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  1. Artikel: Aluminum oxide, cobalt aluminum oxide, and aluminum-doped zinc oxide nanoparticles as an effective antimicrobial agent against pathogens.

    Omeiri, Mohamad / El Hadidi, Esraa / Awad, Ramadan / Al Boukhari, Jamalat / Yusef, Hoda

    Heliyon

    2024  Band 10, Heft 10, Seite(n) e31462

    Abstract: Since the clock of antimicrobial resistance was set, modern medicine has shed light on a new cornerstone in technology to overcome the worldwide dread of the post-antimicrobial era. Research organizations are exploring the use of nanotechnology to modify ...

    Abstract Since the clock of antimicrobial resistance was set, modern medicine has shed light on a new cornerstone in technology to overcome the worldwide dread of the post-antimicrobial era. Research organizations are exploring the use of nanotechnology to modify metallic crystals from macro to nanoscale size, demonstrating significant interest in the field of antimicrobials. Herein, the antimicrobial activities of aluminum oxide (Al
    Sprache Englisch
    Erscheinungsdatum 2024-05-16
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2835763-2
    ISSN 2405-8440
    ISSN 2405-8440
    DOI 10.1016/j.heliyon.2024.e31462
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel ; Online: Isolation and characterization of Candida tropicalis B: a promising yeast strain for biodegradation of petroleum oil in marine environments.

    Hegazy, Ghada E / Soliman, Nadia A / Farag, Soha / El-Helow, Ehab R / Yusef, Hoda Y / Abdel-Fattah, Yasser R

    Microbial cell factories

    2024  Band 23, Heft 1, Seite(n) 20

    Abstract: The increasing interest in environmental protection laws has compelled companies to regulate the disposal of waste organic materials. Despite efforts to explore alternative energy sources, the world remains heavily dependent on crude petroleum oil and ... ...

    Abstract The increasing interest in environmental protection laws has compelled companies to regulate the disposal of waste organic materials. Despite efforts to explore alternative energy sources, the world remains heavily dependent on crude petroleum oil and its derivatives. The expansion of the petroleum industry has significant implications for human and environmental well-being. Bioremediation, employing living microorganisms, presents a promising approach to mitigate the harmful effects of organic hydrocarbons derived from petroleum. This study aimed to isolate and purify local yeast strains from oil-contaminated marine water samples capable of aerobically degrading crude petroleum oils and utilizing them as sole carbon and energy sources. One yeast strain (isolate B) identified as Candida tropicalis demonstrated high potential for biodegrading petroleum oil in seawater. Physiological characterization revealed the strain's ability to thrive across a wide pH range (4-11) with optimal growth at pH 4, as well as tolerate salt concentrations ranging from 1 to 12%. The presence of glucose and yeast extract in the growth medium significantly enhanced the strain's biomass formation and biodegradation capacity. Scanning electron microscopy indicated that the yeast cell diameter varied based on the medium composition, further emphasizing the importance of organic nitrogenous sources for initial growth. Furthermore, the yeast strain exhibited remarkable capabilities in degrading various aliphatic and aromatic hydrocarbons, with a notable preference for naphthalene and phenol at 500 and 1000 mg/l, naphthalene removal reached 97.4% and 98.6%, and phenol removal reached 79.48% and 52.79%, respectively. Optimization experiments using multi-factorial sequential designs highlighted the influential role of oil concentration on the bioremediation efficiency of Candida tropicalis strain B. Moreover, immobilized yeast cells on thin wood chips demonstrated enhanced crude oil degradation compared to thick wood chips, likely due to increased surface area for cell attachment. These findings contribute to our understanding of the potential of Candida tropicalis for petroleum oil bioremediation in marine environments, paving the way for sustainable approaches to address oil pollution.
    Mesh-Begriff(e) Humans ; Candida tropicalis/metabolism ; Biodegradation, Environmental ; Yeasts/metabolism ; Petroleum/metabolism ; Hydrocarbons/metabolism ; Phenol/metabolism ; Naphthalenes/metabolism
    Chemische Substanzen Petroleum ; Hydrocarbons ; Phenol (339NCG44TV) ; Naphthalenes
    Sprache Englisch
    Erscheinungsdatum 2024-01-13
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2091377-1
    ISSN 1475-2859 ; 1475-2859
    ISSN (online) 1475-2859
    ISSN 1475-2859
    DOI 10.1186/s12934-023-02292-y
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel: Monitoring of Pesticide Residues in Lebanese Vegetables and Agricultural Soils and Their Impact on Soil Microbiological Properties

