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  1. Article ; Online: Molecular identification of Trichinella species by multiplex PCR: new insight for Trichinella murrelli.

    Karadjian, Grégory / Heckmann, Aurélie / Rosa, Giuseppe La / Pozio, Edoardo / Boireau, Pascal / Vallée, Isabelle

    Parasite (Paris, France)

    2017  Volume 24, Page(s) 52

    Abstract: In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large ... ...

    Title translation Identification moléculaire des espèces de Trichinella par PCR multiplex : nouvel éclairage pour Trichinella murrelli.
    Abstract In order to identify Trichinella at the species level, the commonly used test is a multiplex PCR, allowing the discrimination of nine out of the twelve taxa described so far. This test is based on five primer pairs amplifying fragments of the large subunit rDNA. Each taxon produces one or two bands of different sizes, resulting in a specific band pattern. By multiplex PCR, Trichinella murrelli shows two bands of 127 bp and 316 bp. However, a third band of 256 bp can occur. This band can lead to misidentification, since it is similar to the 253 bp band displayed by Trichinella britovi. BLAST analysis confirmed that the 256 bp band is from T. murrelli. The aim of this short note is to inform analysts that T. murrelli larvae may display either two- or three-band patterns.
    MeSH term(s) Animals ; DNA, Helminth/chemistry ; DNA, Helminth/genetics ; DNA, Helminth/isolation & purification ; Electrophoresis, Agar Gel ; Female ; Larva/classification ; Larva/genetics ; Mice ; Multiplex Polymerase Chain Reaction ; Sequence Alignment ; Trichinella/classification ; Trichinella/genetics ; Trichinella/isolation & purification
    Chemical Substances DNA, Helminth
    Language English
    Publishing date 2017-12-08
    Publishing country France
    Document type Journal Article
    ZDB-ID 1187629-3
    ISSN 1776-1042 ; 1252-607X
    ISSN (online) 1776-1042
    ISSN 1252-607X
    DOI 10.1051/parasite/2017053
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Pleural omentalisation with en bloc ligation of the thoracic duct and pericardiectomy for idiopathic chylothorax in nine dogs and four cats

    Bussadori, Roberto / Provera, Alessandra / Martano, Marina / Morello, Emanuela / Gonzalo-Orden, Josè Manuel / Rosa, Giuseppe La / Stefano, Nicoli / Maria, Repetto Silvia / Sara, Zabarino / Buracco, Paolo

    The Veterinary Journal. 2011 May, v. 188, no. 2

    2011  

    Abstract: Conventional treatment of idiopathic chylothorax (IC) involves thoracic duct (TD) ligation (with/without lymphagiography) combined with subphrenic pericardiectomy. Nine dogs and four cats with IC, which received intrathoracic omentalisation with TD en ... ...

    Abstract Conventional treatment of idiopathic chylothorax (IC) involves thoracic duct (TD) ligation (with/without lymphagiography) combined with subphrenic pericardiectomy. Nine dogs and four cats with IC, which received intrathoracic omentalisation with TD en bloc ligation (not preceded by lymphangiography) and subphrenic pericardiectomy, were evaluated retrospectively. Seven of nine dogs and 3/4 cats were still alive and disease-free at the time of reporting (range 10–53 and 19–31months, respectively). Clinical signs of IC did not decrease after the first surgery in one cat and one dog; in another dog clinical signs recurred after 5months. Overall efficacy rate of this one-stage combined procedure was 77% (6months), 73% (12months), and 57% (24months). Where a second surgery was performed in case of failure, the success rate in dogs was 89% (6months) and 80% (24months). Addition of pleural omentalisation to TD en bloc ligation and subphrenic pericardiectomy does not seem to improve results when compared with published data and at present does not seem advisable as a first choice.
    Keywords cats ; chylothorax ; dogs ; surgery ; thoracic duct
    Language English
    Dates of publication 2011-05
    Size p. 234-236.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 428614-5
    ISSN 1532-2971 ; 0372-5545 ; 1090-0233
    ISSN (online) 1532-2971
    ISSN 0372-5545 ; 1090-0233
    DOI 10.1016/j.tvjl.2010.05.010
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Chondroitin sulfate proteoglycan-4: A biomarker and a potential immunotherapeutic target for canine malignant melanoma

    Mayayo, Saray Lorda / Prestigio, Simone / Maniscalco, Lorella / Rosa, Giuseppe La / Aricò, Arianna / Maria, Raffaella De / Cavallo, Federica / Ferrone, Soldano / Buracco, Paolo / Iussich, Selina

    The Veterinary Journal. 2011 Nov., v. 190, no. 2

    2011  

    Abstract: Chondroitin sulfate proteoglycan-4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a membrane-bound chondroitin sulfate proteoglycan highly expressed by human melanoma cells. This phylogenetically conserved tumour ... ...

