzheimer' ...

    Abstract The accumulation of tau fibrils is associated with neurodegenerative diseases, which are collectively termed tauopathies. Cryo-EM studies have shown that the packed fibril core of tau adopts distinct structures in different tauopathies, such as Alzheimer's disease, corticobasal degeneration, and progressive supranuclear palsy. A subset of tauopathies are linked to missense mutations in the tau protein, but it is not clear whether these mutations impact the structure of tau fibrils. To answer this question, we developed a high-throughput protein purification platform and purified a panel of 37 tau variants using the full-length 0N4R splice isoform. Each of these variants was used to create fibrils
    Mesh-Begriff(e) Humans ; tau Proteins/metabolism ; Tauopathies/metabolism ; Alzheimer Disease/metabolism ; Mutation/genetics
    Chemische Substanzen tau Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-12-06
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 1948-7193
    ISSN (online) 1948-7193
    DOI 10.1021/acschemneuro.3c00422
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Native PLGA nanoparticles attenuate Aβ-seed induced tau aggregation under in vitro conditions: potential implication in Alzheimer's disease pathology.

    Paul, Pallabi Sil / Patel, Tark / Cho, Jae-Young / Yarahmady, Allan / Khalili, Aria / Semenchenko, Valentyna / Wille, Holger / Kulka, Marianna / Mok, Sue-Ann / Kar, Satyabrata

    Scientific reports

    2024  Band 14, Heft 1, Seite(n) 144

    Abstract: Evidence suggests that beta-amyloid (Aβ)-induced phosphorylation/aggregation of tau protein plays a critical role in the degeneration of neurons and development of Alzheimer's disease (AD), the most common cause of dementia affecting the elderly ... ...

    Abstract Evidence suggests that beta-amyloid (Aβ)-induced phosphorylation/aggregation of tau protein plays a critical role in the degeneration of neurons and development of Alzheimer's disease (AD), the most common cause of dementia affecting the elderly population. Many studies have pursued a variety of small molecules, including nanoparticles conjugated with drugs to interfere with Aβ and/or tau aggregation/toxicity as an effective strategy for AD treatment. We reported earlier that FDA approved PLGA nanoparticles without any drug can attenuate Aβ aggregation/toxicity in cellular/animal models of AD. In this study, we evaluated the effects of native PLGA on Aβ seed-induced aggregation of tau protein using a variety of biophysical, structural and spectroscopic approaches. Our results show that Aβ
    Mesh-Begriff(e) Aged ; Animals ; Humans ; Alzheimer Disease/metabolism ; tau Proteins/metabolism ; Amyloid beta-Peptides/metabolism ; Phosphorylation ; Nanoparticles
    Chemische Substanzen tau Proteins ; Amyloid beta-Peptides
    Sprache Englisch
    Erscheinungsdatum 2024-01-02
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-50465-x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Streamlined high-throughput cloning protocol to generate arrayed mutant libraries.

    Sun, Kerry T / Patel, Tark S / Kim, Justin / Tang, Helen S H / Eskandari-Sedighi, Ghazaleh / Sureshkumar, Haresh / Schieve, Dean / Mok, S A

    STAR protocols

    2022  Band 4, Heft 1, Seite(n) 101930

    Abstract: Large-scale, site-directed mutagenesis enables rapid characterization of the biochemical and biological properties of proteins. Here, we present a cost-effective and adaptable cloning pipeline to generate arrayed gene libraries for a construct of ... ...

    Abstract Large-scale, site-directed mutagenesis enables rapid characterization of the biochemical and biological properties of proteins. Here, we present a cost-effective and adaptable cloning pipeline to generate arrayed gene libraries for a construct of interest. We detail steps to use an open access web app to automate the design of mutagenesis primers optimized for our cloning protocols in a 96-well plate format. The protocol allows most molecular biology labs to clone 96 mutants (from PCR to sequence ready plasmid) in 3 days.
    Mesh-Begriff(e) Polymerase Chain Reaction/methods ; Gene Library ; Mutagenesis ; Mutagenesis, Site-Directed ; Cloning, Molecular
    Sprache Englisch
    Erscheinungsdatum 2022-12-13
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2666-1667
    ISSN (online) 2666-1667
    DOI 10.1016/j.xpro.2022.101930
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: APOE3-R136S

    Naguib, Sarah / Torres, Eileen Ruth / Lopez-Lee, Chloe / Fan, Li / Bhagwat, Maitreyee / Norman, Kendra / Lee, Se-In / Zhu, Jingjie / Ye, Pearly / Wong, Man Ying / Patel, Tark / Mok, Sue-Ann / Luo, Wenjie / Sinha, Subhash / Zhao, Mingrui / Gong, Shiaoching / Gan, Li

    bioRxiv : the preprint server for biology

    2024  

    Abstract: The Christchurch mutation (R136S) on ... ...

    Abstract The Christchurch mutation (R136S) on the
    Sprache Englisch
    Erscheinungsdatum 2024-04-28
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2024.04.25.591140
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: DAP12 deficiency alters microglia-oligodendrocyte communication and enhances resilience against tau toxicity.

    Chen, Hao / Fan, Li / Guo, Qi / Wong, Man Ying / Yu, Fangmin / Foxe, Nessa / Wang, Winston / Nessim, Aviram / Carling, Gillian / Liu, Bangyan / Lopez-Lee, Chloe / Huang, Yige / Amin, Sadaf / Patel, Tark / Mok, Sue-Ann / Song, Won-Min / Zhang, Bin / Ma, Qin / Fu, Hongjun /
    Gan, Li / Luo, Wenjie

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Pathogenic tau accumulation fuels neurodegeneration in Alzheimer's disease (AD). Enhancing aging brain's resilience to tau pathology would lead to novel therapeutic strategies. DAP12 (DNAX-activation protein 12) is critically involved in microglial ... ...

