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  1. Artikel ; Online: Production of polyhydroxyalkanoates by a moderately halophilic bacterium of Salinivibrio sp. TGB10.

    Tao, Guan-Bao / Tan, Bi-Wei / Li, Zheng-Jun

    International journal of biological macromolecules

    2021  Band 186, Seite(n) 574–579

    Abstract: A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84  ...

    Abstract A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84 g/L were obtained using glucose as the carbon source after 24 h of cultivation in shake flasks. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized when propionate was provided as secondary carbon source. Salinivibrio sp. TGB10 exhibited favorable tolerance to propionate. The use of 8 g/L propionate and 20 g/L glucose as combinational substrates yielded 1.45 g/L PHBV with a 3-hydroxyvalerate monomer content of 72.02 mol% in flask cultures. In bioreactor study, CDW of 33.45 g/L and PHBV titer of 27.36 g/L were obtained after 108 h of fed-batch cultivation. The results indicated that Salinivibrio sp. TGB10 is a promising halophilic bacterium for the production of PHBV with various polymer compositions.
    Mesh-Begriff(e) Bioreactors ; Fatty Acids, Volatile/metabolism ; Fermentation ; Hydroxybutyrates/metabolism ; Polyesters/metabolism ; Polyhydroxyalkanoates/metabolism ; Substrate Specificity ; Sugars/metabolism ; Vibrionaceae/enzymology ; Vibrionaceae/growth & development ; Water Microbiology
    Chemische Substanzen Fatty Acids, Volatile ; Hydroxybutyrates ; Polyesters ; Polyhydroxyalkanoates ; Sugars ; poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) ; poly-beta-hydroxybutyrate (26063-00-3)
    Sprache Englisch
    Erscheinungsdatum 2021-07-08
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.07.038
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Establishment of a carbon-efficient xylulose cleavage pathway in Escherichia coli to metabolize xylose

    Shi, Li-Long / Zheng, Yuanmin / Tan, Bi-Wei / Li, Zheng-Jun

    Biochemical engineering journal. 2022 Feb., v. 179

    2022  

    Abstract: As the second-largest storage saccharide in the world, xylose is a promising renewable feedstock in the bio-industry. A few microorganisms can metabolize xylose naturally via the xylose isomerase pathway, Weimberg pathway, or Dahms pathway. In this study, ...

    Abstract As the second-largest storage saccharide in the world, xylose is a promising renewable feedstock in the bio-industry. A few microorganisms can metabolize xylose naturally via the xylose isomerase pathway, Weimberg pathway, or Dahms pathway. In this study, we reported the metabolic engineering of Escherichia coli to achieve the carbon-efficient utilization of xylose through the establishment of a rationally designed xylulose cleavage pathway. With the inactivation of ribulose-phosphate 3-epimerase and expression of exogenous phosphoketolase, the engineered E. coli accumulated acetate as a major byproduct using xylose as the sole carbon source. Furtherly, acetate production pathways were successfully blocked to provide sufficient acetyl-CoA precursor for downstream biosynthesis reactions. Finally, we applied the xylulose cleavage pathway to produce poly-3-hydroxybutyrate. Our proposed metabolic engineering strategies illustrate the feasibility of efficient xylose utilization to produce commodity chemicals.
    Schlagwörter Escherichia coli ; acetates ; acetyl coenzyme A ; bioindustry ; biosynthesis ; byproducts ; carbon ; feedstocks ; phosphoketolase ; poly-3-hydroxybutyrate ; xylose ; xylose isomerase ; xylulose
    Sprache Englisch
    Erscheinungsverlauf 2022-02
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 2012139-8
    ISSN 1369-703X
    ISSN 1369-703X
    DOI 10.1016/j.bej.2021.108331
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel: Production of polyhydroxyalkanoates by a moderately halophilic bacterium of Salinivibrio sp. TGB10

    Tao, Guan-Bao / Tan, Bi-Wei / Li, Zheng-Jun

    International journal of biological macromolecules. 2021 Sept. 01, v. 186

    2021  

    Abstract: A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84  ...

    Abstract A moderately halophilic bacterium isolated from the water samples collected from a salt field, Salinivibrio sp. TGB10 was found capable of producing poly-3-hydroxybutytate (PHB) from various sugars. Cell dry weight (CDW) of 8.82 g/L and PHB titer of 6.84 g/L were obtained using glucose as the carbon source after 24 h of cultivation in shake flasks. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) was synthesized when propionate was provided as secondary carbon source. Salinivibrio sp. TGB10 exhibited favorable tolerance to propionate. The use of 8 g/L propionate and 20 g/L glucose as combinational substrates yielded 1.45 g/L PHBV with a 3-hydroxyvalerate monomer content of 72.02 mol% in flask cultures. In bioreactor study, CDW of 33.45 g/L and PHBV titer of 27.36 g/L were obtained after 108 h of fed-batch cultivation. The results indicated that Salinivibrio sp. TGB10 is a promising halophilic bacterium for the production of PHBV with various polymer compositions.
    Schlagwörter Salinivibrio ; bacteria ; bioreactors ; carbon ; glucose ; polyhydroxyalkanoates ; propionic acid
    Sprache Englisch
    Erscheinungsverlauf 2021-0901
    Umfang p. 574-579.
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2021.07.038
    Datenquelle NAL Katalog (AGRICOLA)

    Volltext online

    Zusatzmaterialien

    Kategorien

    Verfügbar in ZB MED Köln/Königswinter

    Zs.B 2374: Hefte anzeigen Standort:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Über subito bestellen

    Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.

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