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  1. Artikel ; Online: Comparative analysis of the susceptibility of Aedes aegypti and Japanese Aedes albopictus to all dengue virus serotypes

    Daisuke Kobayashi / Izumi Kai / Astri Nur Faizah / Meng Ling Moi / Shigeru Tajima / Tomohiko Takasaki / Toshinori Sasaki / Haruhiko Isawa

    Tropical Medicine and Health, Vol 51, Iss 1, Pp 1-

    2023  Band 9

    Abstract: Abstract Background Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. ... ...

    Abstract Abstract Background Dengue fever, caused by the dengue virus (DENV), is the most common viral infection transmitted by Aedes mosquitoes (mainly Ae. aegypti and Ae. albopictus) worldwide. Aedes aegypti is not currently established in Japan, and Ae. albopictus is the primary vector mosquito for DENV in the country, but knowledge of its viral susceptibility is limited. Therefore, we aimed to clarify the status of DENV susceptibility by comparing the infection and dissemination dynamics of Japanese Ae. albopictus to all known DENV serotypes with those of Ae. aegypti. Methods After propagation of each DENV serotype in Vero cells, the culture supernatants were mixed with defibrinated rabbit blood and adenosine triphosphate, and the mixture was artificially blood-sucked by two colonies of Ae. albopictus from Japan and one colony of Ae. aegypti from a dengue-endemic country (Vietnam). After 14 days of sucking, the mosquito body was divided into two parts (thorax/abdomen and head/wings/legs) and total RNA was extracted from each sample. DENV RNA was detected in these extracted RNA samples using a quantitative RT-PCR method specific for each DENV serotype, and infection and dissemination rates were analyzed. Results The Japanese Ae. albopictus colonies were susceptible to all DENV serotypes. Its infection and dissemination rates were significantly lower than those of Ae. aegypti. However, the number of DENV RNA copies in Ae. albopictus was almost not significantly different from that in Ae. aegypti. Furthermore, Japanese Ae. albopictus differed widely in their susceptibility to each DENV serotype. Conclusions In Japanese Ae. albopictus, once DENV overcame the midgut infection barrier, the efficiency of subsequent propagation and dissemination of the virus in the mosquito body was comparable to that of Ae. aegypti. Based on the results of this study and previous dengue outbreak trends, Ae. albopictus is predicted to be highly compatible with DENV-1, suggesting that this serotype poses a high risk for future epidemics in ...
    Schlagwörter Dengue ; DENV ; DENV-1 ; Mosquito ; Aedes ; Aedes albopictus ; Arctic medicine. Tropical medicine ; RC955-962
    Thema/Rubrik (Code) 630
    Sprache Englisch
    Erscheinungsdatum 2023-11-01T00:00:00Z
    Verlag BMC
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  2. Artikel ; Online: Acoustic Emission Measurement by Fiber Bragg Grating Glued to Cylindrical Sensor Holder

    Masanori Takuma / Shigeyoshi Hisada / Kenichi Saitoh / Yoshimasa Takahashi / Yuuki Kobayashi / Akira Kadono / Atushi Murata / Satoshi Iwata / Toshinori Sasaki

    Advances in Materials Science and Engineering, Vol

    2014  Band 2014

    Schlagwörter Physics ; QC1-999 ; Science ; Q ; DOAJ:Physics (General) ; DOAJ:Physics and Astronomy ; Materials of engineering and construction. Mechanics of materials ; TA401-492 ; Electrical engineering. Electronics. Nuclear engineering ; TK1-9971 ; Technology ; T ; DOAJ:Materials ; DOAJ:Technology and Engineering
    Sprache Englisch
    Erscheinungsdatum 2014-01-01T00:00:00Z
    Verlag Hindawi Publishing Corporation
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  3. Artikel: Detection of a novel putative phlebovirus and first isolation of Dugbe virus from ticks in Accra, Ghana

    Kobayashi, Daisuke / Alfred Agbekudzi / Esinam Agbosu / Haruhiko Isawa / J.H. Kofi Bonney / Joannitta Joannides / Joseph H.N. Osei / Kyoko Sawabe / Millicent Opoku / Mitsuko Ohashi / Nobuo Ohta / Ryosuke Fujita / Samuel Dadzie / Toshinori Sasaki

    Ticks and tick-borne diseases. 2017 June, v. 8, no. 4

    2017  

    Abstract: Ticks are ectoparasites that transmit various types of human and animal pathogens. In particular, emerging and re-emerging diseases caused by tick-borne viruses are public health concerns around the world. However, in many countries of the sub-Saharan ... ...

    Abstract Ticks are ectoparasites that transmit various types of human and animal pathogens. In particular, emerging and re-emerging diseases caused by tick-borne viruses are public health concerns around the world. However, in many countries of the sub-Saharan African region, epidemiological information on tick-borne viral infections is limited, and their prevalence and distribution remain largely unknown. In this study, we conducted surveillance on ticks to detect medically important tick-borne bunyaviruses in three study sites in and near to Accra, the capital city of Ghana, in 2015. Domestic dogs and cattle were surveyed and were found to be infested with various tick species belonging to the genera Rhipicephalus, Amblyomma and Haemaphysalis. Importantly, we detected a novel putative phlebovirus in Rhipicephalus ticks, and successfully isolated a new strain of Dugbe virus from Am. variegatum ticks. To our knowledge, this is the first report of tick-associated viruses in Ghana other than Crimean-Congo hemorrhagic fever virus.
    Schlagwörter Amblyomma ; animal pathogens ; cattle ; Crimean-Congo hemorrhagic fever virus ; dogs ; Dugbe virus ; emerging diseases ; Haemaphysalis ; monitoring ; Orthobunyavirus ; Phlebovirus ; Rhipicephalus ; tick-borne diseases ; ticks ; viruses ; Ghana
    Sprache Englisch
    Erscheinungsverlauf 2017-06
    Umfang p. 640-645.
    Erscheinungsort Elsevier GmbH
    Dokumenttyp Artikel
    ZDB-ID 2541872-5
    ISSN 1877-9603 ; 1877-959X
    ISSN (online) 1877-9603
    ISSN 1877-959X
    DOI 10.1016/j.ttbdis.2017.04.010
    Datenquelle NAL Katalog (AGRICOLA)

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  4. Artikel ; Online: Blow Flies Were One of the Possible Candidates for Transmission of Highly Pathogenic H5N1 Avian Influenza Virus during the 2004 Outbreaks in Japan

    Kyoko Sawabe / Keita Hoshino / Haruhiko Isawa / Toshinori Sasaki / Kyeong Soon Kim / Toshihiko Hayashi / Yoshio Tsuda / Hiromu Kurahashi / Mutsuo Kobayashi

    Influenza Research and Treatment, Vol

    2011  Band 2011

    Schlagwörter Infectious and parasitic diseases ; RC109-216 ; Internal medicine ; RC31-1245 ; Medicine ; R ; DOAJ:Internal medicine ; DOAJ:Medicine (General) ; DOAJ:Health Sciences
    Sprache Englisch
    Erscheinungsdatum 2011-01-01T00:00:00Z
    Verlag Hindawi Publishing Corporation
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel: Isolation and characterization of Kabuto Mountain virus, a new tick-borne phlebovirus from Haemaphysalis flava ticks in Japan

    Ejiri, Hiroko / Chang-Kweng Lim / Daisuke Kobayashi / Guillermo Posadas-Herrera / Haruhiko Isawa / Itoe Iizuka-Shiota / Ken Maeda / Koki Kaku / Kyoko Sawabe / Madoka Horiya / Masayuki Saijo / Mutsuo Kobayashi / Mutsuyo Takayama-Ito / Ryosuke Fujita / Ryusei Kuwata / Satsuki Kakiuchi / Shigeru Morikawa / Tetsuya Mizutani / Toshihiko Hayashi /
    Toshinori Sasaki / Yukie Katayama / Yukie Yamaguchi

    Virus research. 2018 Jan. 15, v. 244

    2018  

    Abstract: In Japan, indigenous tick-borne phleboviruses (TBPVs) and their associated diseases first became evident in 2013 by reported human cases of severe fever with thrombocytopenia syndrome (SFTS). In this study, we report a novel member of the genus ... ...

    Abstract In Japan, indigenous tick-borne phleboviruses (TBPVs) and their associated diseases first became evident in 2013 by reported human cases of severe fever with thrombocytopenia syndrome (SFTS). In this study, we report a novel member of the genus Phlebovirus designated as Kabuto Mountain virus (KAMV), which was isolated from the ixodid tick Haemaphysalis flava in Hyogo, Japan. A complete viral genome sequencing and phylogenetic analyses showed that KAMV is a novel member of TBPVs, which is closely related to the Uukuniemi and Kaisodi group viruses. However, unlike the Uukuniemi group viruses, the 165-nt intergenic region (IGR) in the KAMV S segment was highly C-rich in the genomic sense and not predicted to form a secondary structure, which are rather similar to those of the Kaisodi group viruses and most mosquito/sandfly-borne phleboviruses. Furthermore, the NSs protein of KAMV was highly divergent from those of other TBPVs. These results provided further insights into the genetic diversity and evolutionary relationships of TBPVs. KAMV could infect and replicate in some rodent and primate cell lines. We evaluated the infectivity and pathogenicity of KAMV in suckling mice, where we obtained a virulent strain after two passages via intracerebral inoculation. This is the first report showing the existence of a previously unrecognized TBPV in Japan, other than the SFTS virus.
    Schlagwörter cell lines ; fever ; genetic variation ; genomics ; Haemaphysalis ; humans ; intergenic DNA ; mice ; pathogenicity ; phylogeny ; sequence analysis ; SFTS virus ; suckling ; thrombocytopenia ; ticks ; virulent strains ; viruses ; Japan
    Sprache Englisch
    Erscheinungsverlauf 2018-0115
    Umfang p. 252-261.
    Erscheinungsort Elsevier B.V.
    Dokumenttyp Artikel
    ZDB-ID 605780-9
    ISSN 1872-7492 ; 0168-1702
    ISSN (online) 1872-7492
    ISSN 0168-1702
    DOI 10.1016/j.virusres.2017.11.030
    Datenquelle NAL Katalog (AGRICOLA)

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