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  1. Artikel: Survey of Aerobic and Anaerobic Blood Cultures in Free-Ranging Western Ratsnakes (Pantherophis obsoletus)

    Waugh, Lynnette / Cole, Gretchen / D'Agostino, Jennifer / Ramachandran, Akhilesh / Talent, Scott

    Journal of herpetological medicine and surgery. 2017, v. 27, no. 1-2

    2017  

    Abstract: The objective of this study was to survey blood cultures from a healthy population of wild western ratsnakes (Pantherophis obsoletus). Fifteen healthy, adult, free-ranging western ratsnakes were collected from the grounds of the Oklahoma City Zoo in ... ...

    Abstract The objective of this study was to survey blood cultures from a healthy population of wild western ratsnakes (Pantherophis obsoletus). Fifteen healthy, adult, free-ranging western ratsnakes were collected from the grounds of the Oklahoma City Zoo in Oklahoma City, OK. Health assessments included examinations, whole body radiographs, complete blood cell counts, and plasma chemistry analyses. Blood samples were submitted for aerobic and anaerobic blood cultures. Blood cultures from 12 (80%) of 15 snakes were positive for bacteria. The most common bacteria isolated were Morganella morganii, Citrobacter freundii, and Proteus vulgaris. These results may support the possibility of an apparent normal microflora in the blood of healthy western ratsnakes.
    Schlagwörter adults ; bacteria ; blood ; blood cell counts ; blood chemistry ; blood sampling ; Citrobacter freundii ; Elaphe obsoleta ; Morganella morganii ; Proteus vulgaris ; radiography ; snakes ; surveys ; zoos ; Oklahoma
    Sprache Englisch
    Umfang p. 44-47.
    Erscheinungsort Association of Reptilian and Amphibian Veterinarians
    Dokumenttyp Artikel
    ZDB-ID 2634490-7
    ISSN 1529-9651
    ISSN 1529-9651
    Datenquelle NAL Katalog (AGRICOLA)

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  2. Artikel: Evaluation of western fence lizards (Sceloporus occidentalis) and eastern fence lizards (Sceloporus undulatus) as laboratory reptile models for toxicological investigations.

    Talent, Larry G / Dumont, James N / Bantle, John A / Janz, David M / Talent, Scott G

    Environmental toxicology and chemistry

    2002  Band 21, Heft 5, Seite(n) 899–905

    Abstract: A need is recognized for one or more laboratory reptile models for use in ecotoxicological studies and risk assessments. Maintenance of breeding populations of most reptile species under laboratory conditions is not practical because of their size and ... ...

    Abstract A need is recognized for one or more laboratory reptile models for use in ecotoxicological studies and risk assessments. Maintenance of breeding populations of most reptile species under laboratory conditions is not practical because of their size and slow maturation rate. However, a number of species of spiny lizards (Sceloporus sp.) are small, mature quickly, and reproduce under laboratory conditions. We evaluated three populations of western fence lizards (S. occidentalis) and four populations of eastern fence lizards (S. undulatus) for their performance under laboratory conditions. We reared an F1 generation of each population and compared their performance relative to survival, growth, maturation rate, and reproductive output. A population from the San Joaquin Valley (CA. USA) performed especially well under laboratory conditions and is a good candidate for a laboratory model. We also examined the sensitivity of developing fence lizard embryos to an estrogenic chemical to determine if male secondary sex characteristics were affected. Microinjecting eggs with an estrogenic chemical (17alpha-ethinylestradiol) feminized males and prevented development of embryonic secondary sex characteristics. Therefore, embryonic fence lizards may be useful for studying the effects of endocrine-disrupting chemicals.
    Mesh-Begriff(e) Animals ; Animals, Laboratory ; Endocrine System/drug effects ; Environmental Pollutants/adverse effects ; Estradiol/adverse effects ; Estrogens, Non-Steroidal/adverse effects ; Female ; Lizards ; Male ; Models, Theoretical ; Risk Assessment ; Sex Differentiation ; Toxicity Tests
    Chemische Substanzen Environmental Pollutants ; Estrogens, Non-Steroidal ; Estradiol (4TI98Z838E)
    Sprache Englisch
    Erscheinungsdatum 2002-05
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 46234-2
    ISSN 1552-8618 ; 0730-7268
    ISSN (online) 1552-8618
    ISSN 0730-7268
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Genomics accurately predicts antimicrobial resistance in Staphylococcus pseudintermedius collected as part of Vet-LIRN resistance monitoring

