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  1. Artikel ; Online: Binding of human complement C1 sterase inhibitor to Leptospira spp.

    Breda, Leandro Carvalho Dantas / Vasconcellos, Silvio Arruda / de Moraes Vasconcelos, Dewton / Isaac, Lourdes

    Immunobiology

    2017  Band 223, Heft 2, Seite(n) 183–190

    Abstract: Leptospirosis is an important zoonosis of global importance caused by bacteria Leptospira spp. Pathogenic Leptospira is resistant to Complement System killing while non-pathogenic Leptospira is rapidly killed by exposure to normal human serum (NHS). ... ...

    Abstract Leptospirosis is an important zoonosis of global importance caused by bacteria Leptospira spp. Pathogenic Leptospira is resistant to Complement System killing while non-pathogenic Leptospira is rapidly killed by exposure to normal human serum (NHS). Pathogenic Leptospira interact with Complement Regulators such as Factor H, C4b binding protein and Vitronectin avoiding Complement activation and killing by Alternative and Classical Pathways. One important regulator is C1-inhibitor (C1INH) that interacts with C1s or MASPs controlling the cleavage of C4 and C2 molecules, thereby inhibiting the activation of the Classical and Lectin Pathways. In this study, we demonstrate that attenuated, saprophytic and pathogenic Leptospira interact with C1INH that maintain its regulatory capacity of interaction with C1s preventing the activation of Complement system. Although the interaction with C1INH is not crucial for pathogenic Leptospira survival, it seems to be important for the survival of attenuated and saprophytic Leptospira in normal human serum.
    Mesh-Begriff(e) Animals ; Complement Activation ; Complement C1/metabolism ; Complement C1 Inhibitor Protein/genetics ; Complement C1 Inhibitor Protein/metabolism ; Complement C4b-Binding Protein/metabolism ; Complement Factor H/metabolism ; Food Chain ; Humans ; Immune Evasion ; Leptospiraceae/immunology ; Leptospiraceae/pathogenicity ; Leptospirosis/immunology ; Vaccines, Attenuated ; Virulence ; Vitronectin/metabolism ; Zoonoses
    Chemische Substanzen CFH protein, human ; Complement C1 ; Complement C1 Inhibitor Protein ; Complement C4b-Binding Protein ; SERPING1 protein, human ; Vaccines, Attenuated ; Vitronectin ; Complement Factor H (80295-65-4)
    Sprache Englisch
    Erscheinungsdatum 2017-10-13
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 563292-4
    ISSN 1878-3279 ; 0171-2985
    ISSN (online) 1878-3279
    ISSN 0171-2985
    DOI 10.1016/j.imbio.2017.10.027
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Cytokine Profile in Early Infection by

    Bavia, Lorena / de Castro, Íris A / Amano, Mariane Tami / da Silva, Ana Maria Gonçalves / Vasconcellos, Silvio Arruda / Isaac, Lourdes

    Journal of immunology research

    2019  Band 2019, Seite(n) 1892508

    Abstract: Leptospirosis is considered a neglected disease with an estimated more than one million cases every year. Since rodents are at the same time the main reservoir and generally asymptomatic ... ...

    Abstract Leptospirosis is considered a neglected disease with an estimated more than one million cases every year. Since rodents are at the same time the main reservoir and generally asymptomatic to
    Mesh-Begriff(e) Animals ; Biomarkers ; Biopsy ; Cytokines/blood ; Cytokines/metabolism ; Disease Models, Animal ; Host-Pathogen Interactions ; Leptospira interrogans ; Leptospirosis/blood ; Leptospirosis/metabolism ; Leptospirosis/microbiology ; Leptospirosis/pathology ; Leukocyte Count ; Liver/metabolism ; Liver/microbiology ; Liver/pathology ; Mice ; Organ Specificity ; Time Factors
    Chemische Substanzen Biomarkers ; Cytokines
    Sprache Englisch
    Erscheinungsdatum 2019-10-07
    Erscheinungsland Egypt
    Dokumenttyp Journal Article
    ZDB-ID 2817541-4
    ISSN 2314-7156 ; 2314-8861
    ISSN (online) 2314-7156
    ISSN 2314-8861
    DOI 10.1155/2019/1892508
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  3. Artikel: Draft Genome Sequence of Brazilian Leptospira interrogans Serovar Pomona Strain GR5, Isolated from Apparently Healthy Gilt.

    Moreno, Luisa Zanolli / Miraglia, Fabiana / de Oliveira, Carolina Helena / Vasconcellos, Silvio Arruda / Heinemann, Marcos Bryan / Moreno, Andrea Micke

    Genome announcements

    2018  Band 6, Heft 23

    Abstract: Leptospira ... ...

