Artikel: Comparative evaluation of the QMAC-dRAST V2.0 system for rapid antibiotic susceptibility testing of Gram-negative blood culture isolates
Journal of microbiological methods. 2020 May, v. 172
2020
Abstract: To comparatively evaluate the performance of the rapid antimicrobial susceptibility testing (AST) system QMAC-dRAST V2.0 and of standard disk diffusion in agar, AST was performed directly from 100 positive blood culture bottles with Gram-negative bacilli. ...
Abstract | To comparatively evaluate the performance of the rapid antimicrobial susceptibility testing (AST) system QMAC-dRAST V2.0 and of standard disk diffusion in agar, AST was performed directly from 100 positive blood culture bottles with Gram-negative bacilli. AST results provided by QMAC-dRAST showed 92.9% agreement with disk diffusion method results. Discrepancies observed between results obtained with QMAC-dRAST and disk diffusion method conducted to 10 very major errors (0.8%, S with QMAC-dRAST vs R with disk diffusion method), 40 major errors (3.2%, R vs S, respectively), 15 minor errors (1.2%, S vs I or I vs R, respectively) and 23 very minors errors (1.8%, I vs S or R vs I, respectively). For very major and major errors, in only 36% of the cases did the repeat QMAC-dRAST confirm the initial result, whereas a repeat AST using disk diffusion method confirmed the initial result in 92% of cases. AST results obtained using microdilution in liquid medium confirmed those obtained with QMAC-dRAST and disk diffusion method in 4% and 89%, respectively. Repeatability and reproducibility tests performed on QMAC-dRAST using reference strains showed 94% to 100% of R/S/I categorical agreement. |
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Schlagwörter | Gram-negative bacteria ; agar ; antibiotic resistance ; blood ; bottles ; disk diffusion antimicrobial test |
Sprache | Englisch |
Erscheinungsverlauf | 2020-05 |
Erscheinungsort | Elsevier B.V. |
Dokumenttyp | Artikel |
ZDB-ID | 604916-3 |
ISSN | 1872-8359 ; 0167-7012 |
ISSN (online) | 1872-8359 |
ISSN | 0167-7012 |
DOI | 10.1016/j.mimet.2020.105902 |
Datenquelle | NAL Katalog (AGRICOLA) |
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