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  1. Artikel ; Online: ROS signaling in innate immunity via oxidative protein modifications.

    Manoharan, Renuka Ramalingam / Prasad, Ankush / Pospíšil, Pavel / Kzhyshkowska, Julia

    Frontiers in immunology

    2024  Band 15, Seite(n) 1359600

    Abstract: The innate immune response represents the first-line of defense against invading pathogens. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been implicated in various aspects of innate immune function, which involves respiratory ... ...

    Abstract The innate immune response represents the first-line of defense against invading pathogens. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) have been implicated in various aspects of innate immune function, which involves respiratory bursts and inflammasome activation. These reactive species widely distributed within the cellular environment are short-lived intermediates that play a vital role in cellular signaling and proliferation and are likely to depend on their subcellular site of formation. NADPH oxidase complex of phagocytes is known to generate superoxide anion radical (O
    Mesh-Begriff(e) Hydrogen Peroxide ; Oxidation-Reduction ; Superoxides ; Oxidative Stress ; Hypochlorous Acid ; Immunity, Innate
    Chemische Substanzen Hydrogen Peroxide (BBX060AN9V) ; Superoxides (11062-77-4) ; Hypochlorous Acid (712K4CDC10)
    Sprache Englisch
    Erscheinungsdatum 2024-03-07
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2606827-8
    ISSN 1664-3224 ; 1664-3224
    ISSN (online) 1664-3224
    ISSN 1664-3224
    DOI 10.3389/fimmu.2024.1359600
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Malondialdehyde enhances PsbP protein release during heat stress in Arabidopsis.

    Kumar, Aditya / Prasad, Ankush / Sedlářová, Michaela / Pospíšil, Pavel

    Plant physiology and biochemistry : PPB

    2023  Band 202, Seite(n) 107984

    Abstract: Under environmental conditions, plants are exposed to various abiotic and biotic stress factors, which commonly cause the oxidation of lipids and proteins. Lipid peroxidation constantly produces malondialdehyde (MDA), a secondary product of lipid ... ...

    Abstract Under environmental conditions, plants are exposed to various abiotic and biotic stress factors, which commonly cause the oxidation of lipids and proteins. Lipid peroxidation constantly produces malondialdehyde (MDA), a secondary product of lipid peroxidation, which is covalently bound to proteins forming MDA-protein adducts. The spatial distribution of MDA-protein adducts in Arabidopsis leaves shows that MDA-protein adducts are located in the chloroplasts, uniformly spread out over the thylakoid membrane. At the lumenal side of thylakoid membrane, MDA interacts with PsbP, an extrinsic subunit of the photosystem II (PSII), which is in electrostatic interaction with the PSII core proteins. Under heat stress, when MDA is moderately enhanced, the electrostatic interaction between PsbP and PSII core proteins is weakened, and PsbP with bound MDA is released in the lumen. It is proposed here that the electrophilic MDA is bound to the nucleophilic lysine residues of PsbP, which are involved in electrostatic interactions with the negatively charged glutamate of the PSII core protein. Our data provide crucial information about the MDA binding topology in the higher plant PSII complex, which is necessary to understand better the physiological functions of MDA for plant survival under stress.
    Mesh-Begriff(e) Malondialdehyde ; Arabidopsis ; Glutamic Acid ; Lysine ; Heat-Shock Response
    Chemische Substanzen Malondialdehyde (4Y8F71G49Q) ; Glutamic Acid (3KX376GY7L) ; Lysine (K3Z4F929H6)
    Sprache Englisch
    Erscheinungsdatum 2023-08-23
    Erscheinungsland France
    Dokumenttyp Journal Article
    ZDB-ID 742978-2
    ISSN 1873-2690 ; 0981-9428
    ISSN (online) 1873-2690
    ISSN 0981-9428
    DOI 10.1016/j.plaphy.2023.107984
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  3. Artikel ; Online: Oxidative modification of collagen by malondialdehyde in porcine skin.

