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  1. Artikel ; Online: Egg Case Protein 3: A Constituent of Black Widow Spider Tubuliform Silk.

    Shanafelt, Mikayla / Larracas, Camille / Dyrness, Simmone / Hekman, Ryan / La Mattina-Hawkins, Coby / Rabara, Taylor / Wu, Wilson / Vierra, Craig A

    Molecules (Basel, Switzerland)

    2021  Band 26, Heft 16

    Abstract: Spider silk has outstanding mechanical properties, rivaling some of the best materials on the planet. Biochemical analyses of tubuliform silk have led to the identification of TuSp1, egg case protein 1, and egg case protein 2. TuSp1 belongs to the ... ...

    Abstract Spider silk has outstanding mechanical properties, rivaling some of the best materials on the planet. Biochemical analyses of tubuliform silk have led to the identification of TuSp1, egg case protein 1, and egg case protein 2. TuSp1 belongs to the spidroin superfamily, containing a non-repetitive
    Mesh-Begriff(e) Amino Acid Sequence ; Animal Structures/metabolism ; Animals ; Black Widow Spider/chemistry ; Egg Proteins/chemistry ; Egg Proteins/genetics ; Egg Proteins/metabolism ; Female ; Fibroins/chemistry ; Gene Expression Regulation ; Ovum/metabolism ; Ovum/ultrastructure ; Proteomics ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Tandem Mass Spectrometry
    Chemische Substanzen Egg Proteins ; RNA, Messenger ; Fibroins (9007-76-5)
    Sprache Englisch
    Erscheinungsdatum 2021-08-22
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules26165088
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.MO 81: Hefte anzeigen

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  2. Artikel ; Online: Non-Native Amino Acid Click Chemistry-Based Technology for Site-Specific Polysaccharide Conjugation to a Bacterial Protein Serving as Both Carrier and Vaccine Antigen.

    Kapoor, Neeraj / Uchiyama, Satoshi / Pill, Lucy / Bautista, Leslie / Sedra, Angie / Yin, Lu / Regan, Maritoni / Chu, Ellen / Rabara, Taylor / Wong, Melissa / Davey, Peter / Fairman, Jeff / Nizet, Victor

    ACS omega

    2022  Band 7, Heft 28, Seite(n) 24111–24120

    Abstract: Surface-expressed bacterial polysaccharides are important vaccine antigens but must be conjugated to a carrier protein for efficient antigen presentation and development of strong memory B cell and antibody responses, especially in young children. The ... ...

    Abstract Surface-expressed bacterial polysaccharides are important vaccine antigens but must be conjugated to a carrier protein for efficient antigen presentation and development of strong memory B cell and antibody responses, especially in young children. The commonly used protein carriers include tetanus toxoid (TT), diphtheria toxoid (DT), and its derivative CRM197, but carrier-induced epitopic suppression and bystander interference may limit the expanded use of the same carriers in the pediatric immunization schedule. Recent efforts to develop a vaccine against the major human pathogen group A
    Sprache Englisch
    Erscheinungsdatum 2022-07-11
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2470-1343
    ISSN (online) 2470-1343
    DOI 10.1021/acsomega.1c07360
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Structural Characterization of Black Widow Spider Dragline Silk Proteins CRP1 and CRP4.

    Shanafelt, Mikayla / Rabara, Taylor / MacArt, Danielle / Williams, Caroline / Hekman, Ryan / Joo, Hyun / Tsai, Jerry / Vierra, Craig

    Molecules (Basel, Switzerland)

    2020  Band 25, Heft 14

    Abstract: Spider dragline silk represents a biomaterial with outstanding mechanical properties, possessing high-tensile strength and toughness. In black widows at least eight different proteins have been identified as constituents of dragline silk. These represent ...

    Abstract Spider dragline silk represents a biomaterial with outstanding mechanical properties, possessing high-tensile strength and toughness. In black widows at least eight different proteins have been identified as constituents of dragline silk. These represent major ampullate spidroins MaSp1, MaSp2, MaSp', and several low-molecular weight cysteine-rich protein (CRP) family members, including CRP1, CRP2, and CRP4. Molecular modeling predicts that CRPs contain a cystine slipknot motif, but experimental evidence to support this assertion remains to be reported. To advance scientific knowledge regarding CRP function, we recombinantly expressed and purified CRP1 and CRP4 from bacteria and investigated their secondary structure using circular dichroism (CD) under different chemical and physical conditions. We demonstrate by far-UV CD spectroscopy that these proteins contain similar secondary structure, having substantial amounts of random coil conformation, followed by lower levels of beta sheet, alpha helical and beta turn structures. CRPs are thermally and pH stable; however, treatment with reagents that disrupt disulfide bonds impact their structural conformations. Cross-linking mass spectrometry (XL-MS) data also support computational models of CRP1. Taken together, the chemical and thermal stability of CRPs, the cross-linking data, coupled with the structural sensitivity to reducing agents, are experimentally consistent with the supposition CRPs are cystine slipknot proteins.
    Mesh-Begriff(e) Amino Acid Sequence ; Animals ; Black Widow Spider/chemistry ; Hydrogen-Ion Concentration ; Insect Proteins/chemistry ; Insect Proteins/isolation & purification ; Models, Molecular ; Protein Conformation ; Protein Folding ; Protein Structure, Secondary ; Recombinant Proteins ; Silk/chemistry ; Tandem Mass Spectrometry
    Chemische Substanzen Insect Proteins ; Recombinant Proteins ; Silk
    Sprache Englisch
    Erscheinungsdatum 2020-07-14
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 1413402-0
    ISSN 1420-3049 ; 1431-5165 ; 1420-3049
    ISSN (online) 1420-3049
    ISSN 1431-5165 ; 1420-3049
    DOI 10.3390/molecules25143212
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: SARS-CoV-2 B.1.1.7 (alpha) and B.1.351 (beta) variants induce pathogenic patterns in K18-hACE2 transgenic mice distinct from early strains.