    Tyson, Gregory H / Ceric, Olgica / Guag, Jake / Nemser, Sarah / Borenstein, Stacey / Slavic, Durda / Lippert, Sarah / McDowell, Rebecca / Krishnamurthy, Aparna / Korosec, Shannon / Friday, Cheryl / Pople, Neil / Saab, Matthew E / Fairbrother, Julie-Hélène / Janelle, Isabelle / McMillan, Deanna / Bommineni, Yugendar R / Simon, David / Mohan, Shipra /
    Sanchez, Susan / Phillips, Ashley / Bartlett, Paula / Naikare, Hemant / Watson, Cynthia / Sahin, Orhan / Stinman, Chloe / Wang, Leyi / Maddox, Carol / DeShambo, Vanessa / Hendrix, Kenitra / Lubelski, Debra / Burklund, Amy / Lubbers, Brian / Reed, Debbie / Jenkins, Tracie / Erol, Erdal / Patel, Mukeshbhai / Locke, Stephan / Fortner, Jordan / Peak, Laura / Balasuriya, Udeni / Mani, Rinosh / Kettler, Niesa / Olsen, Karen / Zhang, Shuping / Shen, Zhenyu / Landinez, Martha Pulido / Thornton, Jay Kay / Thachil, Anil / Byrd, Melissa / Jacob, Megan / Krogh, Darlene / Webb, Brett / Schaan, Lynn / Patil, Amar / Dasgupta, Sarmila / Mann, Shannon / Goodman, Laura B / Franklin-Guild, Rebecca June / Anderson, Renee R / Mitchell, Patrick K / Cronk, Brittany D / Aprea, Missy / Cui, Jing / Jurkovic, Dominika / Prarat, Melanie / Zhang, Yan / Shiplett, Katherine / Campos, Dubra Diaz / Rubio, Joany Van Balen / Ramanchandran, Akhilesh / Talent, Scott / Tewari, Deepanker / Thirumalapura, Nagaraja / Kelly, Donna / Barnhart, Denise / Hall, Lacey / Rankin, Shelley / Dietrich, Jaclyn / Cole, Stephen / Scaria, Joy / Antony, Linto / Lawhon, Sara D / Wu, Jing / McCoy, Christine / Dietz, Kelly / Wolking, Rebecca / Alexander, Trevor / Burbick, Claire / Reimschuessel, Renate

    Veterinary microbiology. 2021 Mar., v. 254

    2021  

    Abstract: Whole-genome sequencing (WGS) has changed our understanding of bacterial pathogens, aiding outbreak investigations and advancing our knowledge of their genetic features. However, there has been limited use of genomics to understand antimicrobial ... ...

    Abstract Whole-genome sequencing (WGS) has changed our understanding of bacterial pathogens, aiding outbreak investigations and advancing our knowledge of their genetic features. However, there has been limited use of genomics to understand antimicrobial resistance of veterinary pathogens, which would help identify emerging resistance mechanisms and track their spread. The objectives of this study were to evaluate the correlation between resistance genotypes and phenotypes for Staphylococcus pseudintermedius, a major pathogen of companion animals, by comparing broth microdilution antimicrobial susceptibility testing and WGS. From 2017–2019, we conducted antimicrobial susceptibility testing and WGS on S. pseudintermedius isolates collected from dogs in the United States as a part of the Veterinary Laboratory Investigation and Response Network (Vet-LIRN) antimicrobial resistance monitoring program. Across thirteen antimicrobials in nine classes, resistance genotypes correlated with clinical resistance phenotypes 98.4 % of the time among a collection of 592 isolates. Our findings represent isolates from diverse lineages based on phylogenetic analyses, and these strong correlations are comparable to those from studies of several human pathogens such as Staphylococcus aureus and Salmonella enterica. We uncovered some important findings, including that 32.3 % of isolates had the mecA gene, which correlated with oxacillin resistance 97.0 % of the time. We also identified a novel rpoB mutation likely encoding rifampin resistance. These results show the value in using WGS to assess antimicrobial resistance in veterinary pathogens and to reveal putative new mechanisms of resistance.
    Schlagwörter Salmonella enterica ; Staphylococcus aureus ; Staphylococcus pseudintermedius ; antibiotic resistance ; genes ; genomics ; humans ; microbiology ; minimum inhibitory concentration ; mutation ; oxacillin ; phylogeny ; rifampicin
    Sprache Englisch
    Erscheinungsverlauf 2021-03
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    Anmerkung NAL-light
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2021.109006
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 2917: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  4. Artikel ; Online: Genomics accurately predicts antimicrobial resistance in Staphylococcus pseudintermedius collected as part of Vet-LIRN resistance monitoring.