    Abstract Chondroitin sulfate proteoglycan-4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a membrane-bound chondroitin sulfate proteoglycan highly expressed by human melanoma cells. This phylogenetically conserved tumour antigen plays an important biological role in human melanoma, where it is used as a marker to diagnose forms with unusual characteristics, such as desmoplastic melanoma, and to detect melanoma cells in lymph nodes and peripheral blood, and as a target for immunotherapy because of its restricted distribution in normal tissues. To identify suitable targets to develop novel approaches of treating canine melanoma, CSPG4 was studies to see whether it is expressed in canine malignant melanomas. Immunohistochemical staining of 65 canine malignant melanomas with an anti-human CSPG4-specific antibody detected CSPG4 in 37 cases (56.9%). Positive staining was more frequent, albeit not significantly, in amelanotic compared to melanotic tumours and was statistically associated with tumours having both melanin and the epithelioid histotype. The frequency of CSPG4 expression was similar to that of other melanoma antigens used as diagnostic markers for canine malignant melanoma, such as Melan A and the protein recognized by the PNL2 monoclonal antibody. The results suggest that CSPG4 constitutes a new potential immunohistochemical marker of canine malignant melanoma and may represent an immunotherapeutic target as in humans.
    Keywords antigens ; biomarkers ; blood ; chondroitin sulfate ; dogs ; humans ; immunohistochemistry ; immunotherapy ; lymph nodes ; melanin ; melanoma ; monoclonal antibodies ; phylogeny ; proteoglycans
    Language English
    Dates of publication 2011-11
    Size p. e26-e30.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 428614-5
    ISSN 1532-2971 ; 0372-5545 ; 1090-0233
    ISSN (online) 1532-2971
    ISSN 0372-5545 ; 1090-0233
    DOI 10.1016/j.tvjl.2011.02.020
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: Chondroitin sulfate proteoglycan-4: a biomarker and a potential immunotherapeutic target for canine malignant melanoma.

    Mayayo, Saray Lorda / Prestigio, Simone / Maniscalco, Lorella / Rosa, Giuseppe La / Aricò, Arianna / Maria, Raffaella De / Cavallo, Federica / Ferrone, Soldano / Buracco, Paolo / Iussich, Selina

    Veterinary journal (London, England : 1997)

    2011  Volume 190, Issue 2, Page(s) e26–e30

    Abstract: Chondroitin sulfate proteoglycan-4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a membrane-bound chondroitin sulfate proteoglycan highly expressed by human melanoma cells. This phylogenetically conserved tumour ... ...

    Abstract Chondroitin sulfate proteoglycan-4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a membrane-bound chondroitin sulfate proteoglycan highly expressed by human melanoma cells. This phylogenetically conserved tumour antigen plays an important biological role in human melanoma, where it is used as a marker to diagnose forms with unusual characteristics, such as desmoplastic melanoma, and to detect melanoma cells in lymph nodes and peripheral blood, and as a target for immunotherapy because of its restricted distribution in normal tissues. To identify suitable targets to develop novel approaches of treating canine melanoma, CSPG4 was studies to see whether it is expressed in canine malignant melanomas. Immunohistochemical staining of 65 canine malignant melanomas with an anti-human CSPG4-specific antibody detected CSPG4 in 37 cases (56.9%). Positive staining was more frequent, albeit not significantly, in amelanotic compared to melanotic tumours and was statistically associated with tumours having both melanin and the epithelioid histotype. The frequency of CSPG4 expression was similar to that of other melanoma antigens used as diagnostic markers for canine malignant melanoma, such as Melan A and the protein recognized by the PNL2 monoclonal antibody. The results suggest that CSPG4 constitutes a new potential immunohistochemical marker of canine malignant melanoma and may represent an immunotherapeutic target as in humans.
    MeSH term(s) Animals ; Antigens/metabolism ; Biomarkers, Tumor/metabolism ; Dog Diseases/metabolism ; Dog Diseases/pathology ; Dogs ; Female ; Male ; Melanoma/metabolism ; Melanoma/pathology ; Melanoma/veterinary ; Mouth Neoplasms/metabolism ; Mouth Neoplasms/pathology ; Mouth Neoplasms/veterinary ; Neoplasm Staging/veterinary ; Proteoglycans/metabolism
    Chemical Substances Antigens ; Biomarkers, Tumor ; Proteoglycans ; chondroitin sulfate proteoglycan 4
    Language English
    Publishing date 2011-04-08
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 428614-5
    ISSN 1532-2971 ; 0372-5545 ; 1090-0233
    ISSN (online) 1532-2971
    ISSN 0372-5545 ; 1090-0233
    DOI 10.1016/j.tvjl.2011.02.020
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Organization and Activity of Italian Echocardiographic Laboratories: A Survey of the Italian Society of Echocardiography and Cardiovascular Imaging.