    Radvak, Peter / Kwon, Hyung-Joon / Kosikova, Martina / Ortega-Rodriguez, Uriel / Xiang, Ruoxuan / Phue, Je-Nie / Shen, Rong-Fong / Rozzelle, James / Kapoor, Neeraj / Rabara, Taylor / Fairman, Jeff / Xie, Hang

    Nature communications

    2021  Band 12, Heft 1, Seite(n) 6559

    Abstract: SARS-CoV-2 variants of concern (VOC) B.1.1.7 (alpha) and B.1.351 (beta) show increased transmissibility and enhanced antibody neutralization resistance. Here we demonstrate in K18-hACE2 transgenic mice that B.1.1.7 and B.1.351 are 100-fold more lethal ... ...

    Abstract SARS-CoV-2 variants of concern (VOC) B.1.1.7 (alpha) and B.1.351 (beta) show increased transmissibility and enhanced antibody neutralization resistance. Here we demonstrate in K18-hACE2 transgenic mice that B.1.1.7 and B.1.351 are 100-fold more lethal than the original SARS-CoV-2 bearing 614D. B.1.1.7 and B.1.351 cause more severe organ lesions in K18-hACE2 mice than early SARS-CoV-2 strains bearing 614D or 614G, with B.1.1.7 and B.1.351 infection resulting in distinct tissue-specific cytokine signatures, significant D-dimer depositions in vital organs and less pulmonary hypoxia signaling before death. However, K18-hACE2 mice with prior infection of early SARS-CoV-2 strains or intramuscular immunization of viral spike or receptor binding domain are resistant to the lethal reinfection of B.1.1.7 or B.1.351, despite having reduced neutralization titers against these VOC than early strains. Our results thus distinguish pathogenic patterns in K18-hACE2 mice caused by B.1.1.7 and B.1.351 infection from those induced by early SARS-CoV-2 strains, and help inform potential medical interventions for combating COVID-19.
    Mesh-Begriff(e) Angiotensin-Converting Enzyme 2/immunology ; Angiotensin-Converting Enzyme 2/metabolism ; Animals ; Antibodies, Neutralizing/immunology ; COVID-19/genetics ; COVID-19/pathology ; COVID-19/virology ; Cell Line ; Chlorocebus aethiops ; Cytokines/immunology ; Disease Models, Animal ; Female ; Fibrin Fibrinogen Degradation Products/immunology ; Hypoxia/virology ; Lung/metabolism ; Lung/pathology ; Lung/virology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; SARS-CoV-2/classification ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/pathogenicity ; Spike Glycoprotein, Coronavirus/immunology
    Chemische Substanzen Antibodies, Neutralizing ; Cytokines ; Fibrin Fibrinogen Degradation Products ; Spike Glycoprotein, Coronavirus ; fibrin fragment D ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
    Sprache Englisch
    Erscheinungsdatum 2021-11-12
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-021-26803-w
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Role of

    Naranjo, Christopher A / Jivan, Anita D / Vo, Maria N / de Sa Campos, Katherine H / Deyarmin, Jared S / Hekman, Ryan M / Uribe, Christina / Hang, Aaron / Her, Kai / Fong, Michelle M / Choi, Joyce J / Chou, Caroline / Rabara, Taylor R / Myers, Gina / Moua, Pachai / Thor, Der / Risser, Douglas D / Vierra, Craig A / Franz, Andreas H /
    Lin-Cereghino, Joan / Lin-Cereghino, Geoff P

    Applied and environmental microbiology

    2019  Band 85, Heft 24

    Abstract: The methylotrophic ... ...

    Abstract The methylotrophic yeast
    Mesh-Begriff(e) Amino Acid Sequence ; Bacterial Secretion Systems ; Cell Wall/chemistry ; Cloning, Molecular ; Endoplasmic Reticulum/metabolism ; Fungal Proteins/genetics ; Fungal Proteins/metabolism ; Gene Expression Regulation, Fungal ; Molecular Chaperones/metabolism ; Pichia/genetics ; Pichia/metabolism ; Protein Kinase C/metabolism ; Protein Translocation Systems/genetics ; Protein Translocation Systems/metabolism ; Proteomics ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism ; Saccharomyces cerevisiae Proteins/metabolism
    Chemische Substanzen Bacterial Secretion Systems ; Fungal Proteins ; Molecular Chaperones ; Protein Translocation Systems ; Recombinant Proteins ; Saccharomyces cerevisiae Proteins ; PKC1 protein, S cerevisiae (EC 2.7.11.13) ; Protein Kinase C (EC 2.7.11.13)
    Sprache Englisch
    Erscheinungsdatum 2019-11-27
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 223011-2
    ISSN 1098-5336 ; 0099-2240
    ISSN (online) 1098-5336
    ISSN 0099-2240
    DOI 10.1128/AEM.01615-19
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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