Artikel ; Online: Establishing a 3D culture system for early organogenesis of monkey embryos ex vivo and single-cell transcriptome analysis of cultured embryos
STAR Protocols, Vol 5, Iss 1, Pp 102835- (2024)
2024
Abstract: Summary: Creating in vitro culture platforms for monkey embryos is crucial for understanding the initial 4 weeks of early primate embryogenesis. Here, we present a protocol to culture cynomolgus monkey embryos in vitro for 25 days post-fertilization and ... ...
Abstract | Summary: Creating in vitro culture platforms for monkey embryos is crucial for understanding the initial 4 weeks of early primate embryogenesis. Here, we present a protocol to culture cynomolgus monkey embryos in vitro for 25 days post-fertilization and to delineate the key developmental events of gastrulation and early organogenesis. We describe steps for culturing with a 3D system, immunofluorescence analysis, single-cell RNA sequencing, and bioinformatic analysis.For complete details on the use and execution of this protocol, please refer to Gong et al. (2023).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
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Schlagwörter | Bioinformatics ; Sequence analysis ; Cell Biology ; Cell culture ; Cell isolation ; Single Cell ; Science (General) ; Q1-390 |
Sprache | Englisch |
Erscheinungsdatum | 2024-03-01T00:00:00Z |
Verlag | Elsevier |
Dokumenttyp | Artikel ; Online |
Datenquelle | BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl) |
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