Artikel ; Online: Chemical shift assignments of the biotin carboxyl carrier protein domain of L. major Methylcrotonyl-CoA carboxylase.
2021 Band 15, Heft 2, Seite(n) 249–253
Abstract: Methylcrotonyl-CoA carboxylase (MCCC) is a biotin dependent enzyme, that plays a crucial role in leucine metabolism. The enzyme comprises a biotin carboxylase (BC), a carboxyltransferase (CT), and a biotin carboxyl carrier protein (BCCP) domain. MCCC is ... ...
Abstract | Methylcrotonyl-CoA carboxylase (MCCC) is a biotin dependent enzyme, that plays a crucial role in leucine metabolism. The enzyme comprises a biotin carboxylase (BC), a carboxyltransferase (CT), and a biotin carboxyl carrier protein (BCCP) domain. MCCC is synthesized as an apo-protein, and is posttranslationally modified at a lysine residue, conserved in the biotin carboxyl carrier protein (BCCP) domain. In order to understand the structure, function and interactions of L. major MCCC, we have expressed and characterized its domains. Here we report the complete chemical shift assignments of MCCC BCCP domain of L. major. Furthermore, we have used the assignments to generate a model of the same, using CS-Rosetta. We have also followed its chemical shift perturbations upon biotin modification. Changes were observed at the lysine 51 amide, that undergoes biotin modification, and a few others present in its immediate neighborhood. |
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Mesh-Begriff(e) | Nuclear Magnetic Resonance, Biomolecular |
Sprache | Englisch |
Erscheinungsdatum | 2021-03-22 |
Erscheinungsland | Netherlands |
Dokumenttyp | Journal Article |
ZDB-ID | 2388861-1 |
ISSN | 1874-270X ; 1874-2718 |
ISSN (online) | 1874-270X |
ISSN | 1874-2718 |
DOI | 10.1007/s12104-021-10013-y |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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