LIVIVO - Das Suchportal für Lebenswissenschaften

switch to English language
Zurück zur letzten Suche Suchhistorie
Erweiterte Suche

Ihre letzten Suchen

  1. AU="Rolland, Simon"
  2. AU="Fitz Gerald, John D"

Suchergebnis

Treffer 1 - 10 von insgesamt 10

Suchoptionen

  1. Artikel ; Online: Frequency and factors associated with infusion-related local complications of vancomycin on peripheral venous catheters.

    Ammar, Helmi / Rolland, Simon / Jouffroy, Romain / Dubert, Marie / Le Beller, Christine / Podglajen, Isabelle / Lillo-Lelouet, Agnès / Lebeaux, David / Bensaid, Samuel

    The Journal of antimicrobial chemotherapy

    2023  Band 78, Heft 4, Seite(n) 1050–1054

    Abstract: Background: Vancomycin is a reference antibiotic against methicillin-resistant staphylococci. Its administration is associated with infusion-related local complications (IRLC). To reduce this risk, it has been proposed to increase vancomycin dilution in ...

    Abstract Background: Vancomycin is a reference antibiotic against methicillin-resistant staphylococci. Its administration is associated with infusion-related local complications (IRLC). To reduce this risk, it has been proposed to increase vancomycin dilution in the IV bag and to perform continuous infusion using the volumetric pump. The aim of our study was to assess the safety of peripheral infusion of vancomycin with the volumetric pump.
    Objectives: To compare the frequency of IRLC between patients receiving vancomycin and those receiving β-lactam (BL) antibiotics. Our secondary objective was to assess factors associated with the occurrence of IRLC.
    Patients and methods: We conducted a prospective observational study in a French tertiary hospital. Between February 2021 and November 2021, we included all patients receiving continuous infusions of vancomycin or BL through a peripherally inserted venous catheter (PIVC). The primary endpoint was the occurrence of IRLC on Day 1 (D1).
    Results: We included 168 patients (56 vancomycin, 112 BL). At D1, 14 patients (25%) presented IRLC in the vancomycin group versus 11 patients (10%) in the BL group (P = 0.01). There was significantly more IRLC in the group receiving vancomycin at an infused concentration above 5 mg/mL than those receiving BL (8/15, 53.3% versus 11/112, 10%, respectively, P < 0.01). However, no significant difference was observed between patients receiving infused vancomycin concentration ≤5 mg/mL and patients receiving BL (P = 0.4).
    Conclusion: Our data support safe administration of vancomycin if infused at a concentration under 5 mg/mL, through the volumetric pump on PIVC.
    Mesh-Begriff(e) Humans ; Vancomycin/adverse effects ; Anti-Bacterial Agents/therapeutic use ; Infusions, Intravenous ; Staphylococcus ; Catheters
    Chemische Substanzen Vancomycin (6Q205EH1VU) ; Anti-Bacterial Agents
    Sprache Englisch
    Erscheinungsdatum 2023-03-07
    Erscheinungsland England
    Dokumenttyp Observational Study ; Journal Article
    ZDB-ID 191709-2
    ISSN 1460-2091 ; 0305-7453
    ISSN (online) 1460-2091
    ISSN 0305-7453
    DOI 10.1093/jac/dkad044
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1197: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 124: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  2. Artikel: Switched on to mini grids

    Rolland, Simon

    Renewable energy focus. 2011 , v. 12, no. 3

    2011  

    Abstract: A BILLION AND a half people around the world still don't have access to electricity. Could hybrid mini grids be the best way to provide electricity – particularly to rural, often isolated, areas? ...

    Abstract A BILLION AND a half people around the world still don't have access to electricity. Could hybrid mini grids be the best way to provide electricity – particularly to rural, often isolated, areas?
    Schlagwörter electricity ; hybrids ; people
    Sprache Englisch
    Erscheinungsverlauf 2011-05
    Umfang p. 10-12.
    Dokumenttyp Artikel
    ZDB-ID 2451929-7
    ISSN 1878-0229 ; 1755-0084
    ISSN (online) 1878-0229
    ISSN 1755-0084
    DOI 10.1016/S1755-0084(11)70054-0
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  3. Artikel ; Online: TIVAP-related infection due to Gram-negative aerobic bacilli: should TIVAP stay or should it go?

