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  1. Artikel: [Pay more attention to preoperative management of gynecological cancer patients with medical comorbidities].

    Wen, H W

    Zhonghua fu chan ke za zhi

    2016  Band 51, Heft 11, Seite(n) 801–804

    Sprache Chinesisch
    Erscheinungsdatum 2016--25
    Erscheinungsland China
    Dokumenttyp Journal Article
    ZDB-ID 604570-4
    ISSN 0529-567X
    ISSN 0529-567X
    DOI 10.3760/cma.j.issn.0529-567X.2016.11.001
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus.

    Yin, H Y / Fang, T J / Wen, H W

    Letters in applied microbiology

    2016  Band 63, Heft 1, Seite(n) 16–24

    Abstract: Unlabelled: Staphylococcal enterotoxins (SEs) are the most common cause of food poisoning worldwide and can induce symptoms, such as diarrhoea, vomiting and abdominal cramping. Thus, the aim of this study is to develop a multiplex loop-mediated ... ...

    Abstract Unlabelled: Staphylococcal enterotoxins (SEs) are the most common cause of food poisoning worldwide and can induce symptoms, such as diarrhoea, vomiting and abdominal cramping. Thus, the aim of this study is to develop a multiplex loop-mediated isothermal amplification combined with a lateral flow assay (m-LAMP/LFA) to simultaneously detect the sea and seb genes of Staphylococcus aureus. The amplicons of the sea gene were labelled with digoxigenin (Dig) and biotin while those of seb gene were labelled with fluorescein isothiocyanate (FITC) and biotin. These amplicons were detected using a multiplex LFA with NeutrAvidin-tagged gold nanoparticles as the detection reagent. After optimization, the detection limit of this assay was 10(2)  CFU ml(-1) Staph. aureus, which was one tenth that of a multiplex PCR. This assay did not exhibit any cross-reactivity in detecting other enterotoxic Staph. aureus strains or other food pathogens. After 6 h of enrichment, this developed assay detected 1 CFU ml(-1) of Staph. aureus in milk, apple juice, cheese and rice. The developed m-LAMP/LFA method does not require expensive equipment and can be completely implemented within an 8-h workday. Therefore, this method can provide an effective means of quickly screening staphylococcal enterotoxin A- and/or staphylococcal enterotoxin B-producing Staph. aureus in food samples.
    Significance and impact of the study: Staphylococcus aureus is one of the major foodborne pathogens worldwide, and its staphylococcal enterotoxin A and B are strongly associated with food poisoning. This work developed a multiplex loop-mediated isothermal amplification combined with a lateral flow assay (m-LAMP/LFA) to simultaneously detect the sea and seb genes of Staph. aureus in food samples. The assay has good specificity and sensitivity with ease-of-use features, making it ideal for on-site detection.
    Mesh-Begriff(e) Animals ; Cheese/microbiology ; Enterotoxins/genetics ; Food Microbiology ; Fruit and Vegetable Juices/microbiology ; Limit of Detection ; Milk/microbiology ; Multiplex Polymerase Chain Reaction/methods ; Nucleic Acid Amplification Techniques/methods ; Oryza/microbiology ; Sensitivity and Specificity ; Staphylococcus aureus/genetics ; Staphylococcus aureus/pathogenicity
    Chemische Substanzen Enterotoxins ; enterotoxin A, Staphylococcal (37337-57-8) ; enterotoxin B, staphylococcal (39424-53-8)
    Sprache Englisch
    Erscheinungsdatum 2016-07
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 632584-1
    ISSN 1472-765X ; 0266-8254
    ISSN (online) 1472-765X
    ISSN 0266-8254
    DOI 10.1111/lam.12590
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: [Perioperational management of gynecological cancer patients with severe internal medical complications: a serial of 37 clinical cases].

    Hu, J / Lyu, W Q / Guo, Y L / Wen, H W / Qiao, H / Qu, Y

    Zhonghua fu chan ke za zhi

    2016  Band 51, Heft 11, Seite(n) 805–809

    Abstract: Objective: ...

    Abstract Objective:
    Mesh-Begriff(e) Aged ; Anesthesia/adverse effects ; Anesthesia/mortality ; Comorbidity ; Coronary Disease/epidemiology ; Diabetes Mellitus/epidemiology ; Female ; Genital Neoplasms, Female/epidemiology ; Genital Neoplasms, Female/pathology ; Genital Neoplasms, Female/surgery ; Humans ; Hypertension/epidemiology ; Middle Aged ; Perioperative Care ; Perioperative Period ; Postoperative Complications/epidemiology ; Postoperative Complications/mortality ; Retrospective Studies
    Sprache Chinesisch
    Erscheinungsdatum 2016-11-25
    Erscheinungsland China
    Dokumenttyp Evaluation Studies ; Journal Article
    ZDB-ID 604570-4
    ISSN 0529-567X
    ISSN 0529-567X
    DOI 10.3760/cma.j.issn.0529-567X.2016.11.002
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: The feasibility of QR-code prescription in Taiwan.