    Ciampi, Quirino / Pepi, Mauro / Antonini-Canterin, Francesco / Barbieri, Andrea / Barchitta, Agata / Faganello, Giorgio / Miceli, Sofia / Parato, Vito Maurizio / Tota, Antonio / Trocino, Giuseppe / Abbate, Massimiliana / Accadia, Maria / Alemanni, Rossella / Angelini, Andrea / Anglano, Francesco / Anselmi, Maurizio / Aquila, Iolanda / Aramu, Simona / Avogadri, Enrico /
    Azzaro, Giuseppe / Badano, Luigi / Balducci, Anna / Ballocca, Flavia / Barbarossa, Alessandro / Barbati, Giovanni / Barletta, Valentina / Barone, Daniele / Becherini, Francesco / Benfari, Giovanni / Beraldi, Monica / Bergandi, Gianluigi / Bilardo, Giuseppe / Binno, Simone Maurizio / Bolognesi, Massimo / Bongiovi, Stefano / Bragato, Renato Maria / Braggion, Gabriele / Brancaleoni, Rossella / Bursi, Francesca / Dessalvi, Christian Cadeddu / Cameli, Matteo / Canu, Antonella / Capitelli, Mariano / Capra, Anna Clara Maria / Carbonara, Rosa / Carbone, Maria / Carbonella, Marco / Carrabba, Nazario / Casavecchia, Grazia / Casula, Margherita / Chesi, Elena / Cicco, Sebastiano / Citro, Rodolfo / Cocchia, Rosangela / Colombo, Barbara Maria / Colonna, Paolo / Conte, Maddalena / Corrado, Giovanni / Cortesi, Pietro / Cortigiani, Lauro / Costantino, Marco Fabio / Cozza, Fabiana / Cucchini, Umberto / D'Angelo, Myriam / Ros, Santina Da / D'Andrea, Fabrizio / D'Andrea, Antonello / D'Auria, Francesca / De Caridi, Giovanni / De Feo, Stefania / De Matteis, Giovanni Maria / De Vecchi, Simona / Giudice, Carmen Del / Dell'Angela, Luca / Paoli, Lucrezia Delli / Dentamaro, Ilaria / Destefanis, Paola / Di Fulvio, Maria / Di Gaetano, Renato / Di Giannuario, Giovanna / Di Gioia, Angelo / Di Martino, Luigi Flavio Massimiliano / Di Muro, Carmine / Di Nora, Concetta / Di Salvo, Giovanni / Dodi, Claudio / Dogliani, Sarah / Donati, Federica / Dottori, Melissa / Epifani, Giuseppe / Fabiani, Iacopo / Ferrara, Francesca / Ferrara, Luigi / Ferrua, Stefania / Filice, Gemma / Fiorino, Maria / Forno, Davide / Garini, Alberto / Giarratana, Gioachino Agostino / Gigantino, Giuseppe / Giorgi, Mauro / Giubertoni, Elisa / Greco, Cosimo Angelo / Grigolato, Michele / Marra, Walter Grosso / Holzl, Anna / Iaiza, Alessandra / Iannaccone, Andrea / Ilardi, Federica / Imbalzano, Egidio / Inciardi, Riccardo / Inserra, Corinna Antonia / Iori, Emilio / Izzo, Annibale / Rosa, Giuseppe La / Labanti, Graziana / Lanzone, Alberto Maria / Lanzoni, Laura / Lapetina, Ornella / Leiballi, Elisa / Librera, Mariateresa / Conte, Carmenita Lo / Monaco, Maria Lo / Lombardo, Antonella / Luciani, Michelangelo / Lusardi, Paola / Magnante, Antonio / Malagoli, Alessandro / Malatesta, Gelsomina / Mancusi, Costantino / Manes, Maria Teresa / Manganelli, Fiore / Mantovani, Francesca / Manuppelli, Vincenzo / Marchese, Valeria / Marinacci, Lina / Mattioli, Roberto / Maurizio, Civelli / Mazza, Giuseppe Antonio / Mazza, Stefano / Melis, Marco / Meloni, Giulia / Merli, Elisa / Milan, Alberto / Minardi, Giovanni / Monaco, Antonella / Monte, Ines / Montresor, Graziano / Moreo, Antonella / Mori, Fabio / Morini, Sofia / Moro, Claudio / Morrone, Doralisa / Negri, Francesco / Nipote, Carmelo / Nisi, Fulvio / Nocco, Silvio / Novello, Luigi / Nunziata, Luigi / Perini, Alessandro Paoletti / Parodi, Antonello / Pasanisi, Emilio Maria / Pastorini, Guido / Pavasini, Rita / Pavoni, Daisy / Pedone, Chiara / Pelliccia, Francesco / Pelliciari, Giovanni / Pelloni, Elisa / Pergola, Valeria / Perillo, Giovanni / Petruccelli, Enrica / Pezzullo, Chiara / Piacentini, Gerardo / Picardi, Elisa / Pinna, Giovanni / Pizzarelli, Massimiliano / Pizzuti, Alfredo / Poggi, Matteo Maria / Posteraro, Alfredo / Privitera, Carmen / Rampazzo, Debora / Ratti, Carlo / Rettegno, Sara / Ricci, Fabrizio / Ricci, Caterina / Rolando, Cristina / Rossi, Stefania / Rovera, Chiara / Ruggieri, Roberta / Russo, Maria Giovanna / Sacchi, Nicola / Saladino, Antonino / Sani, Francesca / Sartori, Chiara / Scarabeo, Virginia / Sciacqua, Angela / Scillone, Antonio / Scopelliti, Pasquale Antonio / Scorza, Alfredo / Scozzafava, Angela / Serafini, Francesco / Serra, Walter / Severino, Sergio / Simeone, Beatrice / Sirico, Domenico / Solari, Marco / Spadaro, Gian Luca / Stefani, Laura / Strangio, Antonio / Surace, Francesca Chiara / Tamborini, Gloria / Tarquinio, Nicola / Tassone, Eliezer Joseph / Tavarozzi, Isabella / Tchana, Bertrand / Tedesco, Giuseppe / Tinto, Monica / Torzillo, Daniela / Totaro, Antonio / Triolo, Oreste Fabio / Troisi, Federica / Tusa, Maurizio / Vancheri, Federico / Varasano, Vincenzo / Venezia, Amedeo / Vermi, Anna Chiara / Villari, Bruno / Zampi, Giordano / Zannoni, Jessica / Zito, Concetta / Zugaro, Antonello / Di Bella, Gianluca / Carerj, Scipione

