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  1. Artikel ; Online: Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort.

    Smith, Muneerah / Abdesselem, Houari B / Mullins, Michelle / Tan, Ti-Myen / Nel, Andrew J M / Al-Nesf, Maryam A Y / Bensmail, Ilham / Majbour, Nour K / Vaikath, Nishant N / Naik, Adviti / Ouararhni, Khalid / Mohamed-Ali, Vidya / Al-Maadheed, Mohammed / Schell, Darien T / Baros-Steyl, Seanantha S / Anuar, Nur D / Ismail, Nur H / Morris, Priscilla E / Mamat, Raja N R /
    Rosli, Nurul S M / Anwar, Arif / Ellan, Kavithambigai / Zain, Rozainanee M / Burgers, Wendy A / Mayne, Elizabeth S / El-Agnaf, Omar M A / Blackburn, Jonathan M

    Viruses

    2021  Band 13, Heft 5

    Abstract: The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response ... ...

    Abstract The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100% sensitivity and specificity) in a case-control cohort (
    Sprache Englisch
    Erscheinungsdatum 2021-04-28
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13050786
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Age, Disease Severity and Ethnicity Influence Humoral Responses in a Multi-Ethnic COVID-19 Cohort

    Smith, Muneerah / Abdesselem, Houari B / Mullins, Michelle / Tan, Ti-Myen / Nel, Andrew J. M / Al-Nesf, Maryam A. Y / Bensmail, Ilham / Majbour, Nour K / Vaikath, Nishant N / Naik, Adviti / Ouararhni, Khalid / Mohamed-Ali, Vidya / Al-Maadheed, Mohammed / Schell, Darien T / Baros-Steyl, Seanantha S / Anuar, Nur D / Ismail, Nur H / Morris, Priscilla E / Mamat, Raja N. R /
    Rosli, Nurul S. M / Anwar, Arif / Ellan, Kavithambigai / Zain, Rozainanee M / Burgers, Wendy A / Mayne, Elizabeth S / El-Agnaf, Omar M. A / Blackburn, Jonathan M

    Viruses. 2021 Apr. 28, v. 13, no. 5

    2021  

    Abstract: The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response ... ...

    Abstract The COVID-19 pandemic has affected all individuals across the globe in some way. Despite large numbers of reported seroprevalence studies, there remains a limited understanding of how the magnitude and epitope utilization of the humoral immune response to SARS-CoV-2 viral anti-gens varies within populations following natural infection. Here, we designed a quantitative, multi-epitope protein microarray comprising various nucleocapsid protein structural motifs, including two structural domains and three intrinsically disordered regions. Quantitative data from the microarray provided complete differentiation between cases and pre-pandemic controls (100% sensitivity and specificity) in a case-control cohort (n = 100). We then assessed the influence of disease severity, age, and ethnicity on the strength and breadth of the humoral response in a multi-ethnic cohort (n = 138). As expected, patients with severe disease showed significantly higher antibody titers and interestingly also had significantly broader epitope coverage. A significant increase in antibody titer and epitope coverage was observed with increasing age, in both mild and severe disease, which is promising for vaccine efficacy in older individuals. Additionally, we observed significant differences in the breadth and strength of the humoral immune response in relation to ethnicity, which may reflect differences in genetic and lifestyle factors. Furthermore, our data enabled localization of the immuno-dominant epitope to the C-terminal structural domain of the viral nucleocapsid protein in two independent cohorts. Overall, we have designed, validated, and tested an advanced serological assay that enables accurate quantitation of the humoral response post natural infection and that has revealed unexpected differences in the magnitude and epitope utilization within a population.
    Schlagwörter COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; antibodies ; disease severity ; epitopes ; humoral immunity ; immunologic techniques ; lifestyle ; nationalities and ethnic groups ; nucleocapsid proteins ; protein microarrays ; seroprevalence ; vaccines
    Sprache Englisch
    Erscheinungsverlauf 2021-0428
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v13050786
    Datenquelle NAL Katalog (AGRICOLA)

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    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

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