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  1. Artikel: Slightly acidic electrolyzed water inhibits inflammation induced by membrane vesicles of

    Shimamura, Yuko / Oura, Yukino / Tsuchiya, Madoka / Yamanashi, Yuka / Ogasawara, Asako / Oishi, Minami / Komuro, Misaki / Sasaki, Kuniaki / Masuda, Shuichi

    Frontiers in microbiology

    2024  Band 14, Seite(n) 1328055

    Abstract: Staphylococcus ... ...

    Abstract Staphylococcus aureus
    Sprache Englisch
    Erscheinungsdatum 2024-01-12
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1328055
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Effects of Growth Stage on the Characterization of Enterotoxin A-Producing Staphylococcus aureus-Derived Membrane Vesicles

    Yamanashi, Yuka / Shimamura, Yuko / Sasahara, Haruka / Komuro, Misaki / Sasaki, Kuniaki / Morimitsu, Yasujiro / Masuda, Shuichi

    Microorganisms. 2022 Mar. 06, v. 10, no. 3

    2022  

    Abstract: Virulence factors, such as staphylococcal enterotoxin A (SEA), are contained within membrane vesicles (MVs) in the cell membrane of Staphylococcus aureus. In this study, the effects of the growth stage on quantitative and qualitative changes in the ... ...

    Abstract Virulence factors, such as staphylococcal enterotoxin A (SEA), are contained within membrane vesicles (MVs) in the cell membrane of Staphylococcus aureus. In this study, the effects of the growth stage on quantitative and qualitative changes in the components contained in the MVs of S. aureus SEA-producing strains were examined. Changes in the expression levels of S. aureus genes were examined at each growth stage; phenol-soluble modulin (PSM) gene reached a maximum after 8 h, and the expression of cell membrane-related genes was decreased after 6 h. Based on these gene expression patterns, MVs were prepared at 6, 17, and 24 h. The particle size of MVs did not change depending on the growth stage. MVs prepared after culture for 17 h maintained their particle size when stored at 23 °C. The amount of SEA in the culture supernatant and MVs were not correlated. Bifunctional autolysin, a protein involved in cell wall biosynthesis/degradation, was increased in MVs at 17 h. The expression pattern of inflammation-related genes in human adult low calcium high temperature (HaCaT) cells induced by MVs was different for each growth stage. The inclusion components of S. aureus-derived MVs are selective, depend on the stage of growth, and may play an important role in toxicity.
    Schlagwörter Staphylococcus aureus ; adults ; biosynthesis ; calcium ; cell membranes ; cell walls ; enterotoxins ; gametolysin ; gene expression ; genes ; humans ; particle size ; toxicity ; virulence
    Sprache Englisch
    Erscheinungsverlauf 2022-0306
    Erscheinungsort Multidisciplinary Digital Publishing Institute
    Dokumenttyp Artikel
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10030574
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel: Effects of Growth Stage on the Characterization of Enterotoxin A-Producing

    Yamanashi, Yuka / Shimamura, Yuko / Sasahara, Haruka / Komuro, Misaki / Sasaki, Kuniaki / Morimitsu, Yasujiro / Masuda, Shuichi

    Microorganisms

    2022  Band 10, Heft 3

    Abstract: Virulence factors, such as staphylococcal enterotoxin A (SEA), are contained within membrane vesicles (MVs) in the cell membrane ... ...

    Abstract Virulence factors, such as staphylococcal enterotoxin A (SEA), are contained within membrane vesicles (MVs) in the cell membrane of
    Sprache Englisch
    Erscheinungsdatum 2022-03-06
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2720891-6
    ISSN 2076-2607
    ISSN 2076-2607
    DOI 10.3390/microorganisms10030574
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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