Article ; Online: Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli.
2017 Volume 24, Page(s) 52
Abstract: In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large ... ...
Title translation | Identification moléculaire des espèces de Trichinella par PCR multiplex : nouvel éclairage pour Trichinella murrelli. |
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Abstract | In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella murrelli shows two bands of 127 bp and 316 bp. However, a third band of 256 bp can occur. This band can lead to misidentification, since it is similar to the 253 bp band displayed by Trichinella britovi. BLAST analysis confirmed that the 256 bp band is from T. murrelli. The aim of this short note is to inform analysts that T. murrelli larvae may display either two- or three-band patterns. |
MeSH term(s) | Animals ; DNA, Helminth/chemistry ; DNA, Helminth/genetics ; DNA, Helminth/isolation & purification ; Electrophoresis, Agar Gel ; Female ; Larva/classification ; Larva/genetics ; Mice ; Multiplex Polymerase Chain Reaction ; Sequence Alignment ; Trichinella/classification ; Trichinella/genetics ; Trichinella/isolation & purification |
Chemical Substances | DNA, Helminth |
Language | English |
Publishing date | 2017-12-08 |
Publishing country | France |
Document type | Journal Article |
ZDB-ID | 1187629-3 |
ISSN | 1776-1042 ; 1252-607X |
ISSN (online) | 1776-1042 |
ISSN | 1252-607X |
DOI | 10.1051/parasite/2017053 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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