    Rolland, Simon / Kassis-Chikhani, Najiby / Auclin, Edouard / Bensaid, Samuel / Bidaud, Anne-Laure / Gerlinger, Marie-Paule / Blez, Damien / Mainardi, Jean-Luc / Lebeaux, David / Dubert, Marie

    European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

    2022  

    Abstract: We aimed to describe the outcome of totally implantable venous-access port (TIVAP)-related infections due to Gram-negative aerobic bacilli (Pseudomonas aeruginosa and other Pseudomonas spp., Acinetobacter spp., and Stenotrophomonas maltophilia), or GNAB, ...

    Abstract We aimed to describe the outcome of totally implantable venous-access port (TIVAP)-related infections due to Gram-negative aerobic bacilli (Pseudomonas aeruginosa and other Pseudomonas spp., Acinetobacter spp., and Stenotrophomonas maltophilia), or GNAB, and assess the safety of conservative treatment. We conducted a retrospective study in a French teaching hospital, from January 2016 to December 2020, including adult patients treated for TIVAP-related infection due to GNAB. Success of conservative treatment was defined as a functional TIVAP 3 months after infection with no recurrence. We performed a bivariate analysis and analyzed causes for treatment failure. We included 68 patients (53 TIVAP-related bloodstream infections, 11 TIVAP-related infections, and 4 probable TIVAP-related infections) due to GNAB, mostly P. aeruginosa (50/68, 74%). TIVAP removal was initially decided for 49/68 patients (72%). Among the 19/68 (28%) patients with conservative treatment (all for infections caused by P. aeruginosa), 5/19 (26%) had successful treatment, 7/19 (37%) experienced failure (without sepsis or septic shock), 6/19 (32%) died within 3 months without TIVAP removal and no signs of infection recurrence, and 1 patient had TIVAP removal as it was no longer required. TIVAP-related infections caused by GNAB frequently require TIVAP removal. Conservative treatment can be performed in selected patients with a non-complicated infection caused by P. aeruginosa, who can benefit from the continuation of antineoplastic chemotherapy or palliative care. Treatment failures were not associated with sepsis or septic shock.
    Sprache Englisch
    Erscheinungsdatum 2022-12-06
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 603155-9
    ISSN 1435-4373 ; 0934-9723 ; 0722-2211
    ISSN (online) 1435-4373
    ISSN 0934-9723 ; 0722-2211
    DOI 10.1007/s10096-022-04537-x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 1732: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  4. Artikel ; Online: Impact of the COVID-19 pandemic on the homeless: results from a retrospective closed cohort in France (March-May 2020).

    Husain, Maya / Rachline, Anne / Cousien, Anthony / Rolland, Simon / Rouzaud, Claire / Ferre, Valentine Marie / Gomez, Maria Vargas / Le Teurnier, Maï / Wicky-Thisse, Marie / Descamps, Diane / Yazdanpanah, Yazdan / Charpentier, Charlotte / Pasquet-Cadre, Armelle

    Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases

    2021  Band 27, Heft 10, Seite(n) 1520.e1–1520.e5

    Abstract: Objectives: To evaluate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, hospitalization and fatality rates in residents of homeless shelters run by Samusocial of Paris.: Methods: We conducted a retrospective serological study ... ...