    Omeiri, Mohamad H. / Khnayzer, Rony S. / Yusef, Hoda H.

    Asian journal of water, environment and pollution

    2022  Band 19, Heft 6, Seite(n) 27

    Sprache Englisch
    Dokumenttyp Artikel
    ZDB-ID 2204521-1
    ISSN 0972-9860
    Datenquelle Current Contents Ernährung, Umwelt, Agrar

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  4. Artikel: Bacillus spp. isolated from soil in Lebanon can simultaneously degrade methomyl in contaminated soils and enhance plant growth

    Omeiri, Mohamad / Khnayzer, Rony / Yusef, Hoda / Tokajian, Sima / Salloum, Tamara / Mokh, Samia

    Biocatalysis and agricultural biotechnology. 2022 Jan., v. 39

    2022  

    Abstract: The extensive use of methomyl as a pesticide contributed to its pervasive existence as a pollutant of soil and water resources. In this study, bacteria were isolated from polluted agricultural soils and identified as Bacillus paramycoides and Bacillus ... ...

    Abstract The extensive use of methomyl as a pesticide contributed to its pervasive existence as a pollutant of soil and water resources. In this study, bacteria were isolated from polluted agricultural soils and identified as Bacillus paramycoides and Bacillus proteolyticus. Their methomyl biodegradation efficiencies were evaluated under various experimental conditions in vitro. Bacterial growth was optimized in terms of methomyl concentrations, resulting in maximum growth at 100 mg/L. A considerable degradation was achieved with methomyl being the sole carbon source. Under these conditions, Bacillus spp. degraded 60.6%–62.9% of methomyl in liquid medium when cultured separately, compared to 74.3% degradation when cultured as a consortium within 48 h. Moreover, methomyl bioremediation of artificially contaminated soil varied between 88.3% and 93.2% depending on the used bacterium and soil sterilization status. Furthermore, cell-free extracts of both isolates were tested to inspect their methomyl-degradation activities. The enzymes implicated in such action were mainly located extracellularly, with a lower quota of enzymes available in the intracellular and periplasmic extract. The tested Bacillus spp. also demonstrated considerable corn growth enhancing traits in terms of germination percentages, shoot and root lengths and weights, which were adversely affected by methomyl application. These results illustrate the beneficial use of Bacillus spp. as promising candidates in the bioremediation of methomyl-contaminated sites in conjunction with their contribution as biofertilizers.
    Schlagwörter Bacillus (bacteria) ; agricultural biotechnology ; bacteria ; bacterial growth ; biocatalysis ; biodegradation ; biofertilizers ; bioremediation ; carbon ; corn ; germination ; liquids ; methomyl ; plant growth ; pollutants ; polluted soils ; soil sterilization ; Lebanon
    Sprache Englisch
    Erscheinungsverlauf 2022-01
    Erscheinungsort Elsevier Ltd
    Dokumenttyp Artikel
    ZDB-ID 2642052-1
    ISSN 1878-8181
    ISSN 1878-8181
    DOI 10.1016/j.bcab.2022.102280
    Datenquelle NAL Katalog (AGRICOLA)

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  5. Artikel ; Online: Effects of Wi-Fi Radiofrequency Radiation on Carbapenem-Resistant Klebsiella pneumoniae.

    Said-Salman, Ilham / Yassine, Wissam / Rammal, Ali / Hneino, Mohammad / Yusef, Hoda / Moustafa, Mohamed

    Bioelectromagnetics

    2021  Band 42, Heft 7, Seite(n) 575–582

    Abstract: The hazardous consequences of electromagnetic field (EMF) exposure represent a public health concern. Common sources of EMF include smartphones and wireless fidelity (Wi-Fi). The aim of our study is to assess whether exposure to Wi-Fi radiofrequency ... ...

