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  1. Artikel ; Online: Assessment of the microbiological quality of natural mineral waters according to the manufacturing time of 20 L returnable packs in Brazil.

    Luz, Isabelle da Silva / Vasconcellos, Luiza / Medeiros, Valéria de Mello / Miranda, Catia Aparecida Chaia / Rosas, Carla de Oliveira / Pimenta, Márcia Maria Araújo / Ferreira, Fernando César / Romão, Célia Maria Carvalho Pereira Araújo / Brandão, Marcelo Luiz Lima / Miagostovich, Marize Pereira

    FEMS microbiology letters

    2020  Band 367, Heft 15

    Abstract: This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, ... ...

    Abstract This study aimed to assess the microbiological quality of natural mineral waters commercialized in 20 L returnable packs in Brazil by investigating the presence of bacteria and viruses in packs with different manufacturing times (Tm). With this purpose, 99 water samples from 33 lots (n = 3/batch) of 15 brands, obtained from packs with three intervals of Tm, were analyzed. Total coliforms (16.2%), Pseudomonas aeruginosa (9.9%), sulphite-reducing Clostridium (5.0%) and Escherichia coli (2.0%) were detected but enterococci and norovirus GII not. Regarding brands, 11 (73.3%) presented unsatisfactory results for at least one of the lots analyzed. Pseudomonas aeruginosa analysis revealed six sequence types and strains were susceptible to all antibiotics tested and were able to produce biofilms. Human adenovirus (4) and norovirus GI (9) were also identified in nine samples randomly selected. Natural mineral waters commercialized in 20 L packs with Tm ≥ 2 years presented more microbiological contamination (P ≤ 0.012) than ones with a Tm of 0-1 year or a Tm of 1-2 years. These results suggest that the validity period of reusable 20 L packs should be reduced or that they can no longer be reused.
    Mesh-Begriff(e) Bacteria/classification ; Bacteria/isolation & purification ; Brazil ; Mineral Waters/microbiology ; Mineral Waters/virology ; Time Factors ; Viruses/isolation & purification ; Water Microbiology
    Chemische Substanzen Mineral Waters
    Sprache Englisch
    Erscheinungsdatum 2020-07-16
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 752343-9
    ISSN 1574-6968 ; 0378-1097
    ISSN (online) 1574-6968
    ISSN 0378-1097
    DOI 10.1093/femsle/fnaa120
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Evaluation of skimmed milk flocculation method for virus recovery from tomatoes

    Melgaço, Fabiana Gil / Corrêa, Adriana Abreu / Ganime, Ana Carolina / Brandão, Marcelo Luiz Lima / Medeiros, Valéria de Mello / Rosas, Carla de Oliveira / Lopes, Silvia Maria dos Reis / Miagostovich, Marize Pereira

    Sociedade Brasileira de Microbiologia Brazilian journal of microbiology. 2018 Nov., v. 49

    2018  

    Abstract: This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) ... ...

    Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.
    Schlagwörter Adenoviridae ; Norovirus ; Salmonella ; Solanum lycopersicum var. cerasiforme ; cherries ; cherry tomatoes ; fecal bacteria ; flocculation ; food safety ; grape tomatoes ; humans ; monitoring ; process control ; skim milk ; viruses ; Brazil
    Sprache Englisch
    Erscheinungsverlauf 2018-11
    Umfang p. 34-39.
    Erscheinungsort Elsevier Editora Ltda.
    Dokumenttyp Artikel
    ZDB-ID 2042078-X
    ISSN 1517-8382
    ISSN 1517-8382
    DOI 10.1016/j.bjm.2018.04.014
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel ; Online: Evaluation of skimmed milk flocculation method for virus recovery from tomatoes.

    Melgaço, Fabiana Gil / Corrêa, Adriana Abreu / Ganime, Ana Carolina / Brandão, Marcelo Luiz Lima / Medeiros, Valéria de Mello / Rosas, Carla de Oliveira / Lopes, Silvia Maria Dos Reis / Miagostovich, Marize Pereira

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2018  Band 49 Suppl 1, Seite(n) 34–39

    Abstract: This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) ... ...

    Abstract This study aimed to evaluate the elution-concentration methodology based on skimmed milk flocculation from three varieties of tomatoes (Solanum lycopersicum L. [globe], Solanum lycopersicum var. cerasiforme [cherry] and hybrid cocktail [grape tomato]) for further monitoring of field samples. Spiking experiments were performed to determine the success rate and efficiency recovery of human norovirus (NoV) genogroup II, norovirus murine-1 (MNV-1) used as sample process control virus and human adenovirus (HAdV). Mean values of 18.8%, 2.8% and 44.0% were observed for NoV GII, MNV-1 and HAdV, respectively with differences according to the types of tomatoes, with lower efficiency for cherry tomatoes. Analysis of 90 samples, obtained at commercial establishments in the metropolitan region of Rio de Janeiro State, revealed 4.5% positivity for HAdV. Bacterial analysis was also performed with no detection of Salmonella spp., L. monocytogenes and fecal coliforms. Data demonstrated that the skimmed milk flocculation method is suitable for recovering HAdV from tomatoes and highlights the need for considering investigation in order to improve food safety.
    Mesh-Begriff(e) Animals ; Cattle ; Flocculation ; Food Microbiology/instrumentation ; Food Microbiology/methods ; Fruit/chemistry ; Fruit/classification ; Fruit/virology ; Lycopersicon esculentum/chemistry ; Lycopersicon esculentum/classification ; Lycopersicon esculentum/virology ; Milk/chemistry ; Viruses/classification ; Viruses/genetics ; Viruses/isolation & purification
    Sprache Englisch
    Erscheinungsdatum 2018-08-16
    Erscheinungsland Brazil
    Dokumenttyp Evaluation Studies ; Journal Article
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1517-8382
    ISSN (online) 1678-4405
    ISSN 1517-8382
    DOI 10.1016/j.bjm.2018.04.014
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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