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  1. Artikel: Sample Preparation in Microbial Metabolomics: Advances and Challenges.

    Boness, Heiter V M / de Sá, Hanna C / Dos Santos, Emile K P / Canuto, Gisele A B

    Advances in experimental medicine and biology

    2023  Band 1439, Seite(n) 149–183

    Abstract: Microbial metabolomics has gained significant interest as it reflects the physiological state of microorganisms. Due to the great variability of biological organisms, in terms of physicochemical characteristics and variable range of concentration of ... ...

    Abstract Microbial metabolomics has gained significant interest as it reflects the physiological state of microorganisms. Due to the great variability of biological organisms, in terms of physicochemical characteristics and variable range of concentration of metabolites, the choice of sample preparation methods is a crucial step in the metabolomics workflow and will reflect on the quality and reliability of the results generated. The procedures applied to the preparation of microbial samples will vary according to the type of microorganism studied, the metabolomics approach (untargeted or targeted), and the analytical platform of choice. This chapter aims to provide an overview of the sample preparation workflow for microbial metabolomics, highlighting the pre-analytical factors associated with cultivation, harvesting, metabolic quenching, and extraction. Discussions focus on obtaining intracellular and extracellular metabolites. Finally, we introduced advanced sample preparation methods based on automated systems.
    Mesh-Begriff(e) Metabolome ; Reproducibility of Results ; Metabolomics/methods ; Specimen Handling
    Sprache Englisch
    Erscheinungsdatum 2023-10-16
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 410187-X
    ISSN 0065-2598
    ISSN 0065-2598
    DOI 10.1007/978-3-031-41741-2_7
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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    Verfügbar in ZB MED Köln/Königswinter

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  2. Artikel: Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens.

    de Souza, Hygor M R / Pereira, Tássia T P / de Sá, Hanna C / Alves, Marina A / Garrett, Rafael / Canuto, Gisele A B

    Metabolites

    2024  Band 14, Heft 1

    Abstract: This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal ... ...

    Abstract This review article compiles critical pre-analytical factors for sample collection and extraction of eight uncommon or underexplored biological specimens (human breast milk, ocular fluids, sebum, seminal plasma, sweat, hair, saliva, and cerebrospinal fluid) under the perspective of clinical metabolomics. These samples are interesting for metabolomics studies as they reflect the status of living organisms and can be applied for diagnostic purposes and biomarker discovery. Pre-collection and collection procedures are critical, requiring protocols to be standardized to avoid contamination and bias. Such procedures must consider cleaning the collection area, sample stimulation, diet, and food and drug intake, among other factors that impact the lack of homogeneity of the sample group. Precipitation of proteins and removal of salts and cell debris are the most used sample preparation procedures. This review intends to provide a global view of the practical aspects that most impact results, serving as a starting point for the designing of metabolomic experiments.
    Sprache Englisch
    Erscheinungsdatum 2024-01-05
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article ; Review
    ZDB-ID 2662251-8
    ISSN 2218-1989
    ISSN 2218-1989
    DOI 10.3390/metabo14010036
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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