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  1. Artikel ; Online: Bacteriophage Infections of Biofilms of Health Care-Associated Pathogens:

    Santiago, Ariel J / Donlan, Rodney M

    EcoSal Plus

    2020  Band 9, Heft 1

    Abstract: Members of the ... ...

    Abstract Members of the family
    Mesh-Begriff(e) Anti-Bacterial Agents/therapeutic use ; Bacterial Infections/therapy ; Bacteriophages/pathogenicity ; Biofilms ; Cross Infection/microbiology ; Host Specificity ; Humans ; Klebsiella pneumoniae/virology ; Phage Therapy/methods
    Chemische Substanzen Anti-Bacterial Agents
    Sprache Englisch
    Erscheinungsdatum 2020-11-28
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2324-6200
    ISSN (online) 2324-6200
    DOI 10.1128/ecosalplus.ESP-0029-2019
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Supplemental nutrients stimulate the amplification of carbapenemase-producing

    Burgos-Garay, María L / Santiago, Ariel J / Kartforosh, Leila / Kotay, Shireen / Donlan, Rodney M

    Biofouling

    2021  Band 37, Heft 5, Seite(n) 465–480

    Abstract: Liquid wastes (LW) disposed in hospital handwashing sinks may affect colonization of sink P-traps by carbapenemase- ... ...

    Abstract Liquid wastes (LW) disposed in hospital handwashing sinks may affect colonization of sink P-traps by carbapenemase-producing
    Mesh-Begriff(e) Anti-Bacterial Agents/therapeutic use ; Bacterial Proteins ; Biofilms ; Humans ; Klebsiella Infections ; Klebsiella pneumoniae ; Nutrients ; beta-Lactamases
    Chemische Substanzen Anti-Bacterial Agents ; Bacterial Proteins ; beta-Lactamases (EC 3.5.2.6) ; carbapenemase (EC 3.5.2.6)
    Sprache Englisch
    Erscheinungsdatum 2021-07-01
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 1029-2454
    ISSN (online) 1029-2454
    DOI 10.1080/08927014.2021.1915998
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Bacteriophage treatment of carbapenemase-producing

    Santiago, Ariel J / Burgos-Garay, Maria L / Kartforosh, Leila / Mazher, Mustafa / Donlan, Rodney M

    AIMS microbiology

    2020  Band 6, Heft 1, Seite(n) 43–63

    Abstract: The p-traps of hospital handwashing sinks represent a potential reservoir for antimicrobial-resistant organisms of major public health concern, such as carbapenemase-producing KPC+ ...

    Abstract The p-traps of hospital handwashing sinks represent a potential reservoir for antimicrobial-resistant organisms of major public health concern, such as carbapenemase-producing KPC+
    Sprache Englisch
    Erscheinungsdatum 2020-02-26
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2471-1888
    ISSN (online) 2471-1888
    DOI 10.3934/microbiol.2020003
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Inhibition and Dispersal of Pseudomonas aeruginosa Biofilms by Combination Treatment with Escapin Intermediate Products and Hydrogen Peroxide.

    Santiago, Ariel J / Ahmed, Marwa N A / Wang, Shu-Lin / Damera, Krishna / Wang, Binghe / Tai, Phang C / Gilbert, Eric S / Derby, Charles D

    Antimicrobial agents and chemotherapy

    2016  Band 60, Heft 9, Seite(n) 5554–5562

    Abstract: Escapin is an l-amino acid oxidase that acts on lysine to produce hydrogen peroxide (H2O2), ammonia, and equilibrium mixtures of several organic acids collectively called escapin intermediate products (EIP). Previous work showed that the combination of ... ...

    Abstract Escapin is an l-amino acid oxidase that acts on lysine to produce hydrogen peroxide (H2O2), ammonia, and equilibrium mixtures of several organic acids collectively called escapin intermediate products (EIP). Previous work showed that the combination of synthetic EIP and H2O2 functions synergistically as an antimicrobial toward diverse planktonic bacteria. We initiated the present study to investigate how the combination of EIP and H2O2 affected bacterial biofilms, using Pseudomonas aeruginosa as a model. Specifically, we examined concentrations of EIP and H2O2 that inhibited biofilm formation or fostered disruption of established biofilms. High-throughput assays of biofilm formation using microtiter plates and crystal violet staining showed a significant effect from pairing EIP and H2O2, resulting in inhibition of biofilm formation relative to biofilm formation in untreated controls or with EIP or H2O2 alone. Similarly, flow cell analysis and confocal laser scanning microscopy revealed that the EIP and H2O2 combination reduced the biomass of established biofilms relative to that of the controls. Area layer analysis of biofilms posttreatment indicated that disruption of biomass occurs down to the substratum. Only nanomolar to micromolar concentrations of EIP and H2O2 were required to impact biofilm formation or disruption, and these concentrations are significantly lower than those causing bactericidal effects on planktonic bacteria. Micromolar concentrations of EIP and H2O2 combined enhanced P. aeruginosa swimming motility compared to the effect of either EIP or H2O2 alone. Collectively, our results suggest that the combination of EIP and H2O2 may affect biofilms by interfering with bacterial attachment and destabilizing the biofilm matrix.
    Mesh-Begriff(e) Anti-Bacterial Agents/pharmacology ; Biofilms/drug effects ; Hydrogen Peroxide/pharmacology ; L-Amino Acid Oxidase/pharmacology ; Pseudomonas aeruginosa/drug effects
    Chemische Substanzen Anti-Bacterial Agents ; Hydrogen Peroxide (BBX060AN9V) ; L-Amino Acid Oxidase (EC 1.4.3.2)
    Sprache Englisch
    Erscheinungsdatum 2016-09
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 217602-6
    ISSN 1098-6596 ; 0066-4804
    ISSN (online) 1098-6596
    ISSN 0066-4804
    DOI 10.1128/AAC.02984-15
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel: Constructing multispecies biofilms with defined compositions by sequential deposition of bacteria