    Journal of cardiovascular echography

    2023  Volume 33, Issue 1, Page(s) 1–9

    Abstract: Background: The Italian Society of Echocardiography and Cardiovascular Imaging (SIECVI) conducted a national survey to understand better how different echocardiographic modalities are used and accessed in Italy.: Methods: We analyzed echocardiography ...

    Abstract Background: The Italian Society of Echocardiography and Cardiovascular Imaging (SIECVI) conducted a national survey to understand better how different echocardiographic modalities are used and accessed in Italy.
    Methods: We analyzed echocardiography laboratory activities over a month (November 2022). Data were retrieved via an electronic survey based on a structured questionnaire, uploaded on the SIECVI website.
    Results: Data were obtained from 228 echocardiographic laboratories: 112 centers (49%) in the northern, 43 centers (19%) in the central, and 73 (32%) in the southern regions. During the month of observation, we collected 101,050 transthoracic echocardiography (TTE) examinations performed in all centers. As concern other modalities there were performed 5497 transesophageal echocardiography (TEE) examinations in 161/228 centers (71%); 4057 stress echocardiography (SE) examinations in 179/228 centers (79%); and examinations with ultrasound contrast agents (UCAs) in 151/228 centers (66%). We did not find significant regional variations between the different modalities. The usage of picture archiving and communication system (PACS) was significantly higher in the northern (84%) versus central (49%) and southern (45%) centers (
    Conclusions: This nationwide survey demonstrated that digital infrastructures and advanced echocardiography modalities, such as 3D and STE, are widely available in Italy with a notable diffuse uptake of LUS in the core TTE examination, a suboptimal diffusion of PACS recording, and conservative use of UCA, 3D, and strain. There are significant differences between northern and central-southern regions and echocardiographic laboratories that pertain to the cardiac unit. This inhomogeneous distribution of technology represents one of the main issues that must be solved to standardize the practice of echocardiography.
    Language English
    Publishing date 2023-05-29
    Publishing country India
    Document type Journal Article
    ZDB-ID 2734045-4
    ISSN 2347-193X ; 2211-4122
    ISSN (online) 2347-193X
    ISSN 2211-4122
    DOI 10.4103/jcecho.jcecho_16_23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Chondroitin sulfate proteoglycan-4: A biomarker and a potential immunotherapeutic target for canine malignant melanoma