    Abstract Leptospira interrogans
    Sprache Englisch
    Erscheinungsdatum 2018-06-07
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2704277-7
    ISSN 2169-8287
    ISSN 2169-8287
    DOI 10.1128/genomeA.00491-18
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  4. Artikel ; Online: Serological survey and DNA screening of Leptospira spp. in free-living adult tufted capuchin monkeys (Cebus apella nigritus) in a forest reserve Southeast São Paulo State, Brazil.

    Girio, Raul José Silva / de Andrade-Cruvinel, Tatiana Morosini / Vasconcellos, Silvio Arruda / Repetti, Cláudia Sampaio Fonseca / Friolani, Milena / Bueno, Patrícia Cincotto Dos Santos / Felix, Marcílio / Teixeira, Daniel De Bortoli

    Journal of medical primatology

    2020  Band 50, Heft 1, Seite(n) 3–8

    Abstract: Background: Leptospirosis is an important anthropozoonosis. The study investigated the presence of anti-Leptospira antibodies and detection of Leptospira spp DNA in the urine as well as the biochemical profile in Neotropical wild primates living in a ... ...

    Abstract Background: Leptospirosis is an important anthropozoonosis. The study investigated the presence of anti-Leptospira antibodies and detection of Leptospira spp DNA in the urine as well as the biochemical profile in Neotropical wild primates living in a forest reserve from Southeast São Paulo State, Brazil.
    Methods: Blood samples were obtained from 50 adult tufted capuchin monkeys (Cebus apella nigritus). Urine samples were obtained only from male primates. The screening for antibodies against Leptospira spp was evaluated by microscopic agglutination test (MAT). Leptospira DNA in the urine was evaluated by polymerase chain reaction (PCR) considering the target gene LipL32. Biochemical profile was evaluated by using a spectrophotometer.
    Results: The MAT results included 39 (78%) serum reactive animals with the proportions of 28/39 males and 11/39 females. The most frequent reactive serogroups were Icterohemorrhagiae, Canicola, and Autumnalis. All urine samples were negative for leptospiral DNA. There were no significant differences between sexes for aspartate aminotransferase (AST) and alkaline phosphatase values, but alanine aminotransferase (ALT), creatinine, glucose, and urea were significantly higher in males.
    Conclusions: Tufted capuchin monkeys were sera reactive against leptospirosis. Prevalence was similar for the 2 sexes. Leptospiral DNA was not detected in the urine of sera reactive primates tested by the MAT method. ALT, creatinine, glucose, and urea values were higher in male animals.
    Mesh-Begriff(e) Animals ; Antibodies, Bacterial/blood ; Brazil/epidemiology ; Cebinae ; DNA, Bacterial/urine ; Kidney/microbiology ; Kidney/pathology ; Leptospira/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Leptospirosis/veterinary ; Liver/microbiology ; Liver/pathology ; Male ; Monkey Diseases/epidemiology ; Monkey Diseases/microbiology
    Chemische Substanzen Antibodies, Bacterial ; DNA, Bacterial
    Sprache Englisch
    Erscheinungsdatum 2020-09-02
    Erscheinungsland Denmark
    Dokumenttyp Journal Article
    ZDB-ID 121206-0
    ISSN 1600-0684 ; 0047-2565
    ISSN (online) 1600-0684
    ISSN 0047-2565
    DOI 10.1111/jmp.12493
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Mammalian cell entry (Mce) protein of Leptospira interrogans binds extracellular matrix components, plasminogen and β2 integrin.

    Cosate, Maria Raquel / Siqueira, Gabriela Hase / de Souza, Gisele Oliveira / Vasconcellos, Silvio Arruda / Nascimento, Ana Lucia T O

    Microbiology and immunology

    2016  Band 60, Heft 9, Seite(n) 586–598

    Abstract: A severe re-emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus Leptospira. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and ... ...