    Abstract Objectives: To evaluate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, hospitalization and fatality rates in residents of homeless shelters run by Samusocial of Paris.
    Methods: We conducted a retrospective serological study between July and August 2020 on all residents and staff members of three homeless shelters run by Samusocial of Paris: two centres providing healthcare accommodation (HCA) and one a women's dormitory. We included all adults present in the shelters or who died of a proven SARS-CoV-2 infection during the first wave (March-May). SARS-CoV-2 antibodies were detected in serum samples using the SARS-CoV-2 IgG Architect (Abbott) test. Any participant with a positive PCR or serology was defined as a confirmed SARS-CoV-2 case.
    Results: We included 100 residents and 83 staff members. The confirmed SARS-CoV-2 rate by PCR or serology was 72/100 (72.0%) for residents and 17/83 (20.5%) for staff members. Women accommodated in the dormitory had the highest infection rate (90.6%). The hospitalization rate in residents was 17/72 (23.6%) and the death rate 4/72 (5.6%). All hospitalizations and deaths occurred among HCA residents. Among the residents of HCA shelters, 34/68 (50%) presented at least two comorbidity factors associated with being at high risk for severe SARS-CoV-2 infection.
    Conclusion: The SARS-CoV-2 infection rate was high in residents of these homeless shelters (10.6% seroprevalence in the Île-de-France region during the first wave). Severe SARS-CoV-2 infection was highly associated with the prevalence of comorbidities. This population should be considered as a priority in vaccination campaigns and in access to individual housing units when at risk.
    Mesh-Begriff(e) Adult ; COVID-19/blood ; COVID-19/epidemiology ; COVID-19/mortality ; Female ; France/epidemiology ; Homeless Persons/statistics & numerical data ; Hospitalization ; Humans ; Incidence ; Male ; Middle Aged ; Prevalence ; Retrospective Studies ; Risk Factors ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Seroepidemiologic Studies
    Sprache Englisch
    Erscheinungsdatum 2021-06-07
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 1328418-6
    ISSN 1469-0691 ; 1470-9465 ; 1198-743X
    ISSN (online) 1469-0691
    ISSN 1470-9465 ; 1198-743X
    DOI 10.1016/j.cmi.2021.05.039
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 4541: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)
    Zs.MO 284: Hefte anzeigen

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  5. Artikel ; Online: A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations.

    Grzelak, Ludivine / Temmam, Sarah / Planchais, Cyril / Demeret, Caroline / Tondeur, Laura / Huon, Christèle / Guivel-Benhassine, Florence / Staropoli, Isabelle / Chazal, Maxime / Dufloo, Jeremy / Planas, Delphine / Buchrieser, Julian / Rajah, Maaran Michael / Robinot, Remy / Porrot, Françoise / Albert, Mélanie / Chen, Kuang-Yu / Crescenzo-Chaigne, Bernadette / Donati, Flora /
    Anna, François / Souque, Philippe / Gransagne, Marion / Bellalou, Jacques / Nowakowski, Mireille / Backovic, Marija / Bouadma, Lila / Le Fevre, Lucie / Le Hingrat, Quentin / Descamps, Diane / Pourbaix, Annabelle / Laouénan, Cédric / Ghosn, Jade / Yazdanpanah, Yazdan / Besombes, Camille / Jolly, Nathalie / Pellerin-Fernandes, Sandrine / Cheny, Olivia / Ungeheuer, Marie-Noëlle / Mellon, Guillaume / Morel, Pascal / Rolland, Simon / Rey, Felix A / Behillil, Sylvie / Enouf, Vincent / Lemaitre, Audrey / Créach, Marie-Aude / Petres, Stephane / Escriou, Nicolas / Charneau, Pierre / Fontanet, Arnaud / Hoen, Bruno / Bruel, Timothée / Eloit, Marc / Mouquet, Hugo / Schwartz, Olivier / van der Werf, Sylvie

    Science translational medicine

    2020  Band 12, Heft 559

    Abstract: It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts ... ...

