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  1. Artikel: Melittin promotes dexamethasone in the treatment of adjuvant rheumatoid arthritis in rats.

    Yang, Linfu / He, Xiying / Zhi, Dandan / Xue, Yunfei / Gong, Xueyang / Dong, Kun / Tian, Yakai

    Frontiers in pharmacology

    2024  Band 15, Seite(n) 1338432

    Abstract: Background: ...

    Abstract Background:
    Sprache Englisch
    Erscheinungsdatum 2024-02-13
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2587355-6
    ISSN 1663-9812
    ISSN 1663-9812
    DOI 10.3389/fphar.2024.1338432
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Exploring potential network pharmacology-and molecular docking-based mechanism of melittin in treating rheumatoid arthritis.

    Yang, Linfu / Zhao, Wenzheng / Gong, Xueyang / Yue, Dan / Liu, Yiqiu / Tian, Yakai / Dong, Kun

    Medicine

    2023  Band 102, Heft 32, Seite(n) e34728

    Abstract: Background: Rheumatoid arthritis (RA) is a type of difficult-to-cure arthralgia with a worldwide prevalence. It severely affects people's living standards. For a long time, bee venom has been used to treat RA and has shown good results. Melittin is the ... ...

    Abstract Background: Rheumatoid arthritis (RA) is a type of difficult-to-cure arthralgia with a worldwide prevalence. It severely affects people's living standards. For a long time, bee venom has been used to treat RA and has shown good results. Melittin is the main active component of bee venom used for RA treatment, but the molecular mechanism of melittin in RA treatments remains unclear.
    Methods: Potential melittin and RA targets were obtained from relevant databases, and common targets of melittin and RA were screened. The STRING database was used to build the PPI network and screen the core targets after visualization. The core targets were enriched by Gene Ontology functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway. Finally, the binding of melittin to target proteins was evaluated through simulated molecular docking, which verified the reliability of the prediction results of network pharmacology.
    Results: In total, 138 melittin targets and 5795 RA targets were obtained from relevant databases, and 90 common targets were obtained through intersection. Eighteen core targets, such as STAT3, AKT1, tumor necrosis factor, and JUN, were screened out. Enrichment analysis results suggested that melittin plays an anti-RA role mainly through tumor necrosis factor, interleukin-17, toll-like receptors, and advanced glycation end products-RAGE signaling pathways, and pathogenic bacterial infection. Molecular docking results suggested that melittin has good docking activity with core target proteins.
    Conclusion: RA treatment with melittin is the result of a multi-target and multi-pathway interaction. This study offers a theoretical basis and scientific evidence for further exploring melittin in RA therapy.
    Mesh-Begriff(e) Humans ; Melitten/pharmacology ; Melitten/therapeutic use ; Molecular Docking Simulation ; Network Pharmacology ; Reproducibility of Results ; Bee Venoms ; Tumor Necrosis Factor-alpha ; Arthritis, Rheumatoid/drug therapy ; Drugs, Chinese Herbal ; Medicine, Chinese Traditional
    Chemische Substanzen Melitten (20449-79-0) ; Bee Venoms ; Tumor Necrosis Factor-alpha ; Drugs, Chinese Herbal
    Sprache Englisch
    Erscheinungsdatum 2023-07-29
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 80184-7
    ISSN 1536-5964 ; 0025-7974
    ISSN (online) 1536-5964
    ISSN 0025-7974
    DOI 10.1097/MD.0000000000034728
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Amelioration of melittin on adjuvant-induced rheumatoid arthritis: Integrated transcriptome and metabolome.

    Yang, Linfu / He, Xiying / Xue, Yunfei / Zhi, Dandan / Meng, Qingxin / Zhao, Wenzheng / Gong, Xueyang / Yue, Dan / Dong, Kun / Tian, Yakai

    International journal of biological macromolecules

    2024  Band 270, Heft Pt 1, Seite(n) 132293

    Abstract: Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease lacking a definitive cure. Although conventional treatments such as dexamethasone and methotrexate are prevalent, their usage is constrained by potential adverse effects. Melittin ( ... ...

