Artikel ; Online: Transient receptor potential vanilloid-1 (TRPV1) channels act as suppressors of the growth of glioma.
2024 Band 211, Seite(n) 110950
Abstract: The aim of this study was to investigate the expression and function of the transient receptor potential vanilloid 1 (TRPV1) in glioma. We found that the expression of TRPV1 mRNA and protein were upregulated in glioma compared with normal brain by qPCR ... ...
Abstract | The aim of this study was to investigate the expression and function of the transient receptor potential vanilloid 1 (TRPV1) in glioma. We found that the expression of TRPV1 mRNA and protein were upregulated in glioma compared with normal brain by qPCR and western blot analysis. In order to investigate the function of TRPV1 in glioma, short hairpin RNA (shRNA) and the inhibitor of TRPV1 were used. In vitro, the activation of TRPV1 induced cell apoptosis with decreased migration capability and inhibited proliferation, which was abolished upon TRPV1 pharmacological inhibition and silencing. Mechanistically, TRPV1 modulated glioma proliferation through the protein kinase B (Akt) signaling pathway. More importantly, in immunodeficient (NOD-SCID) mouse xenograft models, tumor size was significantly increased when TRPV1 expression was disrupted by a shRNA knockdown approach in vivo. Altogether, our findings indicate that TRPV1 negatively controls glioma cell proliferation in an Akt-dependent manner, which suggests that targeting TRPV1 may be a potential therapeutic strategy for glioma. |
---|---|
Mesh-Begriff(e) | Animals ; Humans ; Mice ; Apoptosis/physiology ; Brain Neoplasms/metabolism ; Brain Neoplasms/pathology ; Brain Neoplasms/genetics ; Cell Line, Tumor ; Cell Movement/physiology ; Cell Proliferation/physiology ; Glioma/genetics ; Glioma/metabolism ; Glioma/pathology ; Mice, Inbred NOD ; Mice, SCID ; Proto-Oncogene Proteins c-akt/metabolism ; RNA, Small Interfering/pharmacology ; Signal Transduction/physiology ; TRPV Cation Channels/metabolism ; TRPV Cation Channels/genetics |
Chemische Substanzen | Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; RNA, Small Interfering ; TRPV Cation Channels ; TRPV1 protein, human |
Sprache | Englisch |
Erscheinungsdatum | 2024-04-16 |
Erscheinungsland | United States |
Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 197620-5 |
ISSN | 1873-2747 ; 0361-9230 |
ISSN (online) | 1873-2747 |
ISSN | 0361-9230 |
DOI | 10.1016/j.brainresbull.2024.110950 |
Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
Zs.A 1243: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.