Article: Separation Properties of Plasmid DNA Using a Two-Stage Particle Adsorption-Microfiltration Process.
2023 Volume 13, Issue 2
Abstract: Plasmid DNA is used as a vector for gene therapy and DNA vaccination; therefore, the establishment of a mass production method is required. Membrane filtration is widely employed as a separation method suitable for the mass production of plasmid DNA. ... ...
| Abstract | Plasmid DNA is used as a vector for gene therapy and DNA vaccination; therefore, the establishment of a mass production method is required. Membrane filtration is widely employed as a separation method suitable for the mass production of plasmid DNA. Furthermore, the separation of plasmid DNA using microfiltration and ultrafiltration membranes is being investigated. Because plasmid DNA has a circular structure, it undergoes significant deformation during filtration and easily permeates the membrane, hindering the selection of separation membranes based on molecular weight. In this study, we applied affinity microfiltration to plasmid DNA purification. |
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| Language | English |
| Publishing date | 2023-01-29 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2614641-1 |
| ISSN | 2077-0375 |
| ISSN | 2077-0375 |
| DOI | 10.3390/membranes13020168 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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