    Abstract Background: Rheumatoid arthritis (RA) is a chronic autoimmune disease lacking a definitive cure. Although conventional treatments such as dexamethasone and methotrexate are prevalent, their usage is constrained by potential adverse effects. Melittin (MLT) has emerged as a promising natural anti-rheumatic drug; however, studies focusing on the role of MLT in modulating the expression and metabolism of RA-related genes are scarce.
    Method: Arthritis was induced in rats using Complete Freund's Adjuvant (CFA), followed by MLT injections for treatment. Post-treatment, the inflammatory status of each group was assessed, and the mechanistic underpinnings of MLT's ameliorative effects on RA were elucidated through transcriptomic and metabolomic analyses. Additionally, this study conducted qRT-PCR validation of key therapeutic genes and characterized the molecular docking interactions of MLT with key receptor proteins (TNF-α and IL-1β) using the AutoDock Vina software.
    Result: MLT significantly diminished redness and swelling in affected joints, ameliorated inflammatory cell infiltration, and mitigated joint damage. Integration of transcriptomic and metabolomic data revealed that MLT predominantly regulated the transcription levels of pathways and genes related to cytokines and immune responses, and the metabolic biomarkers of Sphingomyelin, fatty acid, and flavonoid. qRT-PCR confirmed MLT's downregulation of inflammation-related genes such as Il6, Jak2, Stat3, and Ptx3. Molecular docking simulations demonstrated the stable binding of MLT to TNF-α and IL-1β.
    Conclusion: MLT demonstrated significant efficacy in alleviating RA. This study provides a comprehensive summary of MLT's impact on gene expression and metabolic processes associated with RA.
    Mesh-Begriff(e) Animals ; Rats ; Transcriptome/drug effects ; Melitten/pharmacology ; Arthritis, Rheumatoid/drug therapy ; Arthritis, Rheumatoid/metabolism ; Arthritis, Rheumatoid/chemically induced ; Metabolome/drug effects ; Arthritis, Experimental/drug therapy ; Arthritis, Experimental/metabolism ; Arthritis, Experimental/chemically induced ; Arthritis, Experimental/genetics ; Molecular Docking Simulation ; Freund's Adjuvant ; Male ; Gene Expression Regulation/drug effects ; Gene Expression Profiling
    Chemische Substanzen Melitten (20449-79-0) ; Freund's Adjuvant (9007-81-2)
    Sprache Englisch
    Erscheinungsdatum 2024-05-10
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 282732-3
    ISSN 1879-0003 ; 0141-8130
    ISSN (online) 1879-0003
    ISSN 0141-8130
    DOI 10.1016/j.ijbiomac.2024.132293
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Buch ; Online: Temporal Data Fusion at the Edge

    Yang, Linfu / Liu, Bin

    2019  

    Abstract: As an enabler technique, data fusion has gained great attention in the context of Internet of things (IoT). In traditional settings, data fusion is done at the cloud server. So the data to be fused should be transferred from the sensor nodes to the cloud ...

    Abstract As an enabler technique, data fusion has gained great attention in the context of Internet of things (IoT). In traditional settings, data fusion is done at the cloud server. So the data to be fused should be transferred from the sensor nodes to the cloud server before data fusion. Such an application mode of data fusion inherits disturbing concerns from the cloud computing framework, e.g., privacy-leaking, large latency between data capture and computation, excessive ingress bandwidth consumption. We take into account how to do temporal data fusion at the edge to bypass the above issues. We present a Gaussian process based temporal data fusion (GPTDF) method targeted for the problem of sequential online prediction at the edge. The GPTDF method fits the edge computing framework and thus inherits desirable properties from edge computing, such as privacy-preserving, low latency between data capture and computation, and tiny bandwidth consumption. Through a real-data experiment using archived traffic datasets from the Caltrans Performance Measurement System (PeMS), we demonstrate that the application of GPTDF can provide more timely and accurate real-time predictions at the network edge.

    Comment: 6 pages, 6 figures
    Schlagwörter Computer Science - Distributed ; Parallel ; and Cluster Computing ; Electrical Engineering and Systems Science - Signal Processing
    Erscheinungsdatum 2019-07-28
    Erscheinungsland us
    Dokumenttyp Buch ; Online
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  5. Artikel ; Online: Co-expression of Erns and E2 genes of classical swine fever virus by replication-defective recombinant adenovirus completely protects pigs against virulent challenge with classical swine fever virus.

    Sun, Yongke / Yang, Yuai / Zheng, Huanli / Xi, Dongmei / Lin, Mingxing / Zhang, Xiaomin / Yang, Linfu / Yan, Yulin / Chu, Xiaohui / Bi, Baoliang

    Research in veterinary science

    2013  Band 94, Heft 2, Seite(n) 354–360

    Abstract: The objective of this study was to construct a recombinant adenovirus for future CSFV vaccines used in the pig industry for the reduction of losses involved in CSF outbreaks. The Erns and E2 genes of classical swine fever virus (CSFV), which encode the ... ...