    Abstract The hazardous consequences of electromagnetic field (EMF) exposure represent a public health concern. Common sources of EMF include smartphones and wireless fidelity (Wi-Fi). The aim of our study is to assess whether exposure to Wi-Fi radiofrequency radiation influences the pathogenic traits of carbapenem-resistant Klebsiella pneumoniae. The susceptibility to antibiotics was evaluated by the determination of minimum inhibitory concentrations (MIC). In this study, K. pneumoniae showed a non-linear response to treatments with Colistin and Gentamycin following different Wi-Fi exposure periods. Transmission electron microscopy revealed morphological changes in the bacterial cell membrane within 24 h of Wi-Fi exposure. Crystal violet quantification and quantitative real-time polymerase chain reaction showed that the ability to form biofilms was greater in Wi-Fi exposed K. pnemoniae when compared to control. Moreover, higher levels of bcsA, mrkA, and luxS messenger RNAs were observed. Our data suggest that Wi-Fi exposure can influence bacteria in a stressful way, leading to an alteration in their antibiotic susceptibility, morphological changes, and cumulative biofilm formation. © 2021 Bioelectromagnetics Society.
    Mesh-Begriff(e) Carbapenems/pharmacology ; Electromagnetic Fields ; Klebsiella pneumoniae ; Radio Waves/adverse effects
    Chemische Substanzen Carbapenems
    Sprache Englisch
    Erscheinungsdatum 2021-08-01
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 760683-7
    ISSN 1521-186X ; 0197-8462
    ISSN (online) 1521-186X
    ISSN 0197-8462
    DOI 10.1002/bem.22364
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: First Case of Identification of Candida kefyr and Pichia kluyveri in Lebanese Water

    Halawi, Mohammad Hassan / Nasser, Roudaina / Yassine, Wissam / Yusef, Hoda / Borjac, Jamilah / Zeaiter, Zaher

    Water, air, and soil pollution. 2020 Mar., v. 231, no. 3

    2020  

    Abstract: Candida kefyr (Kluyveromyces marxianus) is a clinically important infectious yeast. It is found in nature, and is known to cause diseases in humans. No previous studies known have shown the detection of Candida kefyr in water sources of any country, and ... ...

    Abstract Candida kefyr (Kluyveromyces marxianus) is a clinically important infectious yeast. It is found in nature, and is known to cause diseases in humans. No previous studies known have shown the detection of Candida kefyr in water sources of any country, and no studies have detected it anywhere in Lebanon. This present study is carried out to assess the quality of Lebanese water in terms of yeast pollution. The occurrence of Candida spp. (species) was inspected in 84 water samples that tested positive for containing Candida. Out of all isolates, 12 isolates were of C. kefyr and two were of P. kluyveri. The identification of Candida sp. was carried out by using HiCromeTM M1297A and M1297AR chromogenic media. Isolates identified were confirmed by MALDI-TOF MS and 26S rRNA sequencing. The isolates varied from sensitive to resistant to common antifungal agents, including Itraconazole, clotrimazole, fluconazole, miconazole, and nystatin, via disk diffusion assay. These results are the first to confirm the presence of C. kefyr in Lebanese water supplies and suggest that water consumed in Lebanon may be a potential transmission route for serious Candida-based infections.
    Schlagwörter Candida kefyr ; Kluyveromyces marxianus ; Pichia kluyveri ; clotrimazole ; disk diffusion antimicrobial test ; fluconazole ; humans ; itraconazole ; matrix-assisted laser desorption-ionization mass spectrometry ; miconazole ; nystatin ; pollution ; ribosomal RNA ; sequence analysis ; water supply ; yeasts ; Lebanon
    Sprache Englisch
    Erscheinungsverlauf 2020-03
    Umfang p. 108.
    Erscheinungsort Springer International Publishing
    Dokumenttyp Artikel
    ZDB-ID 120499-3
    ISSN 1573-2932 ; 0049-6979 ; 0043-1168
    ISSN (online) 1573-2932
    ISSN 0049-6979 ; 0043-1168
    DOI 10.1007/s11270-020-4460-y
    Datenquelle NAL Katalog (AGRICOLA)

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  7. Artikel ; Online: Global gene expression analysis of Escherichia coli K-12 DH5α after exposure to 2.4 GHz wireless fidelity radiation.

