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  1. Artikel ; Online: 3D single cell scale anatomical map of sex-dependent variability of the rat intrinsic cardiac nervous system

    Clara Leung / Shaina Robbins / Alison Moss / Maci Heal / Mahyar Osanlouy / Richard Christie / Navid Farahani / Corey Monteith / Jin Chen / Peter Hunter / Susan Tappan / Rajanikanth Vadigepalli / Zixi (Jack) Cheng / James S. Schwaber

    iScience, Vol 24, Iss 7, Pp 102795- (2021)

    2021  

    Abstract: Summary: We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across four male and three female hearts. We find the ICNS has a reliable structural organizational plan across individuals that ... ...

    Abstract Summary: We developed and analyzed a single cell scale anatomical map of the rat intrinsic cardiac nervous system (ICNS) across four male and three female hearts. We find the ICNS has a reliable structural organizational plan across individuals that provide the foundation for further analyses of the ICNS in cardiac function and disease. The distribution of the ICNS was evaluated by 3D visualization and data-driven clustering. The pattern, distribution, and clustering of ICNS neurons across all male and female rat hearts is highly conserved, demonstrating a coherent organizational plan where distinct clusters of neurons are consistently localized. Female hearts had fewer neurons, lower packing density, and slightly reduced distribution, but with identical localization. We registered the anatomical data from each heart to a geometric scaffold, normalizing their 3D coordinates for standardization of common anatomical planes and providing a path where multiple experimental results and data types can be integrated and compared.
    Schlagwörter Biology of gender ; Cardiovascular medicine ; Imaging anatomy ; Systems neuroscience ; Science ; Q
    Thema/Rubrik (Code) 590
    Sprache Englisch
    Erscheinungsdatum 2021-07-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: A Comprehensive Integrated Anatomical and Molecular Atlas of Rat Intrinsic Cardiac Nervous System

    Sirisha Achanta / Jonathan Gorky / Clara Leung / Alison Moss / Shaina Robbins / Leonard Eisenman / Jin Chen / Susan Tappan / Maci Heal / Navid Farahani / Todd Huffman / Steve England / Zixi (Jack) Cheng / Rajanikanth Vadigepalli / James S. Schwaber

    iScience, Vol 23, Iss 6, Pp 101140- (2020)

    2020  

    Abstract: Summary: We have developed and integrated several technologies including whole-organ imaging and software development to support an initial precise 3D neuroanatomical mapping and molecular phenotyping of the intracardiac nervous system (ICN). While ... ...

    Abstract Summary: We have developed and integrated several technologies including whole-organ imaging and software development to support an initial precise 3D neuroanatomical mapping and molecular phenotyping of the intracardiac nervous system (ICN). While qualitative and gross anatomical descriptions of the anatomy of the ICN have each been pursued, we here bring forth a comprehensive atlas of the entire rat ICN at single-cell resolution. Our work precisely integrates anatomical and molecular data in the 3D digitally reconstructed whole heart with resolution at the micron scale. We now display the full extent and the position of neuronal clusters on the base and posterior left atrium of the rat heart, and the distribution of molecular phenotypes that are defined along the base-to-apex axis, which had not been previously described. The development of these approaches needed for this work has produced method pipelines that provide the means for mapping other organs.
    Schlagwörter Imaging Anatomy ; Small Animal Imaging ; Rodent Cardiology ; Molecular Neuroscience ; Cellular Neuroscience ; Transcriptomics ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2020-06-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel ; Online: A single cell transcriptomics map of paracrine networks in the intrinsic cardiac nervous system

    Alison Moss / Shaina Robbins / Sirisha Achanta / Lakshmi Kuttippurathu / Scott Turick / Sean Nieves / Peter Hanna / Elizabeth H. Smith / Donald B. Hoover / Jin Chen / Zixi (Jack) Cheng / Jeffrey L. Ardell / Kalyanam Shivkumar / James S. Schwaber / Rajanikanth Vadigepalli

    iScience, Vol 24, Iss 7, Pp 102713- (2021)

    2021  

    Abstract: Summary: We developed a spatially-tracked single neuron transcriptomics map of an intrinsic cardiac ganglion, the right atrial ganglionic plexus (RAGP) that is a critical mediator of sinoatrial node (SAN) activity. This 3D representation of RAGP used ... ...

    Abstract Summary: We developed a spatially-tracked single neuron transcriptomics map of an intrinsic cardiac ganglion, the right atrial ganglionic plexus (RAGP) that is a critical mediator of sinoatrial node (SAN) activity. This 3D representation of RAGP used neuronal tracing to extensively map the spatial distribution of the subset of neurons that project to the SAN. RNA-seq of laser capture microdissected neurons revealed a distinct composition of RAGP neurons compared to the central nervous system and a surprising finding that cholinergic and catecholaminergic markers are coexpressed, suggesting multipotential phenotypes that can drive neuroplasticity within RAGP. High-throughput qPCR of hundreds of laser capture microdissected single neurons confirmed these findings and revealed a high dimensionality of neuromodulatory factors that contribute to dynamic control of the heart. Neuropeptide-receptor coexpression analysis revealed a combinatorial paracrine neuromodulatory network within RAGP informing follow-on studies on the vagal control of RAGP to regulate cardiac function in health and disease.
    Schlagwörter Cardiovascular medicine ; Molecular physiology ; Systems neuroscience ; Transcriptomics ; Science ; Q
    Sprache Englisch
    Erscheinungsdatum 2021-07-01T00:00:00Z
    Verlag Elsevier
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

    Volltext online

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