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Artikel ; Online: Retrieval and purification of human β cells from stem cell-derived islet engrafted mice.

Kenty, Jennifer Hyoje-Ryu / Pelayo, Gabriela Cristina / Leite, Nayara Carvalho

STAR protocols

2021  Band 2, Heft 3, Seite(n) 100675

Abstract: Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a ... ...

Abstract Stem cell-derived beta cells (SC-β-cells) engrafted into mice serve as a pre-clinical model of diabetes. It is helpful to recover viable β cells following transplantation to perform tests on the graft. We developed a protocol to retrieve and purify a sufficient number of live β cells from mice following long-term human SC-β-cell engraftment. The protocol enables examination of SC-β-cells undergoing developmental and metabolic changes
Mesh-Begriff(e) Animals ; Cell Separation/methods ; Heterografts ; Humans ; Insulin-Secreting Cells/cytology ; Insulin-Secreting Cells/transplantation ; Islets of Langerhans Transplantation/methods ; Mice ; Stem Cells/cytology
Sprache Englisch
Erscheinungsdatum 2021-07-15
Erscheinungsland United States
Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
ISSN 2666-1667
ISSN (online) 2666-1667
DOI 10.1016/j.xpro.2021.100675
Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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