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  1. Artikel: Autoimmune Effect of Antibodies against the SARS-CoV-2 Nucleoprotein

    Matyushkina, Daria / Shokina, Varvara / Tikhonova, Polina / Manuvera, Valentin / Shirokov, Dmitry / Kharlampieva, Daria / Lazarev, Vasily / Varizhuk, Anna / Vedekhina, Tatiana / Pavlenko, Alexander / Penkin, Leonid / Arapidi, Georgij / Pavlov, Konstantin / Pushkar, Dmitry / Kolontarev, Konstantin / Rumyantsev, Alexander / Rumyantsev, Sergey / Rychkova, Lyubov / Govorun, Vadim

    Viruses. 2022 May 25, v. 14, no. 6

    2022  

    Abstract: COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an ... ...

    Abstract COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an outpatient basis suffer post-COVID consequences. Partly, it is associated with the autoimmune reactions, so debates about the development of new vaccines and the need for vaccination/revaccination continue. In this study we performed an analysis of the antibody response of patients with COVID-19 to linear and conformational epitopes of viral proteins using ELISA, chip array and western blot with analysis of correlations between antibody titer, disease severity, and complications. We have shown that the presence of IgG antibodies to the nucleoprotein can deteriorate the course of the disease, induce multiple direct COVID-19 symptoms, and contribute to long-term post-covid symptoms. We analyzed the cross reactivity of antibodies to SARS-CoV-2 with own human proteins and showed that antibodies to the nucleocapsid protein can bind to human proteins. In accordance with the possibility of HLA presentation, the main possible targets of the autoantibodies were identified. People with HLA alleles A01:01; A26:01; B39:01; B15:01 are most susceptible to the development of autoimmune processes after COVID-19.
    Schlagwörter COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; Western blotting ; antibody formation ; autoantibodies ; cross reaction ; disease severity ; epitopes ; humans ; nucleocapsid proteins ; nucleoproteins ; people ; vaccination
    Sprache Englisch
    Erscheinungsverlauf 2022-0525
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    ZDB-ID 2516098-9
    ISSN 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061141
    Datenquelle NAL Katalog (AGRICOLA)

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  2. Artikel ; Online: Microarray Profiling of Vaccination-Induced Antibody Responses to SARS-CoV-2 Variants of Interest and Concern.

    Svetlova, Julia / Gustin, Dmitry / Manuvera, Valentin / Shirokov, Dmitriy / Shokina, Varvara / Prusakov, Kirill / Aldarov, Konstantin / Kharlampieva, Daria / Matyushkina, Daria / Bespyatykh, Julia / Varizhuk, Anna / Lazarev, Vassili / Vedekhina, Tatiana

    International journal of molecular sciences

    2022  Band 23, Heft 21

    Abstract: Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction of neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines ... ...

    Abstract Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction of neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines and justify revaccination, rapid evaluation of antibody (Ab) responses to currently circulating SARS-CoV-2 variants of interest (VOI) and concern (VOC) is needed. Here, we developed a multiplex protein microarray-based system for rapid profiling of anti-SARS-CoV-2 Ab levels in human sera. The microarray system was validated using sera samples from SARS-CoV-2-free donors and those diagnosed with COVID-19 based on PCR and enzyme immunoassays. Microarray-based profiling of vaccinated donors revealed a substantial difference in anti-VOC Ab levels elicited by the replication-deficient adenovirus vector-base (Sputnik V) and whole-virion (CoviVac Russia COVID-19) vaccines. Whole-virion vaccine-induced Abs showed minor but statistically significant cross-reactivity with the human blood coagulation factor 1 (fibrinogen) and thrombin. However, their effects on blood clotting were negligible, according to thrombin time tests, providing evidence against the concept of pronounced cross-reactivity-related side effects of the vaccine. Importantly, all samples were collected in the pre-Omicron period but showed noticeable responses to the receptor-binding domain (RBD) of the Omicron spike protein. Thus, using the new express Ab-profiling system, we confirmed the inter-variant cross-reactivity of the anti-SARS-CoV-2 Abs and demonstrated the relative potency of the vaccines against new VOCs.
    Mesh-Begriff(e) Humans ; Antibodies, Neutralizing ; Antibodies, Viral ; Antibody Formation/genetics ; COVID-19/prevention & control ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus/genetics ; Vaccination ; Viral Vaccines/genetics ; Viral Vaccines/pharmacology ; COVID-19 Vaccines/genetics ; COVID-19 Vaccines/pharmacology ; Microarray Analysis
    Chemische Substanzen Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; Viral Vaccines ; COVID-19 Vaccines
    Sprache Englisch
    Erscheinungsdatum 2022-10-30
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms232113220
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Autoimmune Effect of Antibodies against the SARS-CoV-2 Nucleoprotein.

    Matyushkina, Daria / Shokina, Varvara / Tikhonova, Polina / Manuvera, Valentin / Shirokov, Dmitry / Kharlampieva, Daria / Lazarev, Vasily / Varizhuk, Anna / Vedekhina, Tatiana / Pavlenko, Alexander / Penkin, Leonid / Arapidi, Georgij / Pavlov, Konstantin / Pushkar, Dmitry / Kolontarev, Konstantin / Rumyantsev, Alexander / Rumyantsev, Sergey / Rychkova, Lyubov / Govorun, Vadim

    Viruses

    2022  Band 14, Heft 6

    Abstract: COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an ... ...

    Abstract COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, and the severity of the course of the disease itself seems to be decreasing, but even people who have been ill on an outpatient basis suffer post-COVID consequences. Partly, it is associated with the autoimmune reactions, so debates about the development of new vaccines and the need for vaccination/revaccination continue. In this study we performed an analysis of the antibody response of patients with COVID-19 to linear and conformational epitopes of viral proteins using ELISA, chip array and western blot with analysis of correlations between antibody titer, disease severity, and complications. We have shown that the presence of IgG antibodies to the nucleoprotein can deteriorate the course of the disease, induce multiple direct COVID-19 symptoms, and contribute to long-term post-covid symptoms. We analyzed the cross reactivity of antibodies to SARS-CoV-2 with own human proteins and showed that antibodies to the nucleocapsid protein can bind to human proteins. In accordance with the possibility of HLA presentation, the main possible targets of the autoantibodies were identified. People with HLA alleles A01:01; A26:01; B39:01; B15:01 are most susceptible to the development of autoimmune processes after COVID-19.
    Mesh-Begriff(e) Antibodies, Viral ; COVID-19/complications ; Humans ; Nucleoproteins ; SARS-CoV-2 ; Spike Glycoprotein, Coronavirus
    Chemische Substanzen Antibodies, Viral ; Nucleoproteins ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2
    Sprache Englisch
    Erscheinungsdatum 2022-05-25
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2516098-9
    ISSN 1999-4915 ; 1999-4915
    ISSN (online) 1999-4915
    ISSN 1999-4915
    DOI 10.3390/v14061141
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Zusatzmaterialien

    Kategorien

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

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