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  1. Article: Un cas d'infection pulmonaire à Mycobacterium shimoïdei à Madagascar.

    Auregan, G / Ramaroson, F / Génin, C / Vincent Lévy-Frébault, V

    Bulletin de la Societe de pathologie exotique (1990)

    1997  Volume 90, Issue 2, Page(s) 75–77

    Abstract: In 1980, a 32 years-old Madagascan female developed a pulmonary tuberculosis, bacteriologically confirmed. She cured with right apical cavitary sequellae. In 1989, she presented haemoptysis again. Antituberculous treatment was adopted without ... ...

    Title translation A case of Mycobacterium shimoïdei lung infection in Madagascar.
    Abstract In 1980, a 32 years-old Madagascan female developed a pulmonary tuberculosis, bacteriologically confirmed. She cured with right apical cavitary sequellae. In 1989, she presented haemoptysis again. Antituberculous treatment was adopted without bacteriological confirmation and did not improve clinical symptoms. In 1991 and 1992 cultures from sputa and bronchi aspiration yielded acid-fast bacilli identified as Mycobacterium shimoïdei. M. tuberculosis could not be detected. The patient died during treatment. This case is the fourth one in the literature. Whereas previous cases have been reported in Europe, Australia, Asia, this new case shows M. shimoïdei is also present in Africa.
    MeSH term(s) Adult ; Australia ; Bronchi/microbiology ; Europe ; Fatal Outcome ; Female ; Hemoptysis/diagnosis ; Humans ; Japan ; Madagascar ; Mycobacterium Infections, Nontuberculous/diagnosis ; Nontuberculous Mycobacteria/isolation & purification ; Sputum/microbiology ; Tuberculosis, Pulmonary/diagnosis ; Tuberculosis, Pulmonary/microbiology
    Language French
    Publishing date 1997
    Publishing country France
    Document type Case Reports ; English Abstract ; Journal Article
    ZDB-ID 419158-4
    ISSN 1961-9049 ; 0037-9085
    ISSN (online) 1961-9049
    ISSN 0037-9085
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Pulsed field gel electrophoresis of representatives of Mycobacterium tuberculosis and Mycobacterium bovis BCG strains.

    Varnerot, A / Clément, F / Gheorghiu, M / Vincent-Lévy-Frébault, V

    FEMS microbiology letters

    1992  Volume 77, Issue 1-3, Page(s) 155–160

    Abstract: Using field inversion gel electrophoresis (FIGE), different Mycobacterium tuberculosis strains, such as phage prototypes, exhibit different DNA restriction patterns which are easy to compare. Virulent and avirulent variants of M. tuberculosis H37, as ... ...

    Abstract Using field inversion gel electrophoresis (FIGE), different Mycobacterium tuberculosis strains, such as phage prototypes, exhibit different DNA restriction patterns which are easy to compare. Virulent and avirulent variants of M. tuberculosis H37, as well as daughter strains of M. bovis BCG, display characteristic DNA profiles. BCG strains isolated from suppurative adenitis following vaccination of French patients showed patterns identical to the BCG Pasteur strain used for vaccination. These results demonstrate that FIGE of DNA restriction fragments generated by DraI represents a suitable technique for the analysis of mycobacteria at a genomic level. The DraI profiles allow the differentiation and precise identification of the BCG Pasteur, Glaxo, Russian and Japanese strains.
    MeSH term(s) DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Deoxyribonucleases, Type II Site-Specific ; Electrophoresis, Gel, Pulsed-Field ; Humans ; Mycobacterium bovis/genetics ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Mycobacterium tuberculosis/pathogenicity ; Species Specificity ; Tuberculosis/microbiology ; Virulence/genetics
    Chemical Substances DNA, Bacterial ; Deoxyribonucleases, Type II Site-Specific (EC 3.1.21.4) ; TTTAAA -specific type II deoxyribonucleases (EC 3.1.21.4)
    Language English
    Publishing date 1992-11-01
    Publishing country England
    Document type Comparative Study ; Journal Article
    ZDB-ID 752343-9
    ISSN 1574-6968 ; 0378-1097
    ISSN (online) 1574-6968
    ISSN 0378-1097
    DOI 10.1016/0378-1097(92)90148-h
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  3. Article: Evaluation of nonradioactive DNA probes for identification of mycobacteria.

    Lebrun, L / Espinasse, F / Poveda, J D / Vincent-Levy-Frebault, V

    Journal of clinical microbiology

    1992  Volume 30, Issue 9, Page(s) 2476–2478

    Abstract: Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These ... ...

    Abstract Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and 22 Mycobacterium spp. The specificity was 100% for the four probes. The sensitivity was 100% for the MTB complex and M. gordonae probes and 95.2% for the MAC probe. Five of the nine M. kansasii isolates tested were not detected with the probe.
    MeSH term(s) DNA Probes ; Evaluation Studies as Topic ; False Negative Reactions ; Luminescent Measurements ; Mycobacterium/classification ; Mycobacterium/genetics ; Mycobacterium/isolation & purification ; Sensitivity and Specificity
    Chemical Substances DNA Probes
    Language English
    Publishing date 1992-09
    Publishing country United States
    Document type Comparative Study ; Journal Article
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.30.9.2476-2478.1992
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  4. Article: Restriction fragment length polymorphism analysis using IS6110 as an epidemiological marker in tuberculosis.

    Otal, I / Martín, C / Vincent-Lévy-Frebault, V / Thierry, D / Gicquel, B

    Journal of clinical microbiology

    1991  Volume 29, Issue 6, Page(s) 1252–1254

    Abstract: The mycobacterial insertion sequence IS6110 has been shown to be present in multiple copies in the chromosome of Mycobacterium tuberculosis. IS6110 restriction fragment length polymorphism analysis of strains isolated from patients who developed ... ...