    Abstract A severe re-emergingzoonosis, leptospirosis, is caused by pathogenic spirochetes of the genus Leptospira. Several studies have identified leptospiral surface proteins with the ability to bind ECM and plasma components, which could mediate adhesion and invasion through the hosts. It has been shown that Mce of pathogenic Leptospira spp. is an RGD (Arg-Gly-Asp)-motif-dependent virulence factor, responsible for infection of cells and animals. In the present article, we decided to further study the repertoire of the Mce activities in leptospiral biological properties. We report that the recombinant Mce is a broad-spectrum ECM-binding protein, capable of interacting with laminin, cellular and plasma fibronectin and collagen IV. Dose--r-esponse interaction was observed for all the components, fulfilling ligand--receptor requirements. Mce is a PLG binding protein capable to recruit this component from NHS, generating PLA in the presence of PLG activator. Binding of Mce was also observed with the leukocyte cell receptors αLβ2 [(CD11a/CD18)-LFA-1] and αMβ2 [(CD11b/CD18)-Mac-1], suggesting the involvement of this protein in the host immune response. Indeed, virulent Leptospira L1-130 was capable of binding both integrins, whereas culture-attenuated M-20 strain only bind to αMβ2 [(CD11b/CD18)-Mac-1]. To the best of our knowledge, this is the first work to describe that Mce surface protein could mediate the attachment of Leptospira interrogans to human cell receptors αLβ2(CD11a/CD18) and αMβ2(CD11b/CD18).
    Mesh-Begriff(e) Amino Acid Sequence ; Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; CD18 Antigens/metabolism ; Enzyme Activation ; Extracellular Matrix/metabolism ; Fibrinolysin/metabolism ; Host-Pathogen Interactions ; Humans ; Leptospira interrogans/physiology ; Leptospirosis/metabolism ; Leptospirosis/microbiology ; Ligands ; Lymphocyte Function-Associated Antigen-1/metabolism ; Macrophage-1 Antigen/metabolism ; Open Reading Frames ; Plasminogen/metabolism ; Protein Binding ; Recombinant Proteins/chemistry ; Recombinant Proteins/genetics ; Recombinant Proteins/metabolism
    Chemische Substanzen Bacterial Proteins ; CD18 Antigens ; Ligands ; Lymphocyte Function-Associated Antigen-1 ; Macrophage-1 Antigen ; Recombinant Proteins ; Plasminogen (9001-91-6) ; Fibrinolysin (EC 3.4.21.7)
    Sprache Englisch
    Erscheinungsdatum 2016-09
    Erscheinungsland Australia
    Dokumenttyp Journal Article
    ZDB-ID 224792-6
    ISSN 1348-0421 ; 0385-5600
    ISSN (online) 1348-0421
    ISSN 0385-5600
    DOI 10.1111/1348-0421.12406
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  6. Artikel: ANTIBODIES AGAINST BRUCELLA ABORTUS AND LEPTOSPIRA SPP. IN CAPTIVE MAMMALS IN THE STATES OF PARá AND RIO GRANDE DO NORTE, BRAZIL.

    Minervino, Antonio Humberto Hamad / Soares, Herbert Sousa / Barrêto-Júnior, Raimundo Alves / Neves, Kedson Alessandri Lobo / Morini, Adriana Caroprezo / Ortolani, Enrico Lippi / Vasconcellos, Silvio Arruda / Soares, Rodrigo Martins / Gennari, Solange Maria

    Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians

    2018  Band 49, Heft 2, Seite(n) 355–360

    Abstract: Brazil has a large variety of wild animal species, but limited data are available on the occurrence of Brucella abortus and Leptospira spp. antibodies in these animals. Sera from 141 captive mammals belonging to 11 different species from the Northern and ...

    Abstract Brazil has a large variety of wild animal species, but limited data are available on the occurrence of Brucella abortus and Leptospira spp. antibodies in these animals. Sera from 141 captive mammals belonging to 11 different species from the Northern and Northeastern regions of Brazil were screened. Antibodies against B. abortus and Leptospira spp. (24 live serovars) were investigated using the Rose Bengal plate and microscopic agglutination tests, respectively. Associations between the age, gender, and place of captivity were analyzed using the Pearson chi-square or the Fisher exact test. None of the animals were antibody positive for B. abortus. Among the animals tested, 11 (7.8%) were seropositive for Leptospira spp. These included one red brocket deer ( Mazama americana), two tufted capuchin ( Sapajus apella), seven agoutis ( Dasyprocta aguti), and one lowland paca ( Cuniculus paca). No association was observed between sex, age, and the occurrence of Leptospira spp. antibodies ( P > 0.05). However, an association was observed according to the place of captivity ( P = 0.046). From these 11 positive animals, six (54.5%) reacted to the serovars from the Icterohaemorraghiae serogroup, which is mainly responsible for the clinical cases of human leptospirosis in Brazil. To our knowledge, this is the first report of Leptospira spp. antibodies in M. americana and C. paca.
    Mesh-Begriff(e) Animals ; Animals, Zoo ; Antibodies, Bacterial/blood ; Brazil/epidemiology ; Brucella abortus/isolation & purification ; Brucellosis/epidemiology ; Brucellosis/microbiology ; Brucellosis/veterinary ; Cebinae ; Cuniculidae ; Dasyproctidae ; Deer ; Female ; Leptospira/isolation & purification ; Leptospirosis/epidemiology ; Leptospirosis/microbiology ; Leptospirosis/veterinary ; Male ; Monkey Diseases/epidemiology ; Monkey Diseases/microbiology ; Prevalence ; Rodent Diseases/epidemiology ; Rodent Diseases/microbiology ; Seroepidemiologic Studies ; Serogroup
    Chemische Substanzen Antibodies, Bacterial
    Sprache Englisch
    Erscheinungsdatum 2018-06-12
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2174930-9
    ISSN 1937-2825 ; 1042-7260
    ISSN (online) 1937-2825
    ISSN 1042-7260
    DOI 10.1638/2015-0246.1
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  7. Artikel ; Online: Identification of pathogenic and nonpathogenic Leptospira species of Brazilian isolates by Matrix Assisted Laser Desorption/Ionization and Time Flight mass spectrometry.