    Abstract It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts of anti-SARS-CoV-2 antibodies in serum samples obtained from 491 healthy individuals before the SARS-CoV-2 pandemic, 51 individuals hospitalized with COVID-19, 209 suspected cases of COVID-19 with mild symptoms, and 200 healthy blood donors. We used two ELISA assays that recognized the full-length nucleoprotein (N) or trimeric spike (S) protein ectodomain of SARS-CoV-2. In addition, we developed the S-Flow assay that recognized the S protein expressed at the cell surface using flow cytometry, and the luciferase immunoprecipitation system (LIPS) assay that recognized diverse SARS-CoV-2 antigens including the S1 domain and the carboxyl-terminal domain of N by immunoprecipitation. We obtained similar results with the four serological assays. Differences in sensitivity were attributed to the technique and the antigen used. High anti-SARS-CoV-2 antibody titers were associated with neutralization activity, which was assessed using infectious SARS-CoV-2 or lentiviral-S pseudotype virus. In hospitalized patients with COVID-19, seroconversion and virus neutralization occurred between 5 and 14 days after symptom onset, confirming previous studies. Seropositivity was detected in 32% of mildly symptomatic individuals within 15 days of symptom onset and in 3% of healthy blood donors. The four antibody assays that we used enabled a broad evaluation of SARS-CoV-2 seroprevalence and antibody profiling in different subpopulations within one region.
    Mesh-Begriff(e) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Antibodies, Viral/blood ; Betacoronavirus/immunology ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Cohort Studies ; Coronavirus Infections/diagnosis ; Coronavirus Infections/epidemiology ; Coronavirus Infections/immunology ; Enzyme-Linked Immunosorbent Assay/methods ; Female ; Flow Cytometry/methods ; France/epidemiology ; Healthy Volunteers ; Humans ; Immunoprecipitation/methods ; Luciferases ; Male ; Middle Aged ; Neutralization Tests ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/epidemiology ; Pneumonia, Viral/immunology ; SARS-CoV-2 ; Seroepidemiologic Studies ; Serologic Tests/methods ; Spike Glycoprotein, Coronavirus/immunology ; Translational Research, Biomedical ; Young Adult
    Chemische Substanzen Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Luciferases (EC 1.13.12.-)
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-08-17
    Erscheinungsland United States
    Dokumenttyp Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study
    ZDB-ID 2518854-9
    ISSN 1946-6242 ; 1946-6234
    ISSN (online) 1946-6242
    ISSN 1946-6234
    DOI 10.1126/scitranslmed.abc3103
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 6941: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  6. Artikel ; Online: SARS-CoV-2 serological analysis of COVID-19 hospitalized patients, pauci-symptomatic individuals and blood donors.

    Grzelak, Ludivine / Temmam, Sarah / Planchais, Cyril / Demeret, Caroline / Huon, Christele / Guivel, Florence / Staropoli, Isabelle / Chazal, Maxime / Dufloo, Jeremy / Planas, Delphine / Buchrieser, Julian / Rajah, Maaran Michael / Robinot, Remy / Porrot, Francoise / Albert, Melanie / Chen, Kuang-Yu / Crescenzo, Bernadette / Donati, Flora / Anna, Francois /
    Souque, Philippe / Gransagne, Marion / Bellalou, Jacques / Nowakowski, Mireille / Backovic, Marija / Bouadma, lila / Le Fevre, Lucie / Le Hingrat, Quentin / Descamps, Diane / Pourbaix, Anabelle / Yazdanpanah, Yazdan / Tondeur, Laura / Besombes, Camille / Ungeheuer, Marie-Noelle / Mellon, Guillaume / Morel, Pascal / Rolland, Simon / Rey, Felix / Behillil, Sylvie / Enouf, Vincent / Lemaitre, Audrey / Creach, Marie-Aude / Petres, Stephane / Escriou, Nicolas / Charneau, Pierre / Fontanet, Arnaud / Hoen, Bruno / Bruel, Timothee / Eloit, Marc / Mouquet, Hugo / Schwartz, Olivier / van der Werf, Sylvie

    medRxiv

    Abstract: It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their antibody response profile. Here, we performed a pilot study to assess the levels of anti-SARS-CoV-2 antibodies in ... ...