    Tyson, Gregory H / Ceric, Olgica / Guag, Jake / Nemser, Sarah / Borenstein, Stacey / Slavic, Durda / Lippert, Sarah / McDowell, Rebecca / Krishnamurthy, Aparna / Korosec, Shannon / Friday, Cheryl / Pople, Neil / Saab, Matthew E / Fairbrother, Julie-Hélène / Janelle, Isabelle / McMillan, Deanna / Bommineni, Yugendar R / Simon, David / Mohan, Shipra /
    Sanchez, Susan / Phillips, Ashley / Bartlett, Paula / Naikare, Hemant / Watson, Cynthia / Sahin, Orhan / Stinman, Chloe / Wang, Leyi / Maddox, Carol / DeShambo, Vanessa / Hendrix, Kenitra / Lubelski, Debra / Burklund, Amy / Lubbers, Brian / Reed, Debbie / Jenkins, Tracie / Erol, Erdal / Patel, Mukeshbhai / Locke, Stephan / Fortner, Jordan / Peak, Laura / Balasuriya, Udeni / Mani, Rinosh / Kettler, Niesa / Olsen, Karen / Zhang, Shuping / Shen, Zhenyu / Landinez, Martha Pulido / Thornton, Jay Kay / Thachil, Anil / Byrd, Melissa / Jacob, Megan / Krogh, Darlene / Webb, Brett / Schaan, Lynn / Patil, Amar / Dasgupta, Sarmila / Mann, Shannon / Goodman, Laura B / Franklin-Guild, Rebecca June / Anderson, Renee R / Mitchell, Patrick K / Cronk, Brittany D / Aprea, Missy / Cui, Jing / Jurkovic, Dominika / Prarat, Melanie / Zhang, Yan / Shiplett, Katherine / Campos, Dubra Diaz / Rubio, Joany Van Balen / Ramanchandran, Akhilesh / Talent, Scott / Tewari, Deepanker / Thirumalapura, Nagaraja / Kelly, Donna / Barnhart, Denise / Hall, Lacey / Rankin, Shelley / Dietrich, Jaclyn / Cole, Stephen / Scaria, Joy / Antony, Linto / Lawhon, Sara D / Wu, Jing / McCoy, Christine / Dietz, Kelly / Wolking, Rebecca / Alexander, Trevor / Burbick, Claire / Reimschuessel, Renate

    Veterinary microbiology

    2021  Band 254, Seite(n) 109006

    Abstract: Whole-genome sequencing (WGS) has changed our understanding of bacterial pathogens, aiding outbreak investigations and advancing our knowledge of their genetic features. However, there has been limited use of genomics to understand antimicrobial ... ...

    Abstract Whole-genome sequencing (WGS) has changed our understanding of bacterial pathogens, aiding outbreak investigations and advancing our knowledge of their genetic features. However, there has been limited use of genomics to understand antimicrobial resistance of veterinary pathogens, which would help identify emerging resistance mechanisms and track their spread. The objectives of this study were to evaluate the correlation between resistance genotypes and phenotypes for Staphylococcus pseudintermedius, a major pathogen of companion animals, by comparing broth microdilution antimicrobial susceptibility testing and WGS. From 2017-2019, we conducted antimicrobial susceptibility testing and WGS on S. pseudintermedius isolates collected from dogs in the United States as a part of the Veterinary Laboratory Investigation and Response Network (Vet-LIRN) antimicrobial resistance monitoring program. Across thirteen antimicrobials in nine classes, resistance genotypes correlated with clinical resistance phenotypes 98.4 % of the time among a collection of 592 isolates. Our findings represent isolates from diverse lineages based on phylogenetic analyses, and these strong correlations are comparable to those from studies of several human pathogens such as Staphylococcus aureus and Salmonella enterica. We uncovered some important findings, including that 32.3 % of isolates had the mecA gene, which correlated with oxacillin resistance 97.0 % of the time. We also identified a novel rpoB mutation likely encoding rifampin resistance. These results show the value in using WGS to assess antimicrobial resistance in veterinary pathogens and to reveal putative new mechanisms of resistance.
    Mesh-Begriff(e) Animals ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Canada ; Dog Diseases/microbiology ; Dogs/microbiology ; Drug Resistance, Bacterial/genetics ; Epidemiological Monitoring/veterinary ; Genomics/methods ; Genomics/standards ; Genotype ; Microbial Sensitivity Tests ; Phenotype ; Phylogeny ; Reproducibility of Results ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/veterinary ; Staphylococcus/drug effects ; Staphylococcus/genetics ; Staphylococcus/isolation & purification ; United States ; Whole Genome Sequencing
    Chemische Substanzen Anti-Bacterial Agents ; Bacterial Proteins ; MecA protein, Staphylococcus pseudintermedius
    Sprache Englisch
    Erscheinungsdatum 2021-02-04
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 753154-0
    ISSN 1873-2542 ; 0378-1135
    ISSN (online) 1873-2542
    ISSN 0378-1135
    DOI 10.1016/j.vetmic.2021.109006
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

    Volltext online

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    Verfügbar in ZB MED Köln/Königswinter

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