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  1. Artikel ; Online: Inhibition of degranulation in mast cells attached to a hydrogel through defective microtubule tracts.

    Shiki, Atsushi / Inoh, Yoshikazu / Yokawa, Satoru / Furuno, Tadahide

    Experimental cell research

    2019  Band 381, Heft 2, Seite(n) 248–255

    Abstract: Mast cells (MCs) are important effectors of the immediate allergic response. MCs are distributed throughout various tissues and organs, and adhere to extracellular matrix (ECM) with broad stiffness in the body. Here we compared cellular responses ... ...

    Abstract Mast cells (MCs) are important effectors of the immediate allergic response. MCs are distributed throughout various tissues and organs, and adhere to extracellular matrix (ECM) with broad stiffness in the body. Here we compared cellular responses following antigen stimulation in MCs on glass-base dishes with and without a hydrogel. We found that an antigen-induced increase in intracellular Ca
    Mesh-Begriff(e) Animals ; Calcium/metabolism ; Cell Adhesion/drug effects ; Cell Culture Techniques/instrumentation ; Cell Culture Techniques/methods ; Cell Degranulation/drug effects ; Cell Degranulation/physiology ; Cell Line ; Extracellular Matrix/drug effects ; Extracellular Matrix/metabolism ; Focal Adhesions/drug effects ; Focal Adhesions/metabolism ; Hydrogels/chemistry ; Hydrogels/pharmacology ; Mast Cells/cytology ; Mast Cells/drug effects ; Mast Cells/physiology ; Microtubules/drug effects ; Microtubules/metabolism ; Microtubules/pathology ; Rats ; Signal Transduction/drug effects ; Surface Properties ; Tissue Scaffolds/chemistry
    Chemische Substanzen Hydrogels ; Calcium (SY7Q814VUP)
    Sprache Englisch
    Erscheinungsdatum 2019-05-18
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1493-x
    ISSN 1090-2422 ; 0014-4827
    ISSN (online) 1090-2422
    ISSN 0014-4827
    DOI 10.1016/j.yexcr.2019.05.019
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 563: Hefte anzeigen Standort:
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  2. Artikel ; Online: Promotion of microtubule acetylation plays an important role in degranulation of antigen-activated mast cells.

    Shiki, Atsushi / Inoh, Yoshikazu / Yokawa, Satoru / Furuno, Tadahide

    Inflammation research : official journal of the European Histamine Research Society ... [et al.

    2018  Band 68, Heft 3, Seite(n) 181–184

    Abstract: Objective: The aim of this study was to investigate whether microtubule acetylation is triggered by antigen stimulation and how it affects mast cell degranulation.: Methods: The RBL-2H3 cell line was used as a model for mast cells. Acetylation of α- ... ...

    Abstract Objective: The aim of this study was to investigate whether microtubule acetylation is triggered by antigen stimulation and how it affects mast cell degranulation.
    Methods: The RBL-2H3 cell line was used as a model for mast cells. Acetylation of α-tubulin was analyzed by Western blotting. Intracellular distribution of α-tubulin and acetylated α-tubulin was observed by immunostaining. Degranulation was monitored by measuring the activity of β-hexosaminidase secreted into cell supernatants. Tukey-Kramer test was used to compare differences between groups.
    Results: Microtubule acetylation proceeds globally in mast cell cytoplasm after antigen stimulation in addition to accelerated formation of microtubule-organizing centers. Pretreatment with 5Z-7-oxozeaenol (5 µmol/l), an inhibitor of TGF-β-activated kinase 1, which is a key activator of α-tubulin acetyltransferase 1, did not affect the distribution and acetylation of microtubules in resting cells; however, it significantly suppressed antigen-evoked microtubule acetylation and their reorganization, and subsequent degranulation (95.0 ± 1.2% inhibition, n = 3, P < 0.01).
    Conclusions: These results provided new insight into the post-translational modifications of microtubule to regulate mast cell degranulation.
    Mesh-Begriff(e) Acetylation ; Animals ; Antigens/physiology ; Cell Degranulation ; Cell Line ; Mast Cells/physiology ; Microtubules/physiology ; Rats ; Tubulin/physiology ; beta-N-Acetylhexosaminidases/metabolism
    Chemische Substanzen Antigens ; Tubulin ; beta-N-Acetylhexosaminidases (EC 3.2.1.52)
    Sprache Englisch
    Erscheinungsdatum 2018-11-23
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 1221794-3
    ISSN 1420-908X ; 1023-3830
    ISSN (online) 1420-908X
    ISSN 1023-3830
    DOI 10.1007/s00011-018-1203-2
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 675: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

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