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  1. Artikel ; Online: CD49b identifies functionally and epigenetically distinct subsets of lineage-biased hematopoietic stem cells.

    Somuncular, Ece / Hauenstein, Julia / Khalkar, Prajakta / Johansson, Anne-Sofie / Dumral, Özge / Frengen, Nicolai S / Gustafsson, Charlotte / Mocci, Giuseppe / Su, Tsu-Yi / Brouwer, Hugo / Trautmann, Christine L / Vanlandewijck, Michael / Orkin, Stuart H / Månsson, Robert / Luc, Sidinh

    Stem cell reports

    2022  Band 17, Heft 7, Seite(n) 1546–1560

    Abstract: Hematopoiesis is maintained by functionally diverse lineage-biased hematopoietic stem cells (HSCs). The functional significance of HSC heterogeneity and the regulatory mechanisms underlying lineage bias are not well understood. However, absolute ... ...

    Abstract Hematopoiesis is maintained by functionally diverse lineage-biased hematopoietic stem cells (HSCs). The functional significance of HSC heterogeneity and the regulatory mechanisms underlying lineage bias are not well understood. However, absolute purification of HSC subtypes with a pre-determined behavior remains challenging, highlighting the importance of continued efforts toward prospective isolation of homogeneous HSC subsets. In this study, we demonstrate that CD49b subdivides the most primitive HSC compartment into functionally distinct subtypes: CD49b
    Mesh-Begriff(e) Cell Differentiation/genetics ; Cell Lineage/genetics ; Hematopoiesis/genetics ; Hematopoietic Stem Cells ; Integrin alpha2 ; Multipotent Stem Cells
    Chemische Substanzen Integrin alpha2
    Sprache Englisch
    Erscheinungsdatum 2022-06-16
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2022.05.014
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells.

    Zvick, Joel / Tarnowska-Sengül, Monika / Ghosh, Adhideb / Bundschuh, Nicola / Gjonlleshaj, Pjeter / Hinte, Laura C / Trautmann, Christine L / Noé, Falko / Qabrati, Xhem / Domenig, Seraina A / Kim, Inseon / Hennek, Thomas / von Meyenn, Ferdinand / Bar-Nur, Ori

    Stem cell reports

    2022  Band 17, Heft 9, Seite(n) 1942–1958

    Abstract: Blastocyst complementation denotes a technique that aims to generate organs, tissues, or cell types in animal chimeras via injection of pluripotent stem cells (PSCs) into genetically compromised blastocyst-stage embryos. Here, we report on successful ... ...

    Abstract Blastocyst complementation denotes a technique that aims to generate organs, tissues, or cell types in animal chimeras via injection of pluripotent stem cells (PSCs) into genetically compromised blastocyst-stage embryos. Here, we report on successful complementation of the male germline in adult chimeras following injection of mouse or rat PSCs into mouse blastocysts carrying a mutation in Tsc22d3, an essential gene for spermatozoa production. Injection of mouse PSCs into Tsc22d3-Knockout (KO) blastocysts gave rise to intraspecies chimeras exclusively embodying PSC-derived functional spermatozoa. In addition, injection of rat embryonic stem cells (rESCs) into Tsc22d3-KO embryos produced interspecies mouse-rat chimeras solely harboring rat spermatids and spermatozoa capable of fertilizing oocytes. Furthermore, using single-cell RNA sequencing, we deconstructed rat spermatogenesis occurring in a mouse-rat chimera testis. Collectively, this study details a method for exclusive xenogeneic germ cell production in vivo, with implications that may extend to rat transgenesis, or endangered animal species conservation efforts.
    Mesh-Begriff(e) Animals ; Blastocyst ; Chimera ; Embryonic Stem Cells ; Male ; Mice ; Mice, Knockout ; Pluripotent Stem Cells ; Rats ; Spermatozoa
    Sprache Englisch
    Erscheinungsdatum 2022-08-04
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2720528-9
    ISSN 2213-6711 ; 2213-6711
    ISSN (online) 2213-6711
    ISSN 2213-6711
    DOI 10.1016/j.stemcr.2022.07.005
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Exclusive generation of rat spermatozoa in sterile mice utilizing blastocyst complementation with pluripotent stem cells

    Zvick, Joel / Tarnowska-Sengül, Monika / Ghosh, Adhideb / id_orcid:0 000-0002-5160-4571 / Bundschuh, Nicola / Gjonlleshaj, Pjeter / Hinte, Laura / id_orcid:0 000-0002-4226-0009 / Trautmann, Christine L. / Noé, Falko / Qabrati, Xhem / Domenig, Seraina A. / Kim, Inseon / Hennek, Thomas / von Meyenn, Ferdinand / id_orcid:0 000-0001-9920-3075 / Bar-Nur, Ori / id_orcid:0 000-0002-6466-3124

    Stem Cell Reports, 17 (9)

    2022  

    Abstract: Blastocyst complementation denotes a technique that aims to generate organs, tissues, or cell types in animal chimeras via injection of pluripotent stem cells (PSCs) into genetically compromised blastocyst-stage embryos. Here, we report on successful ... ...

