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  1. AU="Usatova, G N"
  2. TI=Hematologic autoimmune disorders in the course of COVID 19: a systematic review of reported cases

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  1. Artikel ; Online: [Influence of siRNA complexes on the reproduction of influenza A virus (Orthomyxoviridae:

    Pashkov, E A / Momot, V Y / Pak, A V / Samoilikov, R V / Pashkov, G A / Usatova, G N / Kravtsova, E O / Poddubikov, A V / Nagieva, F G / Sidorov, A V / Pashkov, E P / Svitich, O A / Zverev, V V

    Voprosy virusologii

    2023  Band 68, Heft 2, Seite(n) 95–104

    Abstract: Introduction: Influenza is one of the most pressing global health problems. Despite the wide range of available anti-influenza drugs, the viral drug resistance is an increasing concern and requires the search for new approaches to overcome it. A ... ...

    Abstract Introduction: Influenza is one of the most pressing global health problems. Despite the wide range of available anti-influenza drugs, the viral drug resistance is an increasing concern and requires the search for new approaches to overcome it. A promising solution is the development of drugs with action that is based on the inhibition of the activity of cellular genes through RNA interference.
    Aim: Evaluation in vivo of the preventive potential of miRNAs directed to the cellular genes FLT4, Nup98 and Nup205 against influenza infection.
    Materials and methods: The A/California/7/09 strain of influenza virus (H1N1) and BALB/c mice were used in the study. The administration of siRNA and experimental infection of animals were performed intranasally. The results of the experiment were analyzed using molecular genetic and virological methods.
    Results: The use of siRNA complexes Nup98.1 and Nup205.1 led to a significant decrease in viral reproduction and concentration of viral RNA on the 3rd day after infection. When two siRNA complexes (Nup98.1 and Nup205.1) were administered simultaneously, a significant decrease in viral titer and concentration of viral RNA was also noted compared with the control groups.
    Conclusions: The use of siRNAs in vivo can lead to an antiviral effect when the activity of single or several cellular genes is suppressed. The results indicate that the use of siRNAs targeting the cellular genes whose expression products are involved in viral reproduction is one of the promising methods for the prevention and treatment of not only influenza, but also other respiratory infections.
    Mesh-Begriff(e) Animals ; Mice ; Humans ; Influenza A virus ; RNA, Small Interfering/genetics ; RNA, Small Interfering/pharmacology ; Influenza A Virus, H1N1 Subtype/genetics ; Influenza A Virus, H1N1 Subtype/metabolism ; Cell Line ; Influenza, Human ; Antiviral Agents/pharmacology ; RNA, Viral ; Reproduction ; Orthomyxoviridae Infections/genetics ; Orthomyxoviridae Infections/prevention & control ; Virus Replication
    Chemische Substanzen RNA, Small Interfering ; Antiviral Agents ; RNA, Viral
    Sprache Russisch
    Erscheinungsdatum 2023-05-18
    Erscheinungsland Russia (Federation)
    Dokumenttyp English Abstract ; Journal Article
    ZDB-ID 201241-8
    ISSN 2411-2097 ; 0507-4088
    ISSN (online) 2411-2097
    ISSN 0507-4088
    DOI 10.36233/0507-4088-159
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Identification and localization of differences between Escherichia coli and Salmonella typhimurium genomes by suppressive subtractive hybridization.

    Bogush, M L / Velikodvorskaya, T V / Lebedev, Y B / Nikolaev, L G / Lukyanov, S A / Fradkov, A F / Pliyev, B K / Boichenko, M N / Usatova, G N / Vorobiev, A A / Andersen, G L / Sverdlov, E D

    Molecular & general genetics : MGG

    1999  Band 262, Heft 4-5, Seite(n) 721–729

    Abstract: The availability of bacterial genome sequences raises an important new problem - how can one move from completely sequenced microorganisms as a reference to the hundreds and thousands of other strains or isolates of the same or related species that will ... ...

    Abstract The availability of bacterial genome sequences raises an important new problem - how can one move from completely sequenced microorganisms as a reference to the hundreds and thousands of other strains or isolates of the same or related species that will not be sequenced in the near future? An efficient way to approach this task is the comparison of genomes by subtractive hybridization. Recently we developed a sensitive and reproducible subtraction procedure for comparison of bacterial genomes, based on the method of suppression subtractive hybridization (SSH). In this work we demonstrate the applicability of subtractive hybridization to the comparison of the related but markedly divergent bacterial species Escherichia coli and Salmonella typhimurium. Clone libraries representing sequence differences were obtained and, in the case of completely sequenced E. coli genome, the differences were directly placed in the genome map. About 60% of the differential clones identified by SSH were present in one of the genomes under comparison and absent from the other. Additional differences in most cases represent sequences that have diverged considerably in the course of evolution. Such an approach to comparative bacterial genomics can be applied both to studies of interspecies evolution - to elucidate the "strategies" that enable different genomes to fit their ecological niches - and to development of diagnostic probes for the rapid identification of pathogenic bacterial species.
    Mesh-Begriff(e) Base Sequence ; DNA Primers ; Escherichia coli/genetics ; Genome, Bacterial ; Nucleic Acid Hybridization ; Salmonella typhimurium/genetics ; Subtraction Technique
    Chemische Substanzen DNA Primers
    Sprache Englisch
    Erscheinungsdatum 1999-12
    Erscheinungsland Germany
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2169-6
    ISSN 1432-1874 ; 1617-4623 ; 0026-8925
    ISSN (online) 1432-1874 ; 1617-4623
    ISSN 0026-8925
    DOI 10.1007/s004380051134
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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