    Lin, C-H / Tsai, F-Y / Tsai, W-L / Wen, H-W / Hu, M-L

    Journal of clinical pharmacy and therapeutics

    2012  Band 37, Heft 6, Seite(n) 643–646

    Abstract: What is known and objective: An ideal Health Care Service is a service system that focuses on patients. Patients in Taiwan have the freedom to fill their prescriptions at any pharmacies contracted with National Health Insurance. Each of these pharmacies ...

    Abstract What is known and objective: An ideal Health Care Service is a service system that focuses on patients. Patients in Taiwan have the freedom to fill their prescriptions at any pharmacies contracted with National Health Insurance. Each of these pharmacies uses its own computer system. So far, there are at least ten different systems on the market in Taiwan. To transmit the prescription information from the hospital to the pharmacy accurately and efficiently presents a great issue.
    Methods: This study consisted of two-dimensional applications using a QR-code to capture Patient's identification and prescription information from the hospitals as well as using a webcam to read the QR-code and transfer all data to the pharmacy computer system. Two hospitals and 85 community pharmacies participated in the study.
    Results and discussion: During the trial, all participant pharmacies appraised highly of the accurate transmission of the prescription information. The contents in QR-code prescriptions from Taipei area were picked up efficiently and accurately in pharmacies at Taichung area (middle Taiwan) without software system limit and area limitation. The QR-code device received a patent (No. M376844, March 2010) from Intellectual Property Office Ministry of Economic Affair, China.
    What is new and conclusion: Our trial has proven that QR-code prescription can provide community pharmacists an efficient, accurate and inexpensive device to digitalize the prescription contents. Consequently, pharmacists can offer better quality of pharmacy service to patients.
    Mesh-Begriff(e) Automatic Data Processing ; Clinical Pharmacy Information Systems ; Community Pharmacy Services/organization & administration ; Community Pharmacy Services/standards ; Data Collection ; Drug Prescriptions ; Feasibility Studies ; Humans ; National Health Programs/organization & administration ; Pharmacists/organization & administration ; Pharmacists/standards ; Pharmacy Service, Hospital/organization & administration ; Pharmacy Service, Hospital/standards ; Professional Role ; Quality Assurance, Health Care ; Software ; Taiwan
    Sprache Englisch
    Erscheinungsdatum 2012-12
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639006-7
    ISSN 1365-2710 ; 0269-4727
    ISSN (online) 1365-2710
    ISSN 0269-4727
    DOI 10.1111/j.1365-2710.2012.01358.x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Unequal coupling between locomotor pacemakers of the German cockroach, Blattella germanica (L.).

    Wen, H -W. / Lee, H -J.

    Journal of insect physiology

    2000  Band 46, Heft 1, Seite(n) 89–97

    Abstract: Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degrees C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their ... ...

    Abstract Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degrees C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their subsequent regeneration, we demonstrated that the locomotor circadian pacemaker was located in the optic lobes and that it controlled the locomotor circadian rhythm through neural pathways. From the results that unilaterally optic tract severed males still showed locomotor circadian rhythmicity (93.1%, n=29) without significantly changing the circadian period (tau) or level of motor activity, we concluded that the right and left optic lobes each contain a circadian pacemaker competent to drive the locomotor circadian rhythm. These two pacemakers were strongly coupled since only one rhythm was expressed when the pacemakers were independently exposed to opposite lighting conditions (DD or LL) at the same time. However, an unequal contribution of each pacemaker in determining the overt circadian period was found under constant dim light (10 lux) conditions, revealing a major-minor coupling relationship between these two pacemakers, so that the unilaterally blinded male expressed either a LL-rhythm with a circadian period of 24.27+/-0.21 h (41.7%, n=24) or a DD-rhythm with a circadian period of 23.43+/-0.19 h (58.3%, n=24). However, higher intensity of photic information (200-300 lux) could overpower this relationship and cause the minor pacemaker to lead the rhythm.
    Sprache Englisch
    Erscheinungsdatum 2000-01
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 1879-1611
    ISSN (online) 1879-1611
    DOI 10.1016/s0022-1910(99)00105-5
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Constructing liposomal nanovesicles of ginseng extract against hydrogen peroxide-induced oxidative damage to L929 cells

    Tsai, W.-C. / Li, W.-C. / Yin, H.-Y. / Yu, M.-C. / Wen, H.-W.

    Food chemistry

    2012  Band 132, Heft 2, Seite(n) 744

    Sprache Englisch
    Dokumenttyp Artikel
    ZDB-ID 243123-3
    ISSN 0308-8146
    Datenquelle Current Contents Ernährung, Umwelt, Agrar

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  7. Artikel: [Prognostic factors in patients with stage I epithelial ovarian cancer].

    Wen, H W / Liu, S W

    Zhonghua fu chan ke za zhi

    1994  Band 29, Heft 4, Seite(n) 235–7, 255

    Abstract: From 1962 to 1989, 67 patients with stage I epithelial ovarian cancer were treated in our hospital. They were divided into three groups according to the time period of treatment. Thirteen patients treated in the 1960s were in group 1, 23 in the 1970s in ... ...