    Abstract It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their antibody response profile. Here, we performed a pilot study to assess the levels of anti-SARS-CoV-2 antibodies in samples taken from 491 pre- epidemic individuals, 51 patients from Hopital Bichat (Paris), 209 pauci-symptomatic individuals in the French Oise region and 200 contemporary Oise blood donors. Two in-house ELISA assays, that recognize the full-length nucleoprotein (N) or trimeric Spike (S) ectodomain were implemented. We also developed two novel assays: the S-Flow assay, which is based on the recognition of S at the cell surface by flow-cytometry, and the LIPS assay that recognizes diverse antigens (including S1 or N C- terminal domain) by immunoprecipitation. Overall, the results obtained with the four assays were similar, with differences in sensitivity that can be attributed to the technique and the antigen in use. High antibody titers were associated with neutralisation activity, assessed using infectious SARS-CoV- 2 or lentiviral-S pseudotypes. In hospitalized patients, seroconversion and neutralisation occurred on 5-14 days post symptom onset, confirming previous studies. Seropositivity was detected in 29% of pauci-symptomatic individuals within 15 days post-symptoms and 3 % of blood of healthy donors collected in the area of a cluster of COVID cases. Altogether, our assays allow for a broad evaluation of SARS-CoV2 seroprevalence and antibody profiling in different population subsets.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-04-24
    Verlag Cold Spring Harbor Laboratory Press
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2020.04.21.20068858
    Datenquelle COVID19

    Volltext online

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  7. Artikel: A comparison of four serological assays for detecting anti-SARS-CoV-2 antibodies in human serum samples from different populations

    Grzelak, Ludivine / Temmam, Sarah / Planchais, Cyril / Demeret, Caroline / Tondeur, Laura / Huon, Christèle / Guivel-Benhassine, Florence / Staropoli, Isabelle / Chazal, Maxime / Dufloo, Jeremy / Planas, Delphine / Buchrieser, Julian / Rajah, Maaran Michael / Robinot, Remy / Porrot, Françoise / Albert, Mélanie / Chen, Kuang-Yu / Crescenzo-Chaigne, Bernadette / Donati, Flora /
    Anna, François / Souque, Philippe / Gransagne, Marion / Bellalou, Jacques / Nowakowski, Mireille / Backovic, Marija / Bouadma, Lila / Le Fevre, Lucie / Le Hingrat, Quentin / Descamps, Diane / Pourbaix, Annabelle / Laouénan, Cédric / Ghosn, Jade / Yazdanpanah, Yazdan / Besombes, Camille / Jolly, Nathalie / Pellerin-Fernandes, Sandrine / Cheny, Olivia / Ungeheuer, Marie-Noëlle / Mellon, Guillaume / Morel, Pascal / Rolland, Simon / Rey, Felix A / Behillil, Sylvie / Enouf, Vincent / Lemaitre, Audrey / Créach, Marie-Aude / Petres, Stephane / Escriou, Nicolas / Charneau, Pierre / Fontanet, Arnaud

    Sci. transl. med

    Abstract: It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts ... ...

    Abstract It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts of anti-SARS-CoV-2 antibodies in serum samples obtained from 491 healthy individuals before the SARS-CoV-2 pandemic, 51 individuals hospitalized with COVID-19, 209 suspected cases of COVID-19 with mild symptoms, and 200 healthy blood donors. We used two ELISA assays that recognized the full-length nucleoprotein (N) or trimeric spike (S) protein ectodomain of SARS-CoV-2. In addition, we developed the S-Flow assay that recognized the S protein expressed at the cell surface using flow cytometry, and the luciferase immunoprecipitation system (LIPS) assay that recognized diverse SARS-CoV-2 antigens including the S1 domain and the carboxyl-terminal domain of N by immunoprecipitation. We obtained similar results with the four serological assays. Differences in sensitivity were attributed to the technique and the antigen used. High anti-SARS-CoV-2 antibody titers were associated with neutralization activity, which was assessed using infectious SARS-CoV-2 or lentiviral-S pseudotype virus. In hospitalized patients with COVID-19, seroconversion and virus neutralization occurred between 5 and 14 days after symptom onset, confirming previous studies. Seropositivity was detected in 32% of mildly symptomatic individuals within 15 days of symptom onset and in 3% of healthy blood donors. The four antibody assays that we used enabled a broad evaluation of SARS-CoV-2 seroprevalence and antibody profiling in different subpopulations within one region.
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #724557
    Datenquelle COVID19

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  8. Artikel ; Online: SARS-CoV-2 serological analysis of COVID-19 hospitalized patients, pauci-symptomatic individuals and blood donors.