    Said-Salman, Ilham H / Jebaii, Fatima A / Yusef, Hoda H / Moustafa, Mohamed E

    Scientific reports

    2019  Band 9, Heft 1, Seite(n) 14425

    Abstract: This study investigated the non-thermal effects of Wi-Fi radiofrequency radiation of 2.4 GHz on global gene expression in Escherichia coli K-12 DH5α. High-throughput RNA-sequencing of 2.4 GHz exposed and non-exposed bacteria revealed that 101 genes were ... ...

    Abstract This study investigated the non-thermal effects of Wi-Fi radiofrequency radiation of 2.4 GHz on global gene expression in Escherichia coli K-12 DH5α. High-throughput RNA-sequencing of 2.4 GHz exposed and non-exposed bacteria revealed that 101 genes were differentially expressed (DEGs) at P ≤ 0.05. The up-regulated genes were 52 while the down-regulated ones were 49. QRT-PCR analysis of pgaD, fliC, cheY, malP, malZ, motB, alsC, alsK, appB and appX confirmed the RNA-seq results. About 7% of DEGs are involved in cellular component organization, 6% in response to stress stimulus, 6% in biological regulation, 6% in localization, 5% in locomotion and 3% in cell adhesion. Database for annotation, visualization and integrated discovery (DAVID) functional clustering revealed that DEGs with high enrichment score included genes for localization of cell, locomotion, chemotaxis, response to external stimulus and cell adhesion. Kyoto encyclopedia of genes and genomes (KEGG) pathways analysis showed that the pathways for flagellar assembly, chemotaxis and two-component system were affected. Go enrichment analysis indicated that the up-regulated DEGs are involved in metabolic pathways, transposition, response to stimuli, motility, chemotaxis and cell adhesion. The down-regulated DEGs are associated with metabolic pathways and localization of ions and organic molecules. Therefore, the exposure of E. coli DH5α to Wi-Fi radiofrequency radiation for 5 hours influenced several bacterial cellular and metabolic processes.
    Mesh-Begriff(e) Electromagnetic Radiation ; Escherichia coli K12/genetics ; Escherichia coli K12/radiation effects ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial/radiation effects ; Software ; Transcriptome/radiation effects ; Wireless Technology
    Sprache Englisch
    Erscheinungsdatum 2019-10-08
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-019-51046-7
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Prevalence and antifungal susceptibility of Candida albicans causing vaginal discharge among pregnant women in Lebanon.

    Ghaddar, Nahed / Anastasiadis, Elie / Halimeh, Rawad / Ghaddar, Ali / Dhar, Rita / AlFouzan, Wadha / Yusef, Hoda / El Chaar, Mira

    BMC infectious diseases

    2020  Band 20, Heft 1, Seite(n) 32

    Abstract: Background: Vaginal candidiasis is frequent in pregnant women and is associated with sepsis and adverse neonatal outcomes. This study determined the prevalence of candida species in symptomatic pregnant women and evaluated the antifungal susceptibility ... ...