    Mayayo, Saray Lorda / Prestigio, Simone / Maniscalco, Lorella / Rosa, Giuseppe La / Aricò, Arianna / Maria, Raffaella De / Cavallo, Federica / Ferrone, Soldano / Buracco, Paolo / Iussich, Selina

    The Veterinary Journal

    Volume v. 190,, Issue no. 2

    Abstract: Chondroitin sulfate proteoglycan-4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a membrane-bound chondroitin sulfate proteoglycan highly expressed by human melanoma cells. This phylogenetically conserved tumour ... ...

    Abstract Chondroitin sulfate proteoglycan-4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a membrane-bound chondroitin sulfate proteoglycan highly expressed by human melanoma cells. This phylogenetically conserved tumour antigen plays an important biological role in human melanoma, where it is used as a marker to diagnose forms with unusual characteristics, such as desmoplastic melanoma, and to detect melanoma cells in lymph nodes and peripheral blood, and as a target for immunotherapy because of its restricted distribution in normal tissues. To identify suitable targets to develop novel approaches of treating canine melanoma, CSPG4 was studies to see whether it is expressed in canine malignant melanomas. Immunohistochemical staining of 65 canine malignant melanomas with an anti-human CSPG4-specific antibody detected CSPG4 in 37 cases (56.9%). Positive staining was more frequent, albeit not significantly, in amelanotic compared to melanotic tumours and was statistically associated with tumours having both melanin and the epithelioid histotype. The frequency of CSPG4 expression was similar to that of other melanoma antigens used as diagnostic markers for canine malignant melanoma, such as Melan A and the protein recognized by the PNL2 monoclonal antibody. The results suggest that CSPG4 constitutes a new potential immunohistochemical marker of canine malignant melanoma and may represent an immunotherapeutic target as in humans.
    Keywords melanin ; dogs ; immunohistochemistry ; phylogeny ; immunotherapy ; blood ; humans ; melanoma ; monoclonal antibodies ; biomarkers ; chondroitin sulfate ; proteoglycans ; lymph nodes ; antigens
    Language English
    Document type Article
    ISSN 1090-0233
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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  7. Article: Pleural omentalisation with en bloc ligation of the thoracic duct and pericardiectomy for idiopathic chylothorax in nine dogs and four cats

    Bussadori, Roberto / Provera, Alessandra / Martano, Marina / Morello, Emanuela / Gonzalo-Orden, Josè Manuel / Rosa, Giuseppe La / Stefano, Nicoli / Maria, Repetto Silvia / Sara, Zabarino / Buracco, Paolo

    The Veterinary Journal

    Volume v. 188,, Issue no. 2

    Abstract: Conventional treatment of idiopathic chylothorax (IC) involves thoracic duct (TD) ligation (with/without lymphagiography) combined with subphrenic pericardiectomy. Nine dogs and four cats with IC, which received intrathoracic omentalisation with TD en ... ...

    Abstract Conventional treatment of idiopathic chylothorax (IC) involves thoracic duct (TD) ligation (with/without lymphagiography) combined with subphrenic pericardiectomy. Nine dogs and four cats with IC, which received intrathoracic omentalisation with TD en bloc ligation (not preceded by lymphangiography) and subphrenic pericardiectomy, were evaluated retrospectively. Seven of nine dogs and 3/4 cats were still alive and disease-free at the time of reporting (range 10–53 and 19–31months, respectively). Clinical signs of IC did not decrease after the first surgery in one cat and one dog; in another dog clinical signs recurred after 5months. Overall efficacy rate of this one-stage combined procedure was 77% (6months), 73% (12months), and 57% (24months). Where a second surgery was performed in case of failure, the success rate in dogs was 89% (6months) and 80% (24months). Addition of pleural omentalisation to TD en bloc ligation and subphrenic pericardiectomy does not seem to improve results when compared with published data and at present does not seem advisable as a first choice.
    Keywords cats ; dogs ; chylothorax ; thoracic duct ; surgery
    Language English
    Document type Article
    ISSN 1090-0233
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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