    Paculová, Vendula / Prasad, Ankush / Sedlářová, Michaela / Pospíšil, Pavel

    Archives of biochemistry and biophysics

    2023  Band 752, Seite(n) 109850

    Abstract: Human skin is exposed to various physical and chemical stress factors, which commonly cause the oxidation of lipids and proteins. In this study, azo initiator AAPH [2,2' -azobis(2-methylpropionamidine) dihydrochloride] was employed to initiate lipid ... ...

    Abstract Human skin is exposed to various physical and chemical stress factors, which commonly cause the oxidation of lipids and proteins. In this study, azo initiator AAPH [2,2' -azobis(2-methylpropionamidine) dihydrochloride] was employed to initiate lipid peroxidation in porcine skin as an ex vivo model for human skin. We demonstrate that malondialdehyde (MDA), a secondary product of lipid peroxidation, is covalently bound to collagen in the dermis, forming MDA-collagen adducts. The binding of MDA to collagen results in an unfolding of the collagen triple helix, formation of the dimer of α-chains of collagen, and fragmentation of the collagen α-chain. It is proposed here that the MDA is bound to the lysine residues of α-chain collagen, which are involved in electrostatic interaction and hydrogen bonding with the glutamate and aspartate of other α-chains of the triple helix. Our data provide crucial information about the MDA binding topology in the skin, which is necessary to understand better the various types of skin-related diseases and the aging process in the skin under stress.
    Mesh-Begriff(e) Humans ; Swine ; Malondialdehyde/metabolism ; Oxidation-Reduction ; Collagen/metabolism ; Lipid Peroxidation ; Oxidative Stress ; Animals
    Chemische Substanzen Malondialdehyde (4Y8F71G49Q) ; Collagen (9007-34-5)
    Sprache Englisch
    Erscheinungsdatum 2023-12-06
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 523-x
    ISSN 1096-0384 ; 0003-9861
    ISSN (online) 1096-0384
    ISSN 0003-9861
    DOI 10.1016/j.abb.2023.109850
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  4. Artikel ; Online: Reactive oxygen species in photosystem II: relevance for oxidative signaling

    Pospisil, Pavel / Kumar, Aditya / Prasad, Ankush

    Photosynth Res. 2022 June, v. 152, no. 3 p.245-260

    2022  

    Abstract: Reactive oxygen species (ROS) are formed in photosystem II (PSII) under various types of abiotic and biotic stresses. It is considered that ROS play a role in chloroplast-to-nucleus retrograde signaling, which changes the nuclear gene expression. However, ...

    Abstract Reactive oxygen species (ROS) are formed in photosystem II (PSII) under various types of abiotic and biotic stresses. It is considered that ROS play a role in chloroplast-to-nucleus retrograde signaling, which changes the nuclear gene expression. However, as ROS lifetime and diffusion are restricted due to the high reactivity towards biomolecules (lipids, pigments, and proteins) and the spatial specificity of signal transduction is low, it is not entirely clear how ROS might transduce signal from the chloroplasts to the nucleus. Biomolecule oxidation was formerly connected solely with damage; nevertheless, the evidence appears that oxidatively modified lipids and pigments are be involved in chloroplast-to-nucleus retrograde signaling due to their long diffusion distance. Moreover, oxidatively modified proteins show high spatial specificity; however, their role in signal transduction from chloroplasts to the nucleus has not been proven yet. The review attempts to summarize and evaluate the evidence for the involvement of ROS in oxidative signaling in PSII.
    Schlagwörter biochemical compounds ; chloroplasts ; gene expression ; oxidation ; photosystem II ; reactive oxygen species ; signal transduction
    Sprache Englisch
    Erscheinungsverlauf 2022-06
    Umfang p. 245-260.
    Erscheinungsort Springer Netherlands
    Dokumenttyp Artikel ; Online
    Anmerkung Review
    ZDB-ID 1475688-2
    ISSN 1573-5079 ; 0166-8595
    ISSN (online) 1573-5079
    ISSN 0166-8595
    DOI 10.1007/s11120-022-00922-x
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  5. Artikel ; Online: Detection and characterization of free oxygen radicals induced protein adduct formation in differentiating macrophages.