    Abstract Background: Vaginal candidiasis is frequent in pregnant women and is associated with sepsis and adverse neonatal outcomes. This study determined the prevalence of candida species in symptomatic pregnant women and evaluated the antifungal susceptibility profile of the isolated Candida strains. It also aimed to explore whether Candida species predicts gestational complications and adverse neonatal outcomes.
    Methods: A total of 258 pregnant women with vaginal discharge at 35 to 37 week of gestation participated in this study. Vaginal swabs from these patients were collected at various obstetrics and gynecology clinics in Lebanon for a period of 14 months. Candida isolates were identified at species level and antifungal susceptibility of Candida albicans to fluconazole (FCZ), amphotericin B (AMB), itraconazole (ICZ) and voriconazole (VCZ) was determined by the agar-based E-test method.
    Results: Among 258 women tested, 100 (39%) were positive for Candida species. C. albicans, C. glabrata and C. krusei were isolated from 42, 41 and 17% of the women, respectively. C. albicans was significantly associated only with gestational diabetes while C. krusei or C. glabrata had significant positive associations with other gestational complications. The antifungal susceptibility tests of C. albicans isolates revealed 97.5, 90, 87.5 and 97.5% susceptibility to AMB, FCZ, ICZ and VCZ, respectively.
    Conclusion: The current study revealed high incidence of both C. albicans and non-C. albicans Candida strains causing vulvovaginitis among pregnant women in Beirut, Lebanon. Candida screening as antenatal follow up is advised to minimize the risk of adverse neonatal outcome or gestational complications.
    Mesh-Begriff(e) Adult ; Amphotericin B/therapeutic use ; Antifungal Agents/therapeutic use ; Candida albicans/drug effects ; Candida albicans/isolation & purification ; Candida glabrata/drug effects ; Candida glabrata/isolation & purification ; Candidiasis, Vulvovaginal/drug therapy ; Candidiasis, Vulvovaginal/epidemiology ; Candidiasis, Vulvovaginal/microbiology ; Cross-Sectional Studies ; Drug Resistance, Fungal/drug effects ; Female ; Fluconazole/therapeutic use ; Follow-Up Studies ; Humans ; Incidence ; Infant, Newborn ; Itraconazole/therapeutic use ; Lebanon/epidemiology ; Microbial Sensitivity Tests ; Pregnancy ; Prevalence ; Vaginal Discharge/microbiology ; Voriconazole/therapeutic use
    Chemische Substanzen Antifungal Agents ; Itraconazole (304NUG5GF4) ; Amphotericin B (7XU7A7DROE) ; Fluconazole (8VZV102JFY) ; Voriconazole (JFU09I87TR)
    Sprache Englisch
    Erscheinungsdatum 2020-01-13
    Erscheinungsland England
    Dokumenttyp Journal Article
    ISSN 1471-2334
    ISSN (online) 1471-2334
    DOI 10.1186/s12879-019-4736-2
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Antimicrobial and biocide resistance of bacteria in a Lebanese tertiary care hospital.

    Jomha, Mariam Yousif / Yusef, Hoda / Holail, Hanafy

    Journal of global antimicrobial resistance

    2014  Band 2, Heft 4, Seite(n) 299–305

    Abstract: The environment in a Lebanese tertiary-level care hospital was examined for bacterial contamination. In total, 8 open-air and 62 surface samples were taken and CFU were enumerated. Morphologically different isolates (n=104) were obtained and tested for ... ...

    Abstract The environment in a Lebanese tertiary-level care hospital was examined for bacterial contamination. In total, 8 open-air and 62 surface samples were taken and CFU were enumerated. Morphologically different isolates (n=104) were obtained and tested for resistance to a wide range of antibiotics by the Kirby-Bauer disc diffusion method. Minimum bactericidal concentrations of isolates to seven different biocides used within this facility were also determined using both qualitative and quantitative methods. Gram-positive bacteria (82.7% of isolates) were encountered more often than Gram-negative bacteria (17.3%), and the genus Staphylococcus was most prevalent among isolates (59.6% of the isolates). Gram-positive isolates showed the highest resistance to penicillin (60.6%), clindamycin (54.5%) and ceftazidime (39.4%); the lowest frequencies of resistance were seen with vancomycin (0% of isolates), nitrofurantoin (3.5%), tetracycline (12.1%) and ciprofloxacin (12.1%). The prevalence of meticillin-resistant Staphylococcus aureus (MRSA) and meticillin-resistant coagulase negative staphylococci among all environmental samples was 3.8% and 11.5% of isolates, respectively. All Gram-negative isolates were multidrug-resistant (MDR). With respect to biocides, the highest frequency of resistance was to quaternary ammonium compounds (QACs) (13.5% of isolates) and the lowest frequency of resistance was to QAC/biguanide complex (<1% of isolates) and Anios DVA (3.8%). MDR bacteria can pose a serious threat in the hospital environment, showing the need for prudent use of antibiotics and correct infection control procedures.
    Sprache Englisch
    Erscheinungsdatum 2014-12
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 2710046-7
    ISSN 2213-7173 ; 2213-7165
    ISSN (online) 2213-7173
    ISSN 2213-7165
    DOI 10.1016/j.jgar.2014.09.001
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Phenotypic and Genotypic Characterization of Extended-Spectrum Beta-Lactamases Produced by