    Karcher, Daniel / Grenfell, Rafaella C / Moreno, Andrea Micke / Moreno, Luisa Zanolli / Vasconcellos, Silvio Arruda / Heinemann, Marcos B / Junior, Joao N de Almeida / Juliano, Luiz / Juliano, Maria A

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2018  Band 49, Heft 4, Seite(n) 900–908

    Abstract: Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight ... ...

    Abstract Matrix Assisted Laser Desorption/Ionization and Time of Flight mass spectrometry (MALDI-TOF MS) is a powerful tool for the identification of bacteria through the detection and analysis of their proteins or fragments derived from ribosomes. Slight sequence variations in conserved ribosomal proteins distinguish microorganisms at the subspecies and strain levels. Characterization of Leptospira spp. by 16S RNA sequencing is costly and time-consuming, and recent studies have shown that closely related species (e.g., Leptospira interrogans and Leptospira kirschneri) may not be discriminated using this technology. Herein, we report an in-house Leptospira reference spectra database using Leptospira reference strains that were validated with a collection of well-identified Brazilian isolates kept in the Bacterial Zoonosis Laboratory at the Veterinary Preventive Medicine and Animal Health Department at Sao Paulo University. In addition, L. interrogans and L. kirschneri were differentiated using an in-depth mass spectrometry analysis with ClinProTools™ software. In conclusion, our in-house reference spectra database has the necessary accuracy to differentiate pathogenic and non-pathogenic species and to distinguish L. interrogans and L. kirschneri.
    Mesh-Begriff(e) Bacterial Typing Techniques/methods ; Brazil ; DNA, Bacterial/genetics ; Humans ; Leptospira/chemistry ; Leptospira/classification ; Leptospira/genetics ; Leptospira/isolation & purification ; Leptospirosis/microbiology ; RNA, Ribosomal, 16S/genetics ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Tandem Mass Spectrometry/methods
    Chemische Substanzen DNA, Bacterial ; RNA, Ribosomal, 16S
    Sprache Englisch
    Erscheinungsdatum 2018-04-13
    Erscheinungsland Brazil
    Dokumenttyp Evaluation Studies ; Journal Article
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1517-8382
    ISSN (online) 1678-4405
    ISSN 1517-8382
    DOI 10.1016/j.bjm.2018.03.005
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  8. Artikel: Efficacy of leptospiral commercial vaccines on the protection against an autochtonous strain recovered in Brazil

    Sonada, Rafael Bazaglia / Azevedo, Sérgio Santos de / Soto, Francisco Rafael Martins / Costa, Diego Figueiredo da / Morais, Zenaide Maria de / Souza, Gisele Oliveira de / Gonçales, Amane Paldês / Miraglia, Fabiana / Vasconcellos, Sílvio Arruda

    Sociedade Brasileira de Microbiologia Brazilian Journal of Microbiology. 2017,

    2017  

    Abstract: In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five ... ...

    Abstract In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR. Of the five vaccines tested, only two proved effective. None of the surviving animals was positive by culture; however, one animal was positive by PCR. Three of the five vaccines sold commercially in Brazil for the immunization of swine or bovines failed the test of the efficacy to protect the vaccinated animals following challenge with an autochthonous Leptospira strain, Canicola serovar. The two vaccines provided protection against the renal carrier state in the surviving animals. The criteria used to produce leptospirosis bacterins sold commercially in Brazil must be reviewed. The industry should support researches on leptospiral vaccinology to improve the quality of the present vaccines and discover new immunogenic strains, because it is known that vaccination is one of the most important tools to increase the reproduction rates in livestock.
    Schlagwörter Leptospira ; carrier state ; cattle ; industry ; leptospirosis ; microbiology ; polymerase chain reaction ; reproduction ; serotypes ; swine ; vaccination ; vaccine development ; vaccines ; Brazil
    Sprache Englisch
    Erscheinungsort Elsevier Editora Ltda.
    Dokumenttyp Artikel
    Anmerkung Pre-press version
    ZDB-ID 2042078-X
    ISSN 1517-8382
    ISSN 1517-8382
    DOI 10.1016/j.bjm.2017.06.008
    Datenquelle NAL Katalog (AGRICOLA)