    Stubblefield, Bryan A / Howery, Kristen E / Islam, Bianca N / Santiago, Ariel J / Cardenas, Wendy E / Gilbert, Eric S

    Applied microbiology and biotechnology. 2010 May, v. 86, no. 6

    2010  

    Abstract: Rationally-assembled multispecies biofilms could benefit applied processes including mixed waste biodegradation and drug biosynthesis by combining complementary metabolic pathways into single functional communities. We hypothesized that the cellular ... ...

    Abstract Rationally-assembled multispecies biofilms could benefit applied processes including mixed waste biodegradation and drug biosynthesis by combining complementary metabolic pathways into single functional communities. We hypothesized that the cellular composition of mature multispecies biofilms could be manipulated by controlling the number of each cell type present on newly colonized surfaces. To test this idea, we developed a method for attaching specific numbers of bacteria to a flow cell by recirculating cell suspensions. Initial work revealed a nonlinear relationship between suspension cell density and areal density when two strains of Escherichia coli were simultaneously recirculated; in contrast, sequential recirculation resulted in a predictable deposition of cell numbers. Quantitative analysis of cell distributions in 48-h biofilms comprised of the E. coli strains demonstrated a strong relationship between their distribution at the substratum and their presence in mature biofilms. Sequentially depositing E. coli with either Pseudomonas aeruginosa or Bacillus subtilis determined small but reproducible differences in the areal density of the second microorganism recirculated relative to its areal density when recirculated alone. Overall, the presented method offers a simple and reproducible way to construct multispecies biofilms with defined compositions for biocatalytic processes.
    Schlagwörter Bacillus subtilis ; Escherichia coli ; Pseudomonas aeruginosa ; bacteria ; biochemical pathways ; biofilm ; cell suspension culture ; quantitative analysis
    Sprache Englisch
    Erscheinungsverlauf 2010-05
    Umfang p. 1941-1946.
    Verlag Springer-Verlag
    Erscheinungsort Berlin/Heidelberg
    Dokumenttyp Artikel
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-010-2473-y
    Datenquelle NAL Katalog (AGRICOLA)

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  6. Artikel ; Online: Constructing multispecies biofilms with defined compositions by sequential deposition of bacteria.

    Stubblefield, Bryan A / Howery, Kristen E / Islam, Bianca N / Santiago, Ariel J / Cardenas, Wendy E / Gilbert, Eric S

    Applied microbiology and biotechnology

    2010  Band 86, Heft 6, Seite(n) 1941–1946

    Abstract: Rationally-assembled multispecies biofilms could benefit applied processes including mixed waste biodegradation and drug biosynthesis by combining complementary metabolic pathways into single functional communities. We hypothesized that the cellular ... ...

    Abstract Rationally-assembled multispecies biofilms could benefit applied processes including mixed waste biodegradation and drug biosynthesis by combining complementary metabolic pathways into single functional communities. We hypothesized that the cellular composition of mature multispecies biofilms could be manipulated by controlling the number of each cell type present on newly colonized surfaces. To test this idea, we developed a method for attaching specific numbers of bacteria to a flow cell by recirculating cell suspensions. Initial work revealed a nonlinear relationship between suspension cell density and areal density when two strains of Escherichia coli were simultaneously recirculated; in contrast, sequential recirculation resulted in a predictable deposition of cell numbers. Quantitative analysis of cell distributions in 48-h biofilms comprised of the E. coli strains demonstrated a strong relationship between their distribution at the substratum and their presence in mature biofilms. Sequentially depositing E. coli with either Pseudomonas aeruginosa or Bacillus subtilis determined small but reproducible differences in the areal density of the second microorganism recirculated relative to its areal density when recirculated alone. Overall, the presented method offers a simple and reproducible way to construct multispecies biofilms with defined compositions for biocatalytic processes.
    Mesh-Begriff(e) Bacillus subtilis/growth & development ; Bacillus subtilis/physiology ; Bacterial Adhesion ; Bacteriological Techniques ; Biofilms/growth & development ; Colony Count, Microbial ; Environmental Microbiology ; Escherichia coli/growth & development ; Escherichia coli/physiology ; Microbial Interactions ; Pseudomonas aeruginosa/growth & development ; Pseudomonas aeruginosa/physiology
    Sprache Englisch
    Erscheinungsdatum 2010-05
    Erscheinungsland Germany
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 392453-1
    ISSN 1432-0614 ; 0171-1741 ; 0175-7598
    ISSN (online) 1432-0614
    ISSN 0171-1741 ; 0175-7598
    DOI 10.1007/s00253-010-2473-y
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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