    Abstract Blastocyst complementation denotes a technique that aims to generate organs, tissues, or cell types in animal chimeras via injection of pluripotent stem cells (PSCs) into genetically compromised blastocyst-stage embryos. Here, we report on successful complementation of the male germline in adult chimeras following injection of mouse or rat PSCs into mouse blastocysts carrying a mutation in Tsc22d3, an essential gene for spermatozoa production. Injection of mouse PSCs into Tsc22d3-Knockout (KO) blastocysts gave rise to intraspecies chimeras exclusively embodying PSC-derived functional spermatozoa. In addition, injection of rat embryonic stem cells (rESCs) into Tsc22d3-KO embryos produced interspecies mouse-rat chimeras solely harboring rat spermatids and spermatozoa capable of fertilizing oocytes. Furthermore, using single-cell RNA sequencing, we deconstructed rat spermatogenesis occurring in a mouse-rat chimera testis. Collectively, this study details a method for exclusive xenogeneic germ cell production in vivo, with implications that may extend to rat transgenesis, or endangered animal species conservation efforts.

    ISSN:2213-6711
    Schlagwörter Stem cell biology ; Germ cell production ; pluripotency ; chimerism
    Sprache Englisch
    Erscheinungsdatum 2022-09-13
    Verlag Elsevier
    Erscheinungsland ch
    Dokumenttyp Artikel ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  4. Artikel ; Online: Antigen presentation safeguards the integrity of the hematopoietic stem cell pool.

    Hernández-Malmierca, Pablo / Vonficht, Dominik / Schnell, Alexandra / Uckelmann, Hannah J / Bollhagen, Alina / Mahmoud, Mohamed A A / Landua, Sophie-Luise / van der Salm, Elise / Trautmann, Christine L / Raffel, Simon / Grünschläger, Florian / Lutz, Raphael / Ghosh, Michael / Renders, Simon / Correia, Nádia / Donato, Elisa / Dixon, Karin O / Hirche, Christoph / Andresen, Carolin /
    Robens, Claudia / Werner, Paula S / Boch, Tobias / Eisel, David / Osen, Wolfram / Pilz, Franziska / Przybylla, Adriana / Klein, Corinna / Buchholz, Frank / Milsom, Michael D / Essers, Marieke A G / Eichmüller, Stefan B / Hofmann, Wolf-Karsten / Nowak, Daniel / Hübschmann, Daniel / Hundemer, Michael / Thiede, Christian / Bullinger, Lars / Müller-Tidow, Carsten / Armstrong, Scott A / Trumpp, Andreas / Kuchroo, Vijay K / Haas, Simon

    Cell stem cell

    2022  Band 29, Heft 5, Seite(n) 760–775.e10

    Abstract: Hematopoietic stem and progenitor cells (HSPCs) are responsible for the production of blood and immune cells. Throughout life, HSPCs acquire oncogenic aberrations that can cause hematological cancers. Although molecular programs maintaining stem cell ... ...

    Abstract Hematopoietic stem and progenitor cells (HSPCs) are responsible for the production of blood and immune cells. Throughout life, HSPCs acquire oncogenic aberrations that can cause hematological cancers. Although molecular programs maintaining stem cell integrity have been identified, safety mechanisms eliminating malignant HSPCs from the stem cell pool remain poorly characterized. Here, we show that HSPCs constitutively present antigens via major histocompatibility complex class II. The presentation of immunogenic antigens, as occurring during malignant transformation, triggers bidirectional interactions between HSPCs and antigen-specific CD4
    Mesh-Begriff(e) Antigen Presentation ; Cell Differentiation ; Hematopoietic Stem Cells ; T-Lymphocytes
    Sprache Englisch
    Erscheinungsdatum 2022-04-30
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2375354-7
    ISSN 1875-9777 ; 1934-5909
    ISSN (online) 1875-9777
    ISSN 1934-5909
    DOI 10.1016/j.stem.2022.04.007
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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