    Manoharan, Renuka Ramalingam / Sedlářová, Michaela / Pospíšil, Pavel / Prasad, Ankush

    Biochimica et biophysica acta. General subjects

    2023  Band 1867, Heft 5, Seite(n) 130324

    Abstract: Reactive oxygen species play a key role in cellular homeostasis and redox signaling at physiological levels, where excessive production affects the function and integrity of macromolecules, specifically proteins. Therefore, it is important to define ... ...

    Abstract Reactive oxygen species play a key role in cellular homeostasis and redox signaling at physiological levels, where excessive production affects the function and integrity of macromolecules, specifically proteins. Therefore, it is important to define radical-mediated proteotoxic stress in macrophages and identify target protein to prevent tissue dysfunction. A well employed, THP-1 cell line was utilized as in vitro model to study immune response and herein we employ immuno-spin trapping technique to investigate radical-mediated protein oxidation in macrophages. Hydroxyl radical formation along macrophage differentiation was confirmed by electron paramagnetic resonance along with confocal laser scanning microscopy using hydroxyphenyl fluorescein. Lipid peroxidation product, malondialdehyde, generated under experimental conditions as detected using swallow-tailed perylene derivative fluorescence observed by confocal laser scanning microscopy and high-performance liquid chromatography, respectively. The results obtained from this study warrant further corroboration and study of specific proteins involved in the macrophage activation and their role in inflammations.
    Mesh-Begriff(e) Reactive Oxygen Species/metabolism ; Free Radicals/analysis ; Free Radicals/metabolism ; Spin Trapping/methods ; Electron Spin Resonance Spectroscopy/methods ; Macrophages/metabolism ; Proteins/chemistry
    Chemische Substanzen Reactive Oxygen Species ; Free Radicals ; Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-02-10
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 60-7
    ISSN 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650 ; 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    ISSN (online) 1872-8006 ; 1879-2596 ; 1879-260X ; 1879-2642 ; 1879-2618 ; 1879-2650
    ISSN 0006-3002 ; 0005-2728 ; 0005-2736 ; 0304-4165 ; 0167-4838 ; 1388-1981 ; 0167-4889 ; 0167-4781 ; 0304-419X ; 1570-9639 ; 0925-4439 ; 1874-9399
    DOI 10.1016/j.bbagen.2023.130324
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Scanning Electrochemical Microscopy-Somatic Cell Count as a Method for Diagnosis of Bovine Mastitis.

    Kasai, Shigenobu / Prasad, Ankush / Kumagai, Ryoma / Takanohashi, Keita

    Biology

    2022  Band 11, Heft 4

    Abstract: The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × ... ...

    Abstract The method to diagnose mastitis is generally the somatic cell count (SCC) by flow cytometry measurement. When the number of somatic cells in raw milk is 2.0 × 10
    Sprache Englisch
    Erscheinungsdatum 2022-04-01
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2661517-4
    ISSN 2079-7737
    ISSN 2079-7737
    DOI 10.3390/biology11040549
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  7. Artikel ; Online: Reactive oxygen species in photosystem II: relevance for oxidative signaling.

    Pospíšil, Pavel / Kumar, Aditya / Prasad, Ankush

    Photosynthesis research

    2022  Band 152, Heft 3, Seite(n) 245–260

    Abstract: Reactive oxygen species (ROS) are formed in photosystem II (PSII) under various types of abiotic and biotic stresses. It is considered that ROS play a role in chloroplast-to-nucleus retrograde signaling, which changes the nuclear gene expression. However, ...