    Ghaddar, Nahed / Anastasiadis, Elie / Halimeh, Rawad / Ghaddar, Ali / Matar, Ghassan M / Abou Fayad, Antoine / Sherri, Nour / Dhar, Rita / AlFouzan, Wadha / Yusef, Hoda / El Chaar, Mira

    Infectious diseases in obstetrics and gynecology

    2020  Band 2020, Seite(n) 4190306

    Abstract: Introduction: Infections caused by extended spectrum beta lactamase (ESBL) producing bacteria continue to be a challenge for choosing the appropriate therapy since they may exhibit coresistance to many other classes of antibiotics. The aim of the study ... ...

    Abstract Introduction: Infections caused by extended spectrum beta lactamase (ESBL) producing bacteria continue to be a challenge for choosing the appropriate therapy since they may exhibit coresistance to many other classes of antibiotics. The aim of the study was to screen pregnant women for ESBL producing bacteria in Beirut, Lebanon, to examine their phenotypic and genotypic characterization and to study the association between ESBL colonization with adverse neonatal outcomes.
    Method: In this cross-sectional study, vaginal samples from 308 pregnant women at 35-37 weeks of gestation were studied during a one-year period. The samples were plated on MacConkey agar and selective MacConkey agar supplemented with ceftazidime. Phenotypic confirmation of ESBL production was performed by double-disc synergy test and all isolates were screened by PCR for the resistance genes bla
    Results: In total, 59 women out of 308 (19.1%) were colonized by ESBL producing gram negative bacteria. Two babies born to mothers colonized with ESBL were diagnosed with sepsis. The susceptibility rates of isolates to other antibiotics were 39% to co-trimoxazole, 49.2% to ciprofloxacin, 91.5% to gentamicin, 18.6% to aztreonam and 35.6% to cefepime. Most of isolates were highly sensitive to meropenem and imipenem, with a susceptibility of 93.2%. PCR was performed on all
    Conclusion: In conclusion, this study showed that Lebanon has a high prevalence of ESBL carriage in pregnant women. Further studies that include a continuous screening of pregnant women and follow up of their newborn clinical status should be conducted to foresee the risk of transmission.
    Mesh-Begriff(e) Anti-Bacterial Agents/pharmacology ; Carrier State/epidemiology ; Cross-Sectional Studies ; Escherichia coli/drug effects ; Escherichia coli/enzymology ; Escherichia coli/physiology ; Escherichia coli Infections/epidemiology ; Escherichia coli Infections/microbiology ; Female ; Genotype ; Humans ; Infant, Newborn ; Infectious Disease Transmission, Vertical ; Lebanon/epidemiology ; Microbial Sensitivity Tests ; Phenotype ; Pregnancy ; Prevalence ; Vagina/microbiology ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics
    Chemische Substanzen Anti-Bacterial Agents ; beta-Lactamases (EC 3.5.2.6)
    Sprache Englisch
    Erscheinungsdatum 2020-01-23
    Erscheinungsland Egypt
    Dokumenttyp Journal Article
    ZDB-ID 1176776-5
    ISSN 1098-0997 ; 1064-7449
    ISSN (online) 1098-0997
    ISSN 1064-7449
    DOI 10.1155/2020/4190306
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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