    Abstract From 1962 to 1989, 67 patients with stage I epithelial ovarian cancer were treated in our hospital. They were divided into three groups according to the time period of treatment. Thirteen patients treated in the 1960s were in group 1, 23 in the 1970s in group 2, and 31 in 1980s in group 3. There were no other significant differences other than the adjuvant chemotherapy in the three groups. In this study, the 5-year survival rate was 46% in group 1 (1962-1969), 82% in group 2 (1970-1979), and 97% in group 3 (1980-1989). The prognosis was improved significantly from 1960s to 1970s (P < 0.005), as well as from 1970s to 1980s (P < 0.05). Multivariate analysis indicated adjuvant chemotherapy (P < 0.01), stage (P < 0.01), and histological grade (P < 0.05) as having independent prognostic effects. Patients with more than 4 courses of combination chemotherapy, stage Ia, grade I and grade II had a better survival rate. The chemotherapy, complete surgical staging and preservation of fertility in surgical treatment of stage I epithelial ovarian cancer were also discussed.
    Mesh-Begriff(e) Adult ; Aged ; Carcinoma/mortality ; Carcinoma/pathology ; Carcinoma/therapy ; Chemotherapy, Adjuvant ; China/epidemiology ; Female ; Humans ; Middle Aged ; Neoplasm Staging ; Ovarian Neoplasms/mortality ; Ovarian Neoplasms/pathology ; Ovarian Neoplasms/therapy ; Prognosis ; Retrospective Studies ; Survival Rate
    Sprache Chinesisch
    Erscheinungsdatum 1994-04
    Erscheinungsland China
    Dokumenttyp Comparative Study ; English Abstract ; Journal Article
    ZDB-ID 604570-4
    ISSN 0529-567X
    ISSN 0529-567X
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel: [48 years malaria control in Xuchang City].

    Wen, H W / Han, C Y / Han, Q Y

    Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases

    2001  Band 19, Heft 3, Seite(n) 185

    Mesh-Begriff(e) China/epidemiology ; Humans ; Malaria/epidemiology ; Malaria/prevention & control ; Mosquito Control ; Population Surveillance
    Sprache Chinesisch
    Erscheinungsdatum 2001
    Erscheinungsland China
    Dokumenttyp Journal Article
    ZDB-ID 639272-6
    ISSN 1000-7423
    ISSN 1000-7423
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel: Unequal coupling between locomotor pacemakers of the German cockroach, Blattella germanica (L.)

    Wen, H.W / Lee, H.J

    Journal of insect physiology. Jan 2000. v. 46 (1)

    2000  

    Abstract: Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degree C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their ... ...

    Abstract Male adult German cockroaches, Blattella germanica (L.), expressed robust locomotor circadian rhythmicity under 28 degree C and constant darkness (DD) conditions. By surgically severing the connections between the optic lobes and midbrain and their subsequent regeneration, we demonstrated that the locomotor circadian pacemaker was located in the optic lobes and that it controlled the locomotor circadian rhythm through neural pathways. From the results that unilaterally optic tract severed males still showed locomotor circadian rhythmicity (93.1%, n=29) without significantly changing the circadian period (tau) or level of motor activity, we concluded that the right and left optic lobes each contain a circadian pacemaker competent to drive the locomotor circadian rhythm. These two pacemakers were strongly coupled since only one rhythm was expressed when the pacemakers were independently exposed to opposite lighting conditions (DD or LL) at the same time. However, an unequal contribution of each pacemaker in determining the overt circadian period was found under constant dim light (10 lux) conditions, revealing a major-minor coupling relationship between these two pacemakers, so that the unilaterally blinded male expressed either a LL-rhythm with a circadian period of 24.27 +/- 0.21 h (41.7%, n=24) or a DD-rhythm with a circadian period of 23.43 +/- 0.19 h (58.3%, n=24).However, higher intensity of photic information (200-300 lux) could overpower this relationship and cause the minor pacemaker to lead the rhythm.
    Schlagwörter Blattella germanica ; light intensity ; circadian rhythm ; locomotion ; brain ; neurophysiology ; peripheral nerves
    Sprache Englisch
    Erscheinungsverlauf 2000-01
    Umfang p. 89-97.
    Dokumenttyp Artikel
    ISSN 0022-1910
    Datenquelle NAL Katalog (AGRICOLA)

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  10. Artikel: A novel extraction method for peanut allergenic proteins in chocolate and their detection by a liposome-based lateral flow assay

    Wen, H.W / Borejsza-Wysocki, W / DeCory, T.R / Baeumner, A.J / Durst, R.A

    European food research and technology = Zeitschrift fur Lebensmittel-Untersuchung und -Forschung. A. 2005 Aug., v. 221, no. 3-4

    2005  

    Schlagwörter chocolate ; peanuts ; allergens ; plant proteins ; extraction ; liposomes (artificial) ; food contamination ; food analysis ; optimization ; hexane ; saline water ; solvents
    Sprache Englisch
    Erscheinungsverlauf 2005-08
    Umfang p. 564-569.
    Dokumenttyp Artikel
    ZDB-ID 1359456-4
    ISSN 1431-4630 ; 1438-2377
    ISSN 1431-4630 ; 1438-2377
    Datenquelle NAL Katalog (AGRICOLA)

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