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  1. Article ; Online: The effects of oxidative stress and intracellular calcium on mitochondrial permeability transition pore formation in equine spermatozoa.

    Gibb, Zamira / Aitken, Robert J / Sheridan, Alecia R / Holt, Brandan / Waugh, Stephanie / Swegen, Aleona

    FASEB bioAdvances

    2024  Volume 6, Issue 6, Page(s) 143–158

    Abstract: The in vitro storage of stallion spermatozoa for use in artificial insemination leads to oxidative stress and imbalances in calcium homeostasis that trigger the formation of the mitochondrial permeability transition pore (mPTP), resulting in premature ... ...

    Abstract The in vitro storage of stallion spermatozoa for use in artificial insemination leads to oxidative stress and imbalances in calcium homeostasis that trigger the formation of the mitochondrial permeability transition pore (mPTP), resulting in premature cell death. However, little is understood about the dynamics and the role of mPTP formation in mammalian spermatozoa. Here, we identify an important role for mPTP in stallion sperm Ca
    Language English
    Publishing date 2024-05-03
    Publishing country United States
    Document type Journal Article
    ISSN 2573-9832
    ISSN (online) 2573-9832
    DOI 10.1096/fba.2023-00051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Causative mechanisms and functional correlates of MTT reduction in stallion spermatozoa.

    Medica, Ashlee Jade / Gibb, Zamira / Sheridan, Alecia / Harrison, Natasha / Aitken, Robert John

    Reproduction (Cambridge, England)

    2022  Volume 163, Issue 6, Page(s) 341–350

    Abstract: MTT is a commonly used cell vitality probe, due to its ability to form insoluble formazan deposits at cellular locations of intense oxidoreductase activity. Although this response is considered a reflection of mitochondrial redox activity, extra- ... ...

    Abstract MTT is a commonly used cell vitality probe, due to its ability to form insoluble formazan deposits at cellular locations of intense oxidoreductase activity. Although this response is considered a reflection of mitochondrial redox activity, extra-mitochondrial sites of MTT reduction have been recognized within the spermatozoa of several mammalian species. Therefore, the aim of this study was to determine the major sites and causative mechanisms of MTT reduction in stallion spermatozoa. Our results show that stallion spermatozoa displayed substantial mitochondrial formazan deposition, as well as a single extra-mitochondrial formazan deposit in various locations on the sperm head in approximately 20% of cells. The quality and capacitation status of stallion spermatozoa were positively correlated with the presence of an extra-mitochondrial formazan granule. Additionally, extra-mitochondrial formazan deposition was suppressed by the presence of an NADPH oxidase (NOX) inhibitor (VAS2870; active against NOX2, NOX4 and NOX5), MnTMPyP (SOD mimetic) and zinc (NOX5 inhibitor) suggesting that extra-mitochondrial MTT reduction may be facilitated by NOX-mediated ROS generating activity, conceivably NOX5 or NOX2. When comparing MTT to resazurin, another well-known probe used to detect metabolically active cells, MTT reduction had a higher correlation with sperm concentration and motility parameters (R2= 0.91), than resazurin reduction (R2 = 0.76). We conclude that MTT reduction in stallion spermatozoa follows a species-specific pattern due to a high dependence on oxidative phosphorylation and a degree of NOX activity. As such, MTT reduction is a useful diagnostic tool to assess extra-mitochondrial redox activity, and therefore, the functional qualities of stallion spermatozoa.
    MeSH term(s) Animals ; Formazans ; Horses ; Male ; Mammals ; Mitochondria/metabolism ; Oxidative Phosphorylation ; Sperm Motility ; Spermatozoa/metabolism
    Chemical Substances Formazans
    Language English
    Publishing date 2022-04-22
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2034501-X
    ISSN 1741-7899 ; 1470-1626 ; 1476-3990
    ISSN (online) 1741-7899
    ISSN 1470-1626 ; 1476-3990
    DOI 10.1530/REP-21-0464
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uc I Zs.362: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article: Supplemental Nicotinic Acid Elevates NAD

    Pollard, Charley-Lea / Gibb, Zamira / Clulow, Jennifer / Ruiz, Agustin / Sheridan, Alecia / Bahrami, Mohammad / Swegen, Aleona / Grupen, Christopher G

    Animals : an open access journal from MDPI

    2022  Volume 12, Issue 11

    Abstract: A deficiency in ... ...

    Abstract A deficiency in NAD
    Language English
    Publishing date 2022-05-27
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2606558-7
    ISSN 2076-2615
    ISSN 2076-2615
    DOI 10.3390/ani12111383
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Glycerophospholipids protect stallion spermatozoa from oxidative damage

    Medica, Ashlee J / Aitken, Robert J / Nicolson, Garth L / Sheridan, Alecia R / Swegen, Aleona / De Iuliis, Geoffry N / Gibb, Zamira

    Reproduction & fertility

    2021  Volume 2, Issue 3, Page(s) 199–209

    Abstract: Stallion sperm membranes comprise a high proportion of polyunsaturated fatty acids, making stallion spermatozoa especially vulnerable to peroxidative damage from reactive oxygen species generated as a by-product of cell metabolism. Membrane lipid ... ...

    Abstract Stallion sperm membranes comprise a high proportion of polyunsaturated fatty acids, making stallion spermatozoa especially vulnerable to peroxidative damage from reactive oxygen species generated as a by-product of cell metabolism. Membrane lipid replacement therapy with glycerophospholipid (GPL) mixtures has been shown to reduce oxidative damage
    Lay summary: Sperm collection and storage is an important step in many artificial insemination and
    MeSH term(s) Animals ; Female ; Glycerophospholipids ; Horses ; Humans ; Male ; Oxidative Stress ; Semen ; Sperm Motility ; Spermatozoa
    Chemical Substances Glycerophospholipids
    Language English
    Publishing date 2021-07-21
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 2633-8386
    ISSN (online) 2633-8386
    DOI 10.1530/RAF-21-0028
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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