Article ; Online: Resveratrol alleviates intestinal mucosal barrier dysfunction in dextran sulfate sodium-induced colitis mice by enhancing autophagy.
World journal of gastroenterology
2020 Volume 26, Issue 33, Page(s) 4945–4959
Abstract: Background: Intestinal mucosal barrier dysfunction plays an important role in the pathogenesis of ulcerative colitis (UC). Recent studies have revealed that impaired autophagy is associated with intestinal mucosal dysfunction in the mucosa of colitis ... ...
Abstract | Background: Intestinal mucosal barrier dysfunction plays an important role in the pathogenesis of ulcerative colitis (UC). Recent studies have revealed that impaired autophagy is associated with intestinal mucosal dysfunction in the mucosa of colitis mice. Resveratrol exerts anti-inflammatory functions by regulating autophagy. Aim: To investigate the effect and mechanism of resveratrol on protecting the integrity of the intestinal mucosal barrier and anti-inflammation in dextran sulfate sodium (DSS)-induced ulcerative colitis mice. Methods: Male C57BL/6 mice were divided into four groups: negative control group, DSS model group, DSS + resveratrol group, and DSS + 5-aminosalicylic acid group. The severity of colitis was assessed by the disease activity index, serum inflammatory cytokines were detected by enzyme-linked immunosorbent assay. Colon tissues were stained with haematoxylin and eosin, and mucosal damage was evaluated by mean histological score. The expression of occludin and ZO-1 in colon tissue was evaluated using immunohistochemical analysis. In addition, the expression of autophagy-related genes was determined using reverse transcription-polymerase chain reaction and Western-blot, and morphology of autophagy was observed by transmission electron microscopy. Results: The resveratrol treatment group showed a 1.72-fold decrease in disease activity index scores and 1.42, 3.81, and 1.65-fold decrease in the production of the inflammatory cytokine tumor necrosis factor-α, interleukin-6 and interleukin-1β, respectively, in DSS-induced colitis mice compared with DSS group ( Conclusion: Resveratrol treatment decreased the expression of inflammatory factors, increased the expression of tight junction proteins and alleviated UC intestinal mucosal barrier dysfunction; this effect may be achieved by enhancing autophagy in intestinal epithelial cells. |
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MeSH term(s) | Animals ; Autophagy ; Colitis/chemically induced ; Colitis/drug therapy ; Colon ; Dextran Sulfate/toxicity ; Disease Models, Animal ; Intestinal Mucosa ; Male ; Mice ; Mice, Inbred C57BL ; Resveratrol/pharmacology |
Chemical Substances | Dextran Sulfate (9042-14-2) ; Resveratrol (Q369O8926L) |
Language | English |
Publishing date | 2020-09-20 |
Publishing country | United States |
Document type | Journal Article |
ZDB-ID | 2185929-2 |
ISSN | 2219-2840 ; 1007-9327 |
ISSN (online) | 2219-2840 |
ISSN | 1007-9327 |
DOI | 10.3748/wjg.v26.i33.4945 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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