    Abstract The mycobacterial insertion sequence IS6110 has been shown to be present in multiple copies in the chromosome of Mycobacterium tuberculosis. IS6110 restriction fragment length polymorphism analysis of strains isolated from patients who developed tuberculosis showed identical patterns over a 2- to 3-year period. In contrast, a high degree of polymorphism was observed between strains of the M. tuberculosis complex isolated from different patients. This study demonstrates that the presence of IS6110 does not induce in vivo major genomic rearrangements over a 2- to 3-year period and confirms its use as a valuable epidemiological marker in tuberculosis.
    MeSH term(s) Base Sequence ; DNA Transposable Elements ; DNA, Bacterial/genetics ; Genetic Markers ; Humans ; Molecular Sequence Data ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Polymorphism, Restriction Fragment Length ; Tuberculosis/epidemiology ; Tuberculosis/microbiology
    Chemical Substances DNA Transposable Elements ; DNA, Bacterial ; Genetic Markers
    Language English
    Publishing date 1991-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.29.6.1252-1254.1991
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning, sequence determination, and genomic organization of the gene coding for antigen 85-C of M. tuberculosis.

    Content, J / de la Cuvellerie, A / De Wit, L / Vincent-Levy-Frébault, V / Ooms, J / De Bruyn, J

    Infection and immunity

    1991  Volume 59, Issue 9, Page(s) 3205–3212

    Abstract: A gene encoding the 33-kDa secreted protein of Mycobacterium tuberculosis (antigen 85-C) was isolated and sequenced. The corresponding DNA sequence contains a 1,020-bp coding region. The deduced amino acid sequence corresponds to a 340-residue protein ... ...

    Abstract A gene encoding the 33-kDa secreted protein of Mycobacterium tuberculosis (antigen 85-C) was isolated and sequenced. The corresponding DNA sequence contains a 1,020-bp coding region. The deduced amino acid sequence corresponds to a 340-residue protein consisting of a 46-amino-acid signal peptide and a 294-amino-acid mature protein. Comparison with previously described genes for the 30-kDa antigen (the alpha antigen of M. bovis BCG, also called antigen 85-B) and the 32-kDa antigens from M. bovis BCG and M. tuberculosis (antigens 85-A) indicates that the three genes share considerable sequence homology (70.8 to 77.5%) but may also code for distinctive epitopes. Strong differences among the three sequences are clearly visible upstream and downstream from the region coding for the mature proteins. The three genes have been detected in the genome of M. bovis BCG by Southern blot hybridization with three type-specific probes. Furthermore, hybridization of large DNA fragments (100 to 1,000 kbp) from M. tuberculosis separated by pulsed-field gel electrophoresis showed that the three genes coding for the antigen 85 complex are not clustered within the bacterial genome.
    MeSH term(s) Amino Acid Sequence ; Antigens, Bacterial/genetics ; Base Sequence ; Blotting, Southern ; DNA, Bacterial/genetics ; Electrophoresis, Polyacrylamide Gel ; Epitopes/immunology ; Molecular Sequence Data ; Mycobacterium bovis/genetics ; Mycobacterium bovis/immunology ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/immunology ; Nucleic Acid Hybridization ; Polymerase Chain Reaction ; Protein Sorting Signals/genetics ; Sequence Homology, Nucleic Acid
    Chemical Substances Antigens, Bacterial ; DNA, Bacterial ; Epitopes ; Protein Sorting Signals
    Language English
    Publishing date 1991-09
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 218698-6
    ISSN 1098-5522 ; 0019-9567
    ISSN (online) 1098-5522
    ISSN 0019-9567
    DOI 10.1128/iai.59.9.3205-3212.1991
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Characterization of a Mycobacterium tuberculosis insertion sequence, IS6110, and its application in diagnosis.

    Thierry, D / Brisson-Noël, A / Vincent-Lévy-Frébault, V / Nguyen, S / Guesdon, J L / Gicquel, B

    Journal of clinical microbiology

    1990  Volume 28, Issue 12, Page(s) 2668–2673

    Abstract: An insertion sequence-like element, IS6110, was isolated from a Mycobacterium tuberculosis cosmid library as a repetitive sequence. IS6110 shows similarities with elements of the IS3 family. This insertion sequence was found to be specific to ... ...

    Abstract An insertion sequence-like element, IS6110, was isolated from a Mycobacterium tuberculosis cosmid library as a repetitive sequence. IS6110 shows similarities with elements of the IS3 family. This insertion sequence was found to be specific to mycobacteria belonging to the M. tuberculosis complex. For detection and identification of M. tuberculosis bacilli in uncultured specimens, oligonucleotides derived from the IS6110 sequence were used as primers and probes in polymerase chain reaction studies. The results obtained were consistent with results of classical identification procedures, bacteriological data, and clinical criteria.
    MeSH term(s) DNA Transposable Elements ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Humans ; Molecular Probes ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Polymerase Chain Reaction/methods ; Sensitivity and Specificity ; Tuberculosis/diagnosis ; Tuberculosis/microbiology
    Chemical Substances DNA Transposable Elements ; DNA, Bacterial ; Molecular Probes
    Language English
    Publishing date 1990-12
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 390499-4
    ISSN 1098-660X ; 0095-1137
    ISSN (online) 1098-660X
    ISSN 0095-1137
    DOI 10.1128/jcm.28.12.2668-2673.1990
    Database MEDical Literature Analysis and Retrieval System OnLINE

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