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  9. Artikel ; Online: Thin layer microcolony culture associated with PCR for early identification of Mycobacterium bovis.

    do Rosário, Tatiana Reis / Dib, Cristina Corsi / Roxo, Eliana / Pinheiro, Sônia Regina / Vasconcellos, Silvio Arruda / Benites, Nilson Roberti

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2014  Band 45, Heft 1, Seite(n) 225–230

    Abstract: The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of ... ...

    Abstract The initial growth of mycobacteria from 49 samples of cattle and buffalo organs collected in commercial slaughterhouses was compared between modified Middlebrook 7H11 thin layer microcolony culture and Stonebrink medium used in the isolation of Mycobacterium bovis. Aliquots were decontaminated by Petroff's method, processed and cultured in both media. The identity of the acid-fast bacilli stained by Ziehl-Neelsen was confirmed by PCR. Optical microscopy showed that results of the early observation of Mycobacterium bovis colonies in thin layer culture were similar to those obtained in macroscopic observation of the colonies in Stonebrink medium. However, early observation of the colonies enabled early confirmation by PCR, given the shorter time to the visualization of colonies when thin layer culture was used (between the 12(nd) and 25(th) day of culture).
    Mesh-Begriff(e) Abattoirs ; Animals ; Bacteriological Techniques/methods ; Cattle ; Meat/microbiology ; Microscopy ; Molecular Diagnostic Techniques/methods ; Mycobacterium bovis/isolation & purification ; Polymerase Chain Reaction/methods ; Staining and Labeling ; Time Factors ; Tuberculosis, Bovine/diagnosis
    Sprache Englisch
    Erscheinungsdatum 2014-05-02
    Erscheinungsland Brazil
    Dokumenttyp Comparative Study ; Evaluation Study ; Journal Article
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1517-8382
    ISSN (online) 1678-4405
    ISSN 1517-8382
    DOI 10.1590/S1517-83822014005000038
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Efficacy of leptospiral commercial vaccines on the protection against an autochtonous strain recovered in Brazil.

    Sonada, Rafael Bazaglia / Azevedo, Sérgio Santos de / Soto, Francisco Rafael Martins / Costa, Diego Figueiredo da / Morais, Zenaide Maria de / Souza, Gisele Oliveira de / Gonçales, Amane Paldês / Miraglia, Fabiana / Vasconcellos, Sílvio Arruda

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2017  Band 49, Heft 2, Seite(n) 347–350

    Abstract: In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five ... ...

    Abstract In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR. Of the five vaccines tested, only two proved effective. None of the surviving animals was positive by culture; however, one animal was positive by PCR. Three of the five vaccines sold commercially in Brazil for the immunization of swine or bovines failed the test of the efficacy to protect the vaccinated animals following challenge with an autochthonous Leptospira strain, Canicola serovar. The two vaccines provided protection against the renal carrier state in the surviving animals. The criteria used to produce leptospirosis bacterins sold commercially in Brazil must be reviewed. The industry should support researches on leptospiral vaccinology to improve the quality of the present vaccines and discover new immunogenic strains, because it is known that vaccination is one of the most important tools to increase the reproduction rates in livestock.
    Mesh-Begriff(e) Animals ; Bacterial Vaccines/administration & dosage ; Bacterial Vaccines/immunology ; Brazil ; Cattle ; Cattle Diseases/pathology ; Cattle Diseases/prevention & control ; Kidney/microbiology ; Leptospira/immunology ; Leptospira/isolation & purification ; Leptospirosis/pathology ; Leptospirosis/prevention & control ; Leptospirosis/veterinary ; Survival Analysis ; Swine ; Swine Diseases/pathology ; Swine Diseases/prevention & control ; Treatment Outcome
    Chemische Substanzen Bacterial Vaccines
    Sprache Englisch
    Erscheinungsdatum 2017-10-12
    Erscheinungsland Brazil
    Dokumenttyp Journal Article
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1678-9881 ; 1517-8382
    ISSN (online) 1678-4405 ; 1678-9881
    ISSN 1517-8382
    DOI 10.1016/j.bjm.2017.06.008
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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