    Abstract Reactive oxygen species (ROS) are formed in photosystem II (PSII) under various types of abiotic and biotic stresses. It is considered that ROS play a role in chloroplast-to-nucleus retrograde signaling, which changes the nuclear gene expression. However, as ROS lifetime and diffusion are restricted due to the high reactivity towards biomolecules (lipids, pigments, and proteins) and the spatial specificity of signal transduction is low, it is not entirely clear how ROS might transduce signal from the chloroplasts to the nucleus. Biomolecule oxidation was formerly connected solely with damage; nevertheless, the evidence appears that oxidatively modified lipids and pigments are be involved in chloroplast-to-nucleus retrograde signaling due to their long diffusion distance. Moreover, oxidatively modified proteins show high spatial specificity; however, their role in signal transduction from chloroplasts to the nucleus has not been proven yet. The review attempts to summarize and evaluate the evidence for the involvement of ROS in oxidative signaling in PSII.
    Mesh-Begriff(e) Chloroplasts/metabolism ; Lipids ; Oxidative Stress ; Photosystem II Protein Complex/metabolism ; Reactive Oxygen Species/metabolism ; Signal Transduction/physiology
    Chemische Substanzen Lipids ; Photosystem II Protein Complex ; Reactive Oxygen Species
    Sprache Englisch
    Erscheinungsdatum 2022-05-28
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Review
    ZDB-ID 1475688-2
    ISSN 1573-5079 ; 0166-8595
    ISSN (online) 1573-5079
    ISSN 0166-8595
    DOI 10.1007/s11120-022-00922-x
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  8. Artikel ; Online: Detection and characterization of free radicals induced protein adducts formation in differentiating macrophages

    Manoharan, Renuka Ramalingam / Sedlářová, Michaela / Pospisil, Pavel / Prasad, Ankush

    BBA - General Subjects. 2023, p.130324-

    2023  , Seite(n) 130324–

    Abstract: Reactive oxygen species play a key role in cellular homeostasis and redox signaling at physiological levels, where excessive production affects the function and integrity of macromolecules, specifically proteins. Therefore, it is important to define free ...

    Abstract Reactive oxygen species play a key role in cellular homeostasis and redox signaling at physiological levels, where excessive production affects the function and integrity of macromolecules, specifically proteins. Therefore, it is important to define free radical-mediated proteotoxic stress in macrophages and identify target protein to prevent tissue dysfunction. A well employed, THP-1 cell line was utilized as in vitro model to study immune response and herein we employ immuno-spin trapping technique to investigate free radical-mediated protein oxidation in macrophages. Hydroxyl radical formation along macrophage differentiation was confirmed by electron paramagnetic resonance along with confocal laser scanning microscopy using hydroxyphenyl fluorescein. Lipid peroxidation product, malondialdehyde, generated under experimental conditions as detected using swallow-tailed perylene derivative fluorescence observed by confocal laser scanning microscopy and high-performance liquid chromatography, respectively. The results obtained from this study warrant further corroboration and study of specific proteins involved in the macrophage activation and their role in inflammations.
    Schlagwörter cell lines ; electron paramagnetic resonance spectroscopy ; fluorescein ; fluorescence ; high performance liquid chromatography ; homeostasis ; hydroxyl radicals ; lipid peroxidation ; macrophage activation ; macrophages ; malondialdehyde ; microscopy ; models ; oxidation ; Reactive oxygen species ; Macrophage ; Phorbol 12-myristate 13-acetate ; All-trans retinoic acid ; Lipopolysaccharide ; Protein oxidation ; ROS ; EPR spectroscopy ; CLSM ; PMA ; RIPA ; ATRA ; LPS ; DNPH ; DIC
    Sprache Englisch
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel ; Online
    Anmerkung Pre-press version
    ZDB-ID 840755-1
    ISSN 0304-4165
    ISSN 0304-4165
    DOI 10.1016/j.bbagen.2023.130324
    Datenquelle NAL Katalog (AGRICOLA)

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  9. Artikel ; Online: Imaging and Characterization of Oxidative Protein Modifications in Skin.

    Prasad, Ankush / Duchová, Hana / Manoharan, Renuka Ramalingam / Rathi, Deepak / Pospíšil, Pavel

    International journal of molecular sciences

    2023  Band 24, Heft 4

    Abstract: Skin plays an important role in protection, metabolism, thermoregulation, sensation, and excretion whilst being consistently exposed to environmental aggression, including biotic and abiotic stresses. During the generation of oxidative stress in the skin, ...

    Abstract Skin plays an important role in protection, metabolism, thermoregulation, sensation, and excretion whilst being consistently exposed to environmental aggression, including biotic and abiotic stresses. During the generation of oxidative stress in the skin, the epidermal and dermal cells are generally regarded as the most affected regions. The participation of reactive oxygen species (ROS) as a result of environmental fluctuations has been experimentally proven by several researchers and is well known to contribute to ultra-weak photon emission via the oxidation of biomolecules (lipids, proteins, and nucleic acids). More recently, ultra-weak photon emission detection techniques have been introduced to investigate the conditions of oxidative stress in various living systems in in vivo, ex vivo and in vitro studies. Research into two-dimensional photon imaging is drawing growing attention because of its application as a non-invasive tool. We monitored spontaneous and stress-induced ultra-weak photon emission under the exogenous application of a Fenton reagent. The results showed a marked difference in the ultra-weak photon emission. Overall, these results suggest that triplet carbonyl (
    Mesh-Begriff(e) Reactive Oxygen Species/metabolism ; Hydrogen Peroxide/metabolism ; Skin/metabolism ; Oxidative Stress ; Oxidation-Reduction ; Proteins/metabolism
    Chemische Substanzen Reactive Oxygen Species ; Hydrogen Peroxide (BBX060AN9V) ; Proteins
    Sprache Englisch
    Erscheinungsdatum 2023-02-16
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms24043981
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  10. Artikel ; Online: Differential effects of ascorbic acid on monocytic cell morphology and protein modification: Shifting from pro-oxidative to antioxidant properties.

    Prasad, Ankush / Rathi, Deepak / Sedlářová, Michaela / Manoharan, Renuka Ramalingam / Průdková, Eliška / Pospíšil, Pavel

    Biochemistry and biophysics reports

    2023  Band 37, Seite(n) 101622

    Abstract: In this study, we investigated the properties of ascorbic acid (vitamin C), which is a naturally occurring water-soluble vitamin. Our goal is to evaluate its pro-oxidative and/or antioxidant capabilities. To do this, we initially used a confocal laser ... ...

    Abstract In this study, we investigated the properties of ascorbic acid (vitamin C), which is a naturally occurring water-soluble vitamin. Our goal is to evaluate its pro-oxidative and/or antioxidant capabilities. To do this, we initially used a confocal laser scanning microscope (CLSM) to visualize the differentiation pattern in U-937 cells under the treatment of variable concentrations of ascorbic acid. Prior to induction, U-937 cells showed a spherical morphology. After treatment, significant morphological changes were observed in the form of prominent pseudopodia and amoeboid structures. Interestingly, pseudopodia incidences increased with an increase in ascorbic acid concentrations. In addition, our analysis of protein modification using anti-malondialdehyde antibodies showed changes in more than one protein. The findings reveal the link between the differentiation of U-937 cells into macrophages and the protein modifications triggered by the production of reactive oxygen species when U-937 cells are exposed to ascorbic acid. Furthermore, the transformation of ascorbic acid from a pro-oxidative to an antioxidant property is also demonstrated.
    Sprache Englisch
    Erscheinungsdatum 2023-12-27
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 2831046-9
    ISSN 2405-5808 ; 2405-5808
    ISSN (online) 2405-5808
    ISSN 2405-5808
    DOI 10.1016/j.bbrep.2023.101622
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