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  1. Artikel ; Online: Effects of lipid composition in cationic liposomes on suppression of mast cell activation.

    Inoh, Yoshikazu / Hirose, Takuya / Yokoi, Asami / Yokawa, Satoru / Furuno, Tadahide

    Chemistry and physics of lipids

    2020  Band 231, Seite(n) 104948

    Abstract: We previously showed that cationic liposomes composed of cholesteryl-3β-carboxyamidoethylene-N-hydroxyethylamine (OH-Chol) and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) inhibited mast cell degranulation mediated by the cross-linking of ... ...

    Abstract We previously showed that cationic liposomes composed of cholesteryl-3β-carboxyamidoethylene-N-hydroxyethylamine (OH-Chol) and 1,2-dioleoyl-sn-glycero-3-phosphatidylethanolamine (DOPE) inhibited mast cell degranulation mediated by the cross-linking of high-affinity IgE receptors (FcεRI). In this study, we prepared three kinds of cationic liposomes composed of OH-Chol and DOPE in different ratios (0.28, 0.60, and 0.86 of OH-Chol in mol ratio, named as L-liposome, M-liposome, and H-liposome, respectively) and investigated their effects on mast cell activation. We found that mast cell degranulation evoked with antigen was inhibited by pretreatment with cationic liposomes in the composite ratio-dependent manner of OH-Chol and that the H-liposome showed the highest inhibitory effect on degranulation among three kinds of liposomes. Store-operated Ca
    Mesh-Begriff(e) Animals ; Calcium/metabolism ; Cations/chemistry ; Cells, Cultured ; Endoplasmic Reticulum/drug effects ; Endoplasmic Reticulum/metabolism ; Lipids/chemistry ; Lipids/pharmacology ; Liposomes/chemistry ; Mast Cells/drug effects ; Mast Cells/metabolism ; Rats
    Chemische Substanzen Cations ; Lipids ; Liposomes ; Calcium (SY7Q814VUP)
    Sprache Englisch
    Erscheinungsdatum 2020-07-25
    Erscheinungsland Ireland
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 213869-4
    ISSN 1873-2941 ; 0009-3084
    ISSN (online) 1873-2941
    ISSN 0009-3084
    DOI 10.1016/j.chemphyslip.2020.104948
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Use of single-strand conformation polymorphism of amplified 16S rDNA for grouping of bacteria isolated from foods.

    Takahashi, Hajime / Kimura, Bon / Tanaka, Yuichiro / Mori, Mayumi / Yokoi, Asami / Fujii, Tateo

    Journal of food protection

    2008  Band 71, Heft 4, Seite(n) 839–844

    Abstract: The grouping method for isolated strains from foods using single-strand conformation polymorphism (SSCP) after PCR amplification of a portion of 16S rDNA was developed. This method was able to group the strains from various food samples based on 16S rDNA ...

    Abstract The grouping method for isolated strains from foods using single-strand conformation polymorphism (SSCP) after PCR amplification of a portion of 16S rDNA was developed. This method was able to group the strains from various food samples based on 16S rDNA sequence. As 97.8% of the isolated strains from various foods were grouped correctly, use of the PCR-SSCP method enables the prompt and labor-saving analysis of microbial population of food-derived bacterial strains. Advantages in speed and accuracy of bacterial population identification by the PCR-SSCP method have practical application for food suppliers and testing laboratories.
    Mesh-Begriff(e) Bacteria/classification ; Bacteria/isolation & purification ; Base Sequence ; DNA, Bacterial/genetics ; DNA, Ribosomal ; Food Contamination/analysis ; Food Microbiology ; Gene Amplification ; Gram-Negative Bacteria/classification ; Gram-Negative Bacteria/isolation & purification ; Gram-Positive Bacteria/classification ; Gram-Positive Bacteria/isolation & purification ; Molecular Sequence Data ; Phylogeny ; Polymerase Chain Reaction/methods ; Polymorphism, Single-Stranded Conformational
    Chemische Substanzen DNA, Bacterial ; DNA, Ribosomal
    Sprache Englisch
    Erscheinungsdatum 2008-03-10
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 243284-5
    ISSN 1944-9097 ; 0362-028X
    ISSN (online) 1944-9097
    ISSN 0362-028X
    DOI 10.4315/0362-028x-71.4.839
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Development of multilocus single strand conformation polymorphism (MLSSCP) analysis of virulence genes of Listeria monocytogenes and comparison with existing DNA typing methods.

    Takahashi, Hajime / Handa-Miya, Satoko / Kimura, Bon / Sato, Miki / Yokoi, Asami / Goto, Seitaro / Watanabe, Itaru / Koda, Takashi / Hisa, Kazuo / Fujii, Tateo

    International journal of food microbiology

    2007  Band 118, Heft 3, Seite(n) 274–284

    Abstract: Development of rapid and simple typing methods is required for analyzing the distribution and contamination routes of food-borne pathogens. We established a simple typing method for Listeria monocytogenes using MLSSCP (Multilocus Single Strand ... ...

    Abstract Development of rapid and simple typing methods is required for analyzing the distribution and contamination routes of food-borne pathogens. We established a simple typing method for Listeria monocytogenes using MLSSCP (Multilocus Single Strand Conformation Polymorphism) analysis. Four virulence genes, hlyA, iap, actA and inlB were amplified by PCR, digested with endonucleases and applied to gels for SSCP. As banding patterns have been shown to reflect even a single nucleotide difference, this method has a potential discriminatory power comparable to that of sequencing analysis. The 64 strains isolated from five meat processing plants were divided into 18 groups by this MLSSCP. Additionally, clustering obtained with this method showed strong correspondence with phylogenetic lineages I and II, and was achieved with much less expenditure in time and cost than is required for other methods, such as MLST. The validity of the MLSSCP lineage classification was confirmed by PFGE, AFLP and ribotyping results. This newly developed MLSSCP method is suitable when obtaining accurate results quickly and simply is crucial.
    Mesh-Begriff(e) Cluster Analysis ; Food Contamination/analysis ; Food Microbiology ; Gene Amplification ; Genotype ; Listeria monocytogenes/classification ; Listeria monocytogenes/pathogenicity ; Meat Products/microbiology ; Phylogeny ; Polymerase Chain Reaction/methods ; Polymorphism, Single-Stranded Conformational ; Sensitivity and Specificity ; Species Specificity ; Virulence Factors/genetics
    Chemische Substanzen Virulence Factors
    Sprache Englisch
    Erscheinungsdatum 2007-09-30
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 87122-9
    ISSN 1879-3460 ; 0168-1605
    ISSN (online) 1879-3460
    ISSN 0168-1605
    DOI 10.1016/j.ijfoodmicro.2007.07.047
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

    Zs.A 4668: Hefte anzeigen Standort:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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