    Abstract The objective of this study was to construct a recombinant adenovirus for future CSFV vaccines used in the pig industry for the reduction of losses involved in CSF outbreaks. The Erns and E2 genes of classical swine fever virus (CSFV), which encode the two main protective glycoproteins from the "Shimen" strain of CSFV, were combined and inserted into the replication-defective human adenovirus type-5 and named the rAd-Erns-E2. Nine pigs were randomly assigned to three treatment groups (three pigs in each group) including the rAd-Erns-E2, hAd-CMV control and DMEM control. Intramuscular vaccination with 2×10(6) TCID(50) of the rAd-Erns-E2 was administered two times with an interval of 21 days. At 42 days post inoculation, pigs in all groups were challenged with a lethal dose of 1×10(3) TCID(50) CSFV "Shimen" strain. Observation of clinical signs was made and the existence of CSFV RNA was detected. Animals in the hAd-CMV and DMEM groups showed severe clinical CSF symptoms and were euthanized from 7 to 10 days after the challenge. However, no adverse clinical CSF signs were observed in vaccinated pigs after the administration of rAd-Erns-E2 and even after CSFV challenge. Neither CSFV RNA nor pathological changes were detected in the tissues of interest of the above vaccinated pigs. These results implied that the recombination adenovirus carrying the Erns-E2 genes could be used to prevent swine from classical swine fever.
    Mesh-Begriff(e) Adenoviridae ; Animals ; Classical Swine Fever/prevention & control ; Classical swine fever virus/pathogenicity ; Gene Expression Regulation, Viral ; HEK293 Cells ; Humans ; Swine ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Virulence ; Virus Replication/genetics
    Chemische Substanzen Viral Proteins
    Sprache Englisch
    Erscheinungsdatum 2013-04
    Erscheinungsland England
    Dokumenttyp Journal Article ; Randomized Controlled Trial ; Research Support, Non-U.S. Gov't
    ZDB-ID 840961-4
    ISSN 1532-2661 ; 0034-5288
    ISSN (online) 1532-2661
    ISSN 0034-5288
    DOI 10.1016/j.rvsc.2012.09.012
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Co-expression of Erns and E2 genes of classical swine fever virus by replication-defective recombinant adenovirus completely protects pigs against virulent challenge with classical swine fever virus

    Sun, Yongke / Yang, Yuai / Zheng, Huanli / Xi, Dongmei / Lin, Mingxing / Zhang, Xiaomin / Yang, Linfu / Yan, Yulin / Chu, Xiaohui / Bi, Baoliang

    Research in veterinary science

    Band v. 94,, Heft no. 2

    Abstract: The objective of this study was to construct a recombinant adenovirus for future CSFV vaccines used in the pig industry for the reduction of losses involved in CSF outbreaks. The Erns and E2 genes of classical swine fever virus (CSFV), which encode the ... ...

    Abstract The objective of this study was to construct a recombinant adenovirus for future CSFV vaccines used in the pig industry for the reduction of losses involved in CSF outbreaks. The Erns and E2 genes of classical swine fever virus (CSFV), which encode the two main protective glycoproteins from the “Shimen” strain of CSFV, were combined and inserted into the replication-defective human adenovirus type-5 and named the rAd-Erns-E2. Nine pigs were randomly assigned to three treatment groups (three pigs in each group) including the rAd-Erns-E2, hAd-CMV control and DMEM control. Intramuscular vaccination with 2×10⁶ TCID₅₀ of the rAd-Erns-E2 was administered two times with an interval of 21days. At 42days post inoculation, pigs in all groups were challenged with a lethal dose of 1×10³ TCID₅₀ CSFV “Shimen” strain. Observation of clinical signs was made and the existence of CSFV RNA was detected. Animals in the hAd-CMV and DMEM groups showed severe clinical CSF symptoms and were euthanized from 7 to 10days after the challenge. However, no adverse clinical CSF signs were observed in vaccinated pigs after the administration of rAd-Erns-E2 and even after CSFV challenge. Neither CSFV RNA nor pathological changes were detected in the tissues of interest of the above vaccinated pigs. These results implied that the recombination adenovirus carrying the Erns-E2 genes could be used to prevent swine from classical swine fever.
    Schlagwörter glycoproteins ; vaccines ; lethal dose ; RNA ; hog cholera ; genes ; humans ; industry ; vaccination ; swine ; Classical swine fever virus
    Sprache Englisch
    Dokumenttyp Artikel
    ISSN 0034-5288
    Datenquelle AGRIS - International Information System for the Agricultural Sciences and Technology

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