    Abstract Pathogenic tau accumulation fuels neurodegeneration in Alzheimer's disease (AD). Enhancing aging brain's resilience to tau pathology would lead to novel therapeutic strategies. DAP12 (DNAX-activation protein 12) is critically involved in microglial immune responses. Previous studies have showed that mice lacking DAP12 in tauopathy mice exhibit higher tau pathology but are protected from tau-induced cognitive deficits. However, the exact mechanism remains elusive. Our current study uncovers a novel resilience mechanism via microglial interaction with oligodendrocytes. Despite higher tau inclusions, Dap12 deletion curbs tau-induced brain inflammation and ameliorates myelin and synapse loss. Specifically, removal of Dap12 abolished tau-induced disease-associated clusters in microglia (MG) and intermediate oligodendrocytes (iOli), which are spatially correlated with tau pathology in AD brains. Our study highlights the critical role of interactions between microglia and oligodendrocytes in tau toxicity and DAP12 signaling as a promising target for enhancing resilience in AD.
    Sprache Englisch
    Erscheinungsdatum 2023-10-28
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.10.26.563970
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.

    Bravo, Celeste Parra / Giani, Alice Maria / Perez, Jesus Madero / Zhao, Zeping / Samelson, Avi / Wong, Man Ying / Evangelisti, Alessandro / Fan, Li / Pozner, Tatyana / Mercedes, Maria / Ye, Pearly / Patel, Tark / Yarahmady, Allan / Carling, Gillian / Lee, Virginia M Y / Sharma, Manu / Mok, Sue-Ann / Luo, Wenjie / Zhao, Mingrui /
    Kampmann, Martin / Gong, Shiaoching / Gan, Li

    bioRxiv : the preprint server for biology

    2023  

    Abstract: Tauopathies are age-associated neurodegenerative diseases whose mechanistic underpinnings remain elusive, partially due to lack of appropriate human models. Current human induced pluripotent stem cell (hiPSC)-derived neurons express very low levels of 4- ... ...

    Abstract Tauopathies are age-associated neurodegenerative diseases whose mechanistic underpinnings remain elusive, partially due to lack of appropriate human models. Current human induced pluripotent stem cell (hiPSC)-derived neurons express very low levels of 4-repeat (4R)-tau isoforms that are normally expressed in adult brain. Here, we engineered new iPSC lines to express 4R-tau and 4R-tau carrying the P301S
    Sprache Englisch
    Erscheinungsdatum 2023-06-22
    Erscheinungsland United States
    Dokumenttyp Preprint
    DOI 10.1101/2023.06.19.544278
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  7. Artikel ; Online: Human iPSC 4R tauopathy model uncovers modifiers of tau propagation.

    Parra Bravo, Celeste / Giani, Alice Maria / Madero-Perez, Jesus / Zhao, Zeping / Wan, Yuansong / Samelson, Avi J / Wong, Man Ying / Evangelisti, Alessandro / Cordes, Ethan / Fan, Li / Ye, Pearly / Zhu, Daphne / Pozner, Tatyana / Mercedes, Maria / Patel, Tark / Yarahmady, Allan / Carling, Gillian K / Sterky, Fredrik H / Lee, Virginia M Y /
    Lee, Edward B / DeTure, Michael / Dickson, Dennis W / Sharma, Manu / Mok, Sue-Ann / Luo, Wenjie / Zhao, Mingrui / Kampmann, Martin / Gong, Shiaoching / Gan, Li

    Cell

    2024  Band 187, Heft 10, Seite(n) 2446–2464.e22

    Abstract: Tauopathies are age-associated neurodegenerative diseases whose mechanistic underpinnings remain elusive, partially due to a lack of appropriate human models. Here, we engineered human induced pluripotent stem cell (hiPSC)-derived neuronal lines to ... ...

    Abstract Tauopathies are age-associated neurodegenerative diseases whose mechanistic underpinnings remain elusive, partially due to a lack of appropriate human models. Here, we engineered human induced pluripotent stem cell (hiPSC)-derived neuronal lines to express 4R Tau and 4R Tau carrying the P301S MAPT mutation when differentiated into neurons. 4R-P301S neurons display progressive Tau inclusions upon seeding with Tau fibrils and recapitulate features of tauopathy phenotypes including shared transcriptomic signatures, autophagic body accumulation, and reduced neuronal activity. A CRISPRi screen of genes associated with Tau pathobiology identified over 500 genetic modifiers of seeding-induced Tau propagation, including retromer VPS29 and genes in the UFMylation cascade. In progressive supranuclear palsy (PSP) and Alzheimer's Disease (AD) brains, the UFMylation cascade is altered in neurofibrillary-tangle-bearing neurons. Inhibiting the UFMylation cascade in vitro and in vivo suppressed seeding-induced Tau propagation. This model provides a robust platform to identify novel therapeutic strategies for 4R tauopathy.
    Mesh-Begriff(e) Humans ; Induced Pluripotent Stem Cells/metabolism ; tau Proteins/metabolism ; Tauopathies/metabolism ; Tauopathies/pathology ; Neurons/metabolism ; Neurons/pathology ; Animals ; Mice ; Alzheimer Disease/metabolism ; Alzheimer Disease/pathology ; Alzheimer Disease/genetics ; Brain/metabolism ; Brain/pathology ; Supranuclear Palsy, Progressive/metabolism ; Supranuclear Palsy, Progressive/pathology ; Supranuclear Palsy, Progressive/genetics ; Cell Differentiation ; Mutation ; Autophagy
    Chemische Substanzen tau Proteins ; MAPT protein, human
    Sprache Englisch
    Erscheinungsdatum 2024-04-05
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2024.03.015
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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