Artikel ; Online: High-Throughput, Parallel Flow Cytometry Screening of Hundreds of Cell Surface Antigens Using Fluorescent Barcoding.
Methods in molecular biology (Clifton, N.J.)
2022 Band 2543, Seite(n) 99–111
Abstract: Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells ...
Abstract | Multicolor flow cytometry allows for analysis of tens of cellular parameters in millions of cells at a single-cell resolution within minutes. The lack of technologies that would facilitate feasible and relatively cheap profiling of such a number of cells with an antibody-based approach led us to the development of a high-throughput cytometry-based platform for surface profiling. We coupled the fluorescent cell barcoding with preexisting, commercially available screening tools to analyze cell surface fingerprint at a large scale. This powerful approach will help to identify novel biomarkers and druggable targets and facilitate the discovery of new concepts in immunology, oncology, and developmental biology. |
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Mesh-Begriff(e) | Antigens, Surface ; Biomarkers/analysis ; Flow Cytometry ; Fluorescent Dyes ; Research |
Chemische Substanzen | Antigens, Surface ; Biomarkers ; Fluorescent Dyes |
Sprache | Englisch |
Erscheinungsdatum | 2022-09-10 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ISSN | 1940-6029 |
ISSN (online) | 1940-6029 |
DOI | 10.1007/978-1-0716-2553-8_9 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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