Artikel ; Online: Defining Integrin Tension Required for Chemotaxis of Metastatic Breast Cancer Cells in Confinement.
2023 Band 12, Heft 25, Seite(n) e2202747
Abstract: Cancer metastasis is affected by chemical factors and physical cues. From cell adhesion to migration, mechanical tension applied to integrin expresses on the cell membrane and physical confinement significantly regulates cancer cell behaviors. Despite ... ...
Abstract | Cancer metastasis is affected by chemical factors and physical cues. From cell adhesion to migration, mechanical tension applied to integrin expresses on the cell membrane and physical confinement significantly regulates cancer cell behaviors. Despite the physical interplay between integrins in cells and ligands in the tumor microenvironment, quantitative analysis of integrin tension during cancer cell migration in microconfined spaces remains elusive owing to the limited experimental tools. Herein, a platform termed microconfinement tension gauge tether to monitor spatial integrin tension with single-molecule precision by analyzing the epithelial-growth-factor-induced chemotaxis of metastatic human breast cancer cells in microfluidic channels is developed. The results reveal that the metastatic cancer cells exert the strongest integrin tension in the range of 54-100 pN at the leading edges of cells during chemokinetic migration on a planar surface, while the cells exert the strongest integrin tension exceeding 100 pN at the cell rear when entering microconfinement. Further analysis demonstrates that cells undergo mesenchymal migration under high integrin tension and less confinement, which is converted to amoeboid migration under low integrin tension or high confinement. In summary, the results identify a basic mechanism underlying the mechanical interactions between integrin tension and microenvironment that determines cancer invasion and metastasis. |
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Mesh-Begriff(e) | Humans ; Female ; Integrins/metabolism ; Breast Neoplasms/pathology ; Chemotaxis ; Cell Adhesion ; Cell Movement ; Cell Line, Tumor ; Tumor Microenvironment |
Chemische Substanzen | Integrins |
Sprache | Englisch |
Erscheinungsdatum | 2023-06-07 |
Erscheinungsland | Germany |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2649576-4 |
ISSN | 2192-2659 ; 2192-2640 |
ISSN (online) | 2192-2659 |
ISSN | 2192-2640 |
DOI | 10.1002/adhm.202202747 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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