Article ; Online: Lis1 Has Two Opposing Modes of Regulating Cytoplasmic Dynein.
2017 Volume 170, Issue 6, Page(s) 1197–1208.e12
Abstract: Regulation is central to the functional versatility of cytoplasmic dynein, a motor involved in intracellular transport, cell division, and neurodevelopment. Previous work established that Lis1, a conserved regulator of dynein, binds to its motor domain ... ...
Abstract | Regulation is central to the functional versatility of cytoplasmic dynein, a motor involved in intracellular transport, cell division, and neurodevelopment. Previous work established that Lis1, a conserved regulator of dynein, binds to its motor domain and induces a tight microtubule-binding state in dynein. The work we present here-a combination of biochemistry, single-molecule assays, and cryoelectron microscopy-led to the surprising discovery that Lis1 has two opposing modes of regulating dynein, being capable of inducing both low and high affinity for the microtubule. We show that these opposing modes depend on the stoichiometry of Lis1 binding to dynein and that this stoichiometry is regulated by the nucleotide state of dynein's AAA3 domain. The low-affinity state requires Lis1 to also bind to dynein at a novel conserved site, mutation of which disrupts Lis1's function in vivo. We propose a new model for the regulation of dynein by Lis1. |
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MeSH term(s) | 1-Alkyl-2-acetylglycerophosphocholine Esterase/chemistry ; 1-Alkyl-2-acetylglycerophosphocholine Esterase/metabolism ; Adenosine Triphosphate/metabolism ; Amino Acid Sequence ; Cryoelectron Microscopy ; Dyneins/chemistry ; Dyneins/metabolism ; Humans ; Microtubule-Associated Proteins/chemistry ; Microtubule-Associated Proteins/metabolism ; Models, Molecular ; Molecular Motor Proteins/metabolism ; Protein Domains ; Saccharomyces cerevisiae/metabolism ; Saccharomyces cerevisiae Proteins/chemistry ; Saccharomyces cerevisiae Proteins/metabolism ; Sequence Alignment |
Chemical Substances | KIP2 protein, S cerevisiae ; Microtubule-Associated Proteins ; Molecular Motor Proteins ; Saccharomyces cerevisiae Proteins ; Adenosine Triphosphate (8L70Q75FXE) ; 1-Alkyl-2-acetylglycerophosphocholine Esterase (EC 3.1.1.47) ; PAFAH1B1 protein, S cerevisiae (EC 3.1.1.47) ; PAFAH1B1 protein, human (EC 3.1.1.47) ; Dyneins (EC 3.6.4.2) |
Language | English |
Publishing date | 2017-09-05 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 187009-9 |
ISSN | 1097-4172 ; 0092-8674 |
ISSN (online) | 1097-4172 |
ISSN | 0092-8674 |
DOI | 10.1016/j.cell.2017.08.037 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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