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  1. Artikel: A sequential transformation method for validating soybean genome editing by CRISPR/Cas9 system.

    Trinh, Duy Dinh / Le, Ngoc Thu / Bui, Thao Phuong / Le, Thao Nhu Thi / Nguyen, Cuong Xuan / Chu, Ha Hoang / Do, Phat Tien

    Saudi journal of biological sciences

    2022  Band 29, Heft 10, Seite(n) 103420

    Abstract: This study was performed to evaluate the sequential transformation for soybean genome editing using the CRISPR/Cas9 system as well as to show a strategy for examining the activity of CRISPR/Cas9 constructs, especially the designed guide RNAs (gRNAs). The ...

    Abstract This study was performed to evaluate the sequential transformation for soybean genome editing using the CRISPR/Cas9 system as well as to show a strategy for examining the activity of CRISPR/Cas9 constructs, especially the designed guide RNAs (gRNAs). The gRNAs for targeted mutations of an exogenous gene and multiple endogenous genes were constructed and transferred into a stably-overexpressed-Cas9 soybean line using
    Sprache Englisch
    Erscheinungsdatum 2022-08-17
    Erscheinungsland Saudi Arabia
    Dokumenttyp Journal Article
    ZDB-ID 2515206-3
    ISSN 2213-7106 ; 1319-562X
    ISSN (online) 2213-7106
    ISSN 1319-562X
    DOI 10.1016/j.sjbs.2022.103420
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Simultaneously induced mutations in eIF4E genes by CRISPR/Cas9 enhance PVY resistance in tobacco.

    Le, Ngoc Thu / Tran, Huyen Thi / Bui, Thao Phuong / Nguyen, Giang Thu / Van Nguyen, Doai / Ta, Dong Thi / Trinh, Duy Dinh / Molnar, Attila / Pham, Ngoc Bich / Chu, Ha Hoang / Do, Phat Tien

    Scientific reports

    2022  Band 12, Heft 1, Seite(n) 14627

    Abstract: Tobacco is an important commercial crop and a rich source of alkaloids for pharmaceutical and agricultural applications. However, its yield can be reduced by up to 70% due to virus infections, especially by a potyvirus Potato virus Y (PVY). The ... ...

    Abstract Tobacco is an important commercial crop and a rich source of alkaloids for pharmaceutical and agricultural applications. However, its yield can be reduced by up to 70% due to virus infections, especially by a potyvirus Potato virus Y (PVY). The replication of PVY relies on host factors, and eukaryotic translation initiation factor 4Es (eIF4Es) have already been identified as recessive resistance genes against potyviruses in many plant species. To investigate the molecular basis of PVY resistance in the widely cultivated allotetraploid tobacco variety K326, we developed a dual guide RNA CRISPR/Cas9 system for combinatorial gene editing of two clades, eIF4E1 (eIF4E1-S and eIF4E1-T) and eIF4E2 (eIF4E2-S and eIF4E2-T) in the eIF4E gene family comprising six members in tobacco. We screened for CRISPR/Cas9-induced mutations by heteroduplex analysis and Sanger sequencing, and monitored PVY
    Mesh-Begriff(e) CRISPR-Cas Systems ; Mutation ; Plant Diseases ; Potyvirus ; Solanum tuberosum ; Nicotiana
    Sprache Englisch
    Erscheinungsdatum 2022-08-26
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-022-18923-0
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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