    Grzelak, Ludivine / Temmam, Sarah / Planchais, Cyril / Demeret, Caroline / Huon, Christele / Guivel, Florence / Staropoli, Isabelle / Chazal, Maxime / Dufloo, Jeremy / Planas, Delphine / Buchrieser, Julian / Rajah, Maaran Michael / Robinot, Remy / Porrot, Francoise / Albert, Melanie / Chen, Kuang-Yu / Crescenzo, Bernadette / Donati, Flora / Anna, Francois /
    Souque, Philippe / Gransagne, Marion / Bellalou, Jacques / Nowakowski, Mireille / Backovic, Marija / Bouadma, lila / Le Fevre, Lucie / Le Hingrat, Quentin / Descamps, Diane / Pourbaix, Anabelle / Yazdanpanah, Yazdan / Tondeur, Laura / Besombes, Camille / Ungeheuer, Marie-Noelle / Mellon, Guillaume / Morel, Pascal / Rolland, Simon / Rey, Felix / Behillil, Sylvie / Enouf, Vincent / Lemaitre, Audrey / Creach, Marie-Aude / Petres, Stephane / Escriou, Nicolas / Charneau, Pierre / Fontanet, Arnaud / Hoen, Bruno / Bruel, Timothee / Eloit, Marc / Mouquet, Hugo / Schwartz, Olivier / van der Werf, Sylvie

    Abstract: It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their antibody response profile. Here, we performed a pilot study to assess the levels of anti-SARS-CoV-2 antibodies in ... ...

    Abstract It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their antibody response profile. Here, we performed a pilot study to assess the levels of anti-SARS-CoV-2 antibodies in samples taken from 491 pre- epidemic individuals, 51 patients from Hopital Bichat (Paris), 209 pauci-symptomatic individuals in the French Oise region and 200 contemporary Oise blood donors. Two in-house ELISA assays, that recognize the full-length nucleoprotein (N) or trimeric Spike (S) ectodomain were implemented. We also developed two novel assays: the S-Flow assay, which is based on the recognition of S at the cell surface by flow-cytometry, and the LIPS assay that recognizes diverse antigens (including S1 or N C- terminal domain) by immunoprecipitation. Overall, the results obtained with the four assays were similar, with differences in sensitivity that can be attributed to the technique and the antigen in use. High antibody titers were associated with neutralisation activity, assessed using infectious SARS-CoV- 2 or lentiviral-S pseudotypes. In hospitalized patients, seroconversion and neutralisation occurred on 5-14 days post symptom onset, confirming previous studies. Seropositivity was detected in 29% of pauci-symptomatic individuals within 15 days post-symptoms and 3 % of blood of healthy donors collected in the area of a cluster of COVID cases. Altogether, our assays allow for a broad evaluation of SARS-CoV2 seroprevalence and antibody profiling in different population subsets.
    Schlagwörter covid19
    Verlag MedRxiv; WHO
    Dokumenttyp Artikel ; Online
    DOI 10.1101/2020.04.21.20068858
    Datenquelle COVID19

    Kategorien

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

  9. Artikel ; Online: A comparison of four serological assays for detecting anti–SARS-CoV-2 antibodies in human serum samples from different populations

    Grzelak, Ludivine / Temmam, Sarah / Planchais, Cyril / Demeret, Caroline / Tondeur, Laura / Huon, Christèle / Guivel-Benhassine, Florence / Staropoli, Isabelle / Chazal, Maxime / Dufloo, Jeremy / Planas, Delphine / Buchrieser, Julian / Rajah, Maaran Michael / Robinot, Remy / Porrot, Françoise / Albert, Mélanie / Chen, Kuang-Yu / Crescenzo-Chaigne, Bernadette / Donati, Flora /
    Anna, François / Souque, Philippe / Gransagne, Marion / Bellalou, Jacques / Nowakowski, Mireille / Backovic, Marija / Bouadma, Lila / Le Fevre, Lucie / Le Hingrat, Quentin / Descamps, Diane / Pourbaix, Annabelle / Laouénan, Cédric / Ghosn, Jade / Yazdanpanah, Yazdan / Besombes, Camille / Jolly, Nathalie / Pellerin-Fernandes, Sandrine / Cheny, Olivia / Ungeheuer, Marie-Noëlle / Mellon, Guillaume / Morel, Pascal / Rolland, Simon / Rey, Felix A. / Behillil, Sylvie / Enouf, Vincent / Lemaitre, Audrey / Créach, Marie-Aude / Petres, Stephane / Escriou, Nicolas / Charneau, Pierre / Fontanet, Arnaud / Hoen, Bruno / Bruel, Timothée / Eloit, Marc / Mouquet, Hugo / Schwartz, Olivier / van der Werf, Sylvie

    Science Translational Medicine

    2020  Band 12, Heft 559, Seite(n) eabc3103

    Abstract: It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts ... ...

    Abstract It is of paramount importance to evaluate the prevalence of both asymptomatic and symptomatic cases of SARS-CoV-2 infection and their differing antibody response profiles. Here, we performed a pilot study of four serological assays to assess the amounts of anti–SARS-CoV-2 antibodies in serum samples obtained from 491 healthy individuals before the SARS-CoV-2 pandemic, 51 individuals hospitalized with COVID-19, 209 suspected cases of COVID-19 with mild symptoms, and 200 healthy blood donors. We used two ELISA assays that recognized the full-length nucleoprotein (N) or trimeric spike (S) protein ectodomain of SARS-CoV-2. In addition, we developed the S-Flow assay that recognized the S protein expressed at the cell surface using flow cytometry, and the luciferase immunoprecipitation system (LIPS) assay that recognized diverse SARS-CoV-2 antigens including the S1 domain and the carboxyl-terminal domain of N by immunoprecipitation. We obtained similar results with the four serological assays. Differences in sensitivity were attributed to the technique and the antigen used. High anti–SARS-CoV-2 antibody titers were associated with neutralization activity, which was assessed using infectious SARS-CoV-2 or lentiviral-S pseudotype virus. In hospitalized patients with COVID-19, seroconversion and virus neutralization occurred between 5 and 14 days after symptom onset, confirming previous studies. Seropositivity was detected in 32% of mildly symptomatic individuals within 15 days of symptom onset and in 3% of healthy blood donors. The four antibody assays that we used enabled a broad evaluation of SARS-CoV-2 seroprevalence and antibody profiling in different subpopulations within one region.
    Schlagwörter General Medicine ; covid19
    Sprache Englisch
    Verlag American Association for the Advancement of Science (AAAS)
    Erscheinungsland us
    Dokumenttyp Artikel ; Online
    ZDB-ID 2518854-9
    ISSN 1946-6242 ; 1946-6234
    ISSN (online) 1946-6242
    ISSN 1946-6234
    DOI 10.1126/scitranslmed.abc3103
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 6941: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

  10. Artikel: Switched on to mini grids

    Rolland, Simon

    Renewable energy focus

    Band v. 12,, Heft no. 3

    Abstract: A BILLION AND a half people around the world still don't have access to electricity. Could hybrid mini grids be the best way to provide electricity – particularly to rural, often isolated, areas? ...

    Abstract A BILLION AND a half people around the world still don't have access to electricity. Could hybrid mini grids be the best way to provide electricity – particularly to rural, often isolated, areas?
    Schlagwörter hybrids ; electricity ; people
    Sprache Englisch
    Dokumenttyp Artikel
    ISSN 1755-0084
    Datenquelle AGRIS - International Information System for the Agricultural Sciences and Technology

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

    Fernleihe an ZB MED

    Sie können sich den gewünschten Titel als lokale Nutzerin oder lokaler Nutzer von ZB MED direkt an den Standort Köln schicken lassen.

Zum Seitenanfang