Artikel ; Online: Identification of catalytically distinct arylalkylamine N-acetyltransferase splicoforms from Tribolium castaneum.
Protein expression and purification
2020 Band 175, Seite(n) 105695
Abstract: The assumption that structural or sequential homology between enzymes implies functional homology is a common misconception. Through in-depth structural and kinetic analysis, we are now beginning to understand the minute differences in primary structure ... ...
Abstract | The assumption that structural or sequential homology between enzymes implies functional homology is a common misconception. Through in-depth structural and kinetic analysis, we are now beginning to understand the minute differences in primary structure that can alter the function of an enzyme completely. Alternative splicing is one method for which the activity of an enzyme can be controlled, simply by altering its length. Arylalkylamine N-acetyltransferase A (AANATA) in D. melanogaster, which catalyzes the N-acetylation of biogenic amines, has multiple splicoforms - alternatively spliced enzyme isoforms - with differing tissue distribution. As demonstrated here, AANAT1 from Tribolium castaneum is another such enzyme with multiple splicoforms. A screening assay was developed and utilized to determine that, despite only a 35 amino acid truncation, the shortened form of TcAANAT1 is a more active form of the enzyme. This implies regulation of enzyme metabolic activity via alternative splicing. |
---|---|
Mesh-Begriff(e) | Alternative Splicing ; Animals ; Arylalkylamine N-Acetyltransferase/biosynthesis ; Arylalkylamine N-Acetyltransferase/genetics ; Drosophila melanogaster ; Insect Proteins/biosynthesis ; Insect Proteins/genetics ; Isoenzymes/biosynthesis ; Isoenzymes/genetics ; Tribolium/enzymology ; Tribolium/genetics |
Chemische Substanzen | Insect Proteins ; Isoenzymes ; Arylalkylamine N-Acetyltransferase (EC 2.3.1.87) |
Sprache | Englisch |
Erscheinungsdatum | 2020-07-16 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
ZDB-ID | 1055455-5 |
ISSN | 1096-0279 ; 1046-5928 |
ISSN (online) | 1096-0279 |
ISSN | 1046-5928 |
DOI | 10.1016/j.pep.2020.105695 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Volltext online
